اثر پیش تیمار پراکسیدهیدروژن و محلول‌پاشی مس بر برخی خصوصیات فیزیولوژیکی و عملکرد کمی ذرت هیبرید سینگل کراس 704 تحت تنش کمبود آب
محورهای موضوعی : ژنتیک
عبداله ایاران
1
,
محمدرضا دادنیا
2
,
مجتبی علوی فاضل
3
,
شهرام لک
4
,
طیب ساکی نژاد
5
1 - گروه زراعت، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران
2 - Department of Agronomy, Ahvaz Branch, Islamic Azad University, Iran
3 - گروه زراعت دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد اهواز
4 - هیات علمی دانشگاه آزاد اسلامی واحد اهواز و رئیس دانشگاه ازد اسلا می واحد اهواز
5 - گروه فیزیولوژی گیاهان زراعی، دانشگاه آزاد، اهواز، ایران
کلید واژه: ارتفاع بوته, عملکرد دانه, قطع آبیاری, کاتالاز, کاروتنوئید و کلروفیل a,
چکیده مقاله :
به‌منظور بررسی اثر کاربرد پیش تیمار پراکسیدهیدروژن و محلول‌پاشی مس بر برخی صفات فیزیولوژیکی و عملکرد کمی ذرت تحت تنش کمبود آب، آزمایشی به صورت کرت¬های دو بار خرد شده در قالب طرح بلوك‌هاي كامل تصادفي با چهار تکرار طی سال¬های زراعی 97-1396 و 98-1397 در مزرعه¬ای واقع در شهرستان باغملک اجرا شد. کرت اصلی رژیم آبیاری با 4 سطح نرمال، قطع آبیاری 40 ، 55 و 70 روز بعد از ظهور تاسل، کرت فرعی محلول‌پاشی مس با دو سطح شاهد و 30/0 کیلوگرم خالص در هکتار از منبع سولفات مس 50 درصد 25 روز بعد از ظهور تاسل و کرت فرعی فرعی تیمار بذر با پراکسید هیدروژن با 3 سطح شاهد،30 و 60 میلی‌مولار بود. نتایج نشان داد که برهم‌کنش سه‌گانه رژیم آبیاری، محلول‌پاشی مس و پراکسیدهیدروژن بر محتوی کلروفیل a، کلروفیل b، کارتنوئید کل، فعالیت کاتالازی، ارتفاع بوته، تعداد دانه در ردیف و عملکرد دانه در سطح احتمال یک درصد معنی¬دار بود. بیشترین ارتفاع بوته و تعداد دانه در ردیف از تیمارهای محلول‌پاشی مس و کاربرد 60 میلی‌مولار پراکسیدهیدروژن در شرایط آبیاری نرمال حاصل شد. همچنین بیشترین عملکرد دانه (07/9530 کیلوگرم در هکتار) در شرایط آبیاری نرمال و محلول‌پاشی مس و کاربرد 60 میلی‌مولار پراکسیدهیدروژن حاصل شد که نسبت به قطع آبیاری 40 روز بعد از ظهور تاسل و عدم محلول‌پاشی مس و عدم کاربرد پراکسیدهیدروژن حدود 42 درصد افزایش نشان داد. فعالیت کاتالازی در شرایط قطع آبیاری 40 روز بعد از ظهور تاسل و محلول‌پاشی مس و کاربرد 30 میلی‌مولار پراکسیدهیدروژن حدود 67 درصد نسبت به شرایط آبیاری نرمال و شاهد افزایش یافت. به¬طور کلی نتایج آزمایش نشان داد که محلول‌پاشی مس و کاربرد 60 میلی‌مولار پراکسیدهیدروژن در شرایط رطوبتی مناسب می¬تواند موجب افزایش مقدار کلروفیل، عملکرد دانه و در کل بهبود تولید ذرت شود.
In order to investigate the effect of hydrogen peroxide pretreatment and copper foliar application on some physiological traits and quantitative yield of corn under water deficit stress, a Split split plot experiment was conducted in a randomized complete block design with four replications, plants were planted in during two years (2017-19) in Baghmalek. The main plot of the irrigation regime with 4 normal levels, stopping irrigation 40, 55 and 70 days after the appearance of tassels, the sub-plot of copper foliar spraying with two control levels and 0.30 kg net per hectare from the source of copper sulfate 50% 25 days after the emergence of tassel and sub-plot of seed treatment with hydrogen peroxide with 3 control levels, 30 and 60 mM. The results showed that the triple interaction of irrigation regime, copper foliar application and hydrogen peroxide on chlorophyll a content, chlorophyll b, total carotenoid, catalase activity, plant height, number of seeds in a row and seed yield was significant at the probability level of 1%. The highest plant height and the number of seeds in the row were obtained from copper foliar spraying treatments and the application of 60 mM hydrogen peroxide under normal irrigation conditions. Also, the highest seed yield (9530.07 kg/ha) was obtained under normal irrigation conditions and copper foliar spraying and the application of 60 mM hydrogen peroxide, which showed an increase of about 42% compared to the cessation of irrigation 40 days after the emergence of tassels and the absence of copper foliar spraying and the absence of hydrogen peroxide application. The activity of catalase increased by 67% in the condition of stopping irrigation 40 days after the appearance of tassel and copper foliar application and application of 30 mM hydrogen peroxide compared to normal and control irrigation conditions. In general, the results of the experiment showed that copper foliar spraying and application of 60 mM hydrogen peroxide in suitable humidity conditions can increase chlorophyll content, grain yield and overall improvement of corn production.
Adebayo, M.A., and Menkir, A. (2014). Assessment of hybrids of drought tolerant maize (Zea mays L.) inbred lines for grain yield and other traits under stress managed conditions. Nigerian Journal of Genetics. 28: 19-23.
Apel, K., and Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress and signal transduction. Annual Review of Plant Biology. 55: 373-399.
Arnon, D. I. (1975). Physiological principles of dry land crop production. In: Gupta .U.S. (Ed). Physiological aspects of dry land farming. Pp. 3-14. Oxford press.
Ashraf, M. (2010). Inducing drought tolerance in plants: some recent advances. Biotechnology Advances. 28: 169-183.
Ben Ahmed, C.H., Ben Rouina, B., Sensoy, S., Boukhris, M., and Ben Abdallah, F. (2009). Changes in gas exchange, proline accumulation and antioxidative enzyme activities in three olive cultivars under contrasting water availability regimes. Environmental and Experimental Botany 67(2): 345-352.
Bernal, M., Cases, R., Picorel, R. and Yruela, I. (2007). Foliar and root Cu supply affect differently Fe and Zn uptake and photosynthetic activity in soybean plants. Environmental and Experimental Botany. 60: 145 -150.
Blasco, B., Leyva, R., Romero, L., and Ruiz, J. M. (2013). Iodine effects on phenolic metabolism in lettuce plants under salt stress. Journal of Agricultural and Food Chemistry. 61(11): 2591-2596.
Boominathan, R., and Doran, P.M. (2002). Ni indused oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii. Newphytologist. 156: 205-215.
Cakir, R. (2004). Effect of water stress at different development stages on vegetative and reproductive growth of corn. Field Crops Research. 89 (1): 1–16.
Chaves, M. M., Flexas, J., and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany. 103: 551–560.
Dimkpa, C.O., Bindraban, P.S., Fugice, J., Agyin-Birikorang, S., Singh, U., Hellums, D. (2017). Composite micronutrient nanoparticles and salts decrease drought stress in soybean. Agronomy for Sustainable Development. 37(1): 1-13.
Ellouzi, H., Sghayar, S., and Abdelly, C. (2017). H2O2 seed priming improves tolerance to salinity; drought and their combined effect more than mannitol in Cakile maritima when compared to Eutrema salsugineum. Journal of Plant Physiology. 210: 38-50.
Fatemi Ghiri, S.S., and Tabatabaei, S.J. (2022). Effect of hydrogen peroxide on growth and physiological characteristics of two tomato cultivars in hydroponic culture. Journal Soil and Plant Interactions. 12(4): 37-51. (In persian).
Feil, B., Moser, S.B., Jampatong, S., and Stamp, P. (2005). Mineral composition of the grains of tropical maize varieties as affected by preanthesis drought and rate of nitrogen fertilization. Crop Science. 45: 516–523.
Gaetke, L. M. and Chow, C. K. (2003). Copper toxicity, oxidative stress, and antioxidant nutrients. Toxicology. 189: 147 -197.
Ghassemi-Golezani, K., Heydari, Sh., Dalil, B. (2018). Field performance of maize (Zea mays L.) cultivars under drought stress. Acta agriculturae Slovenica. 111(1): 25-32.
Ghete, A.B., Duda, M.M., Vârban, D.I., Varban, R., Moldovan, C., and Muntean, S. (2018). Maize (Zea mays), a prospective medicinal plant in Romania. Hop and Medicinal Plants. 26: 44-51.
Giancarla, V., Madosa, E., Ciulca, S., Coradini, R., Iuliana, C., Mihaela, M. and Lazar, A. (2013). Influence of water stress on the chlorophyll content in barley. Journal of Horticulture, Forestry and Biotechnology. 17: 223-228.
Gill, S.S. and Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry. 48: 909-930.
Gondim, F. A., Miranda, R. D., Gomes-Filho, E., and Prisco, J. T. (2013). Enhanced salt tolerance in maize plants induced by H2O2 leaf spraying is associated with improved gas exchange rather than with non-enzymatic antioxidant system. Theoretical and Experimental Plant Physiology. 25: 251-260.
Hashemzadeh, F. (2009). Effects of drought stress and cycocel application on yield of maize varieties in second crop. Agroecology Journal. 5(14): 67-79. (In persian with english abstract).
Hosseinpour, A., Mahalleh, Kh., Roshdi, M., and Alinezhad, N. (2015). Evaluation of micronutrient application method and irrigation stopping on yield and yield component of grain Corn in Urmia (North-West Of Iran). Advances in Environmental Biology. 9(24): 431-437.
Houshmandfar, A., and Moraghebi, F. (2011). Effect of mixed cadmium, copper, nickel and zinc on seed germination and seedling growth of safflower. Africa Journal Agriculture Research. 6(5): 1182-1187.
Ijaz, R., Ejaz, J., Gao, S., Liu, T., Imtiaz, M., Ye, Z., and Wang, T. (2017). Overexpression of annexin gene AnnSp2, enhances drought and salt tolerance through modulation of ABA synthesis and scavenging ROS in tomato. Scientific Reports. 7: 12087. doi:10.1038/ s41598-017-11168-2.
Jafarian, T., Zarea, J., Siosemardeh, A. (2019). Flag leaf anatomical characteristics and the spike pretreatment changes with hydrogen peroxide and its relationship with grain yield in dry land conditions. Journal of Plant Process and Function. 7(27): 290-303. (In persian).
Kabata-Pendias, A., and Pendias, H. (1992). Trace Elements in Soils and Plants. 2nd Edition, CRC Press, Boca Raton.
Karimi, D.K., Mojaddam, M., Lack, Sh., Payandeh, Kh., and Shokuhfar, A.R. (2021). The effect of superabsorbent and iron and zinc foliar application on antioxidant enzyme activity and yield maize (S.C.704) (Zea mays L.) under irrigation regimes. Environmental Stresses in Crop Sciences. 14(2): 387-402. (In persian).
Lee, W.M., An, Y.J., Yoon, H., Kweon, H.S. (2008). Toxicity and bioavailability of copper nanoparticles to the terrestrial plants bean (phaseolus radiatus) and wheat, Plant agar test for water-insoluble nanoparticles. Environmental toxicology and chemistry. 27(9):1915-1921.
Liu, J., Guo, Y.Y., Bai, Y.W., Xue, J.Q., and Zhang , R.H. (2018). Effects of Drought Stress on the Photosynthesis in Maize. Russian Journal Plant Physiology. 65: 849–856.
Mudgal, V., Madaan, N., and Mudgal, A. (2010). Biochemical mechanisms of salt tolerance in plants: A Review. International Journal of Botany. 6(2): 136-143.
Naderi, T., and Sohrabi, U. 2019. Effect of bio and chemical fertilizers on some physiological traits of maize (Zea mays L.) under drought stress. Environmental Stresses in Crop Science. 12(4): 1205-1224. (In persian).
Nobakht, P., Ebadi, A., Parmoon, Gh., and Nickhah Bahrami, R. (2018). Study role H2O2 in Photosynthetic pigments Peppermint (Mentha piperita L.) on Water stress conditions. Journal of Plant Process and Function. 7(27): 19-32. (In persian).
Nohtani, H., and Mahdinezhad, N. (2020). Evaluation of salinity stress tolerance based on biochemical and morphophysiological characteristics of some wheat cultivars. Iranian Journal of Seed Sciences and Research.7(1): 55-67. (In persian).
Rad, M.R.N., Kadir, M.A., Yusop, M.R. (2012). Genetic behaviour for plant capacity to produce chlorophyll in wheat (Triticum aestivum) under drought stress. Australian Journal of Crop Science. 6(3): 415-420.
Sadeghipour, O., and Aghaei, P. (2012). Response of common bean to exogenous application of salicylic asid under water stress conditions. Advances in Environmental Biology. 6(3): 1160-1168.
Sah, R. P., Chakraborty, M., Prasad, K., Pandit, M., Tudu, V. K., Chakravarty, M. K., Narayan, S. C., Rana, M., and Moharana, D. (2020). Impact of water deficit stress in maize: Phenology and yield components. Scientific Reports. 10: 1-15.
Santhy, V., Meshram, M., Wakde, R., and Vijaya Kumari, R. (2014). Hydrogen peroxide pretreatment for seed enhancement in cotton. African Journal of Agricultural Research. 9: 1982-1989.
Sharma. P., Ambuj, B.J., Rama, S.D. and Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal Botany. 26 Pp.
Tabatabaei, S.A., and Shakeri, E. (2016). Effect of drought stress on maize hybrids yield and determination of the best hybrid using drought tolerance indices. Environmental stresses in crop sciences. 8(1): 121-125. (In persian with english abstract).
Yusefzaei, F., Poorakbar, L., farhadi, Kh., and Molaei, R. (2017). The Effect of copper nanoparticles and copper chloride solution on germination and solution some morphological and physiological factors (Ocimum basilicum L.). Journal of Plant Research. 30(1): 221-232. (In persian).