تغییر محتوای فیتوشیمیایی و فعالیت آنتی اکسیدانتی گیاه دارویی کلماتیس اصفهانی (Clematis ispahanica Boiss.) در سه رویشگاه طبیعی استان سمنان
محورهای موضوعی : گیاهان دارویی
محمد مهدی ذوالفقاری
1
(گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران، )
رضا نادری
2
(گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران، )
عاطفه امیر احمدی
3
(گروه علوم گیاهی، دانشکده زیست شناسی، دانشگاه دامغان، دامغان، ایران، )
کلید واژه: اکوفیزیولوژی, تنش شوری, سنجش‌های بیوشیمیایی, زیستگاه, گیاهان دارویی,
چکیده مقاله :
در این مطالعه پتانسیل آنتی‌اکسیدانتی و خواص فیزیولوژیکی گیاه کلماتیس اصفهانی از رویشگاه‌های مختلف استان سمنان بررسی شد. سه رویشگاه طبیعی این گیاه در استان سمنان (چشمه علی، دیباج و مجن) شناسایی و نمونه برداری از برگ گیاهان صورت گرفت. میزان کلروفیل، کاروتنوئید، آنتوسیانین، پرولین، پروتئین، فنول، فلاونوئید، قندهای احیاکننده، پراکسیدهیدروژن، مالون دی آلدهید و فعالیت آنزیم‌های کاتالاز و گایاکول پراکسیداز اندازه گیری شد. همچنین خواص آنتی اکسیدانتی گیاهان با اسـتفاده از ظرفیـت مهـار رادیکال‌هايDPPH مورد بررسی قرار گرفت. آنالیز خاک هر سه رویشگاه نیز انجام شد. آزمایش به‌صورت طرح کاملاً تصادفی با 3 تکرار و مقایسـه میـانگین داده‌هـا بـا استفاده از آزمون دانکن در سطح احتمال 5 درصد انجام شد. نتایج نشان داد که مقدار متابولیت‌های گیاه شامل قندهای محلول، ترکیبات فنولی و فلاونوئیدها، فعالیت آنزیم کاتالاز و خواص آنتی اکسیدانتی در رویشگاه چشمه علی در بالاترین و در رویشگاه مجن در کمترین میزان قرار دارد. در مقابل مقدار ترکیبات دفاعی گیاه شامل پرولین، پراکسیدهیدروژن، مالون دی آلدهید و فعالیت آنزیم گایاکول پراکسیداز در رویشگاه چشمه علی در کمترین و در رویشگاه مجن در بالاترین میزان قرار دارد. مقدار کلروفیل، کارتنوئیدها، آنتوسیانین و پروتئین نیز در رویشگاه دیباج با بالاترین ارتفاع از سطح دریا در کمترین میزان قرار دارد. نتایج این تحقیق نشان داد که رویشگاه چشمه علی با داشتن بیشترین میزان متابولیت‌ها و کمترین میزان ترکیبات دفاعی کم تنش‌ترین رویشگاه و بالعکس رویشگاه مجن پرتنش‌ترین رویشگاه برای گیاه کلماتیس اصفهانی می‌باشد.
In this study the antioxidant activity and physiological properties of Clematis ispahanica from different habitats of Semnan province were investigated. Three natural habitats of this plant were identified in Semnan province (Cheshmeh Ali, Dibaj and Mojen) and leaf were sampled from plants. Chlorophyll, carotenoid, anthocyanin, proline, protein, phenol, flavonoid, reducing sugars, hydrogen peroxide, malondialdehyde, catalase and guaiacol peroxidase activities were measured. Also, the antioxidant properties of plants were investigated using DPPH radical scavenging capacity. Soil analysis of all three habitats was also done. The experiment was carried out as a completely random design with 3 repetitions and comparison of the average data using Duncan's test at a probability level of 5%. The results showed that the amount of plant metabolites, including soluble sugars, phenolic compounds and flavonoids, catalase enzyme activity and antioxidant properties was the highest in Cheshmeh Ali habitat and the lowest in Mojen habitat. On the other hand, the amount of plant defense compounds including proline, hydrogen peroxide, malondialdehyde and guaiacol peroxidase enzyme activity was the lowest in Cheshmeh Ali habitat and the highest in Mojen habitat. The amount of chlorophyll, carotenoids, anthocyanin and protein was also the lowest in the Dibaj habitat with the highest altitude above sea level. The results of this research showed that the Cheshmeh Ali habitat with the highest amount of metabolites and the lowest amount of defense compounds is the least stressful habitat, and conversely, the Mojen habitat is the most stressful habitat for C. ispahanica.
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