بهینه¬سازی محیط کشت شیمیائی صنعتی جهت کشت کنسرسیوم جلبکی با کاربری کود بیولوژیکی
محورهای موضوعی : ریزجلبکساسان قبادیان 1 , ندا سلطانی 2 , پیروز شکری خطیبی 3
1 - گروه مهندسی عمران، دانشکده مهندسی عمران و معماری، دانشگاه ملایر، ملایر، ایران
2 - گروه تحقیق و توسعه، شرکت آفاق نگران زیست¬گستر، تهران، ایران
3 - گروه مهندسی مکانیک بیوسیستم، دانشکده کشاورزی، دانشگاه تربیت¬مدرس، تهران، ایران
کلید واژه: بهینه سازی کشت, کشت ریزجبلک, کلروفیل, کود زیستی, محیط کشت BG11, نرخ رشد مخصوص,
چکیده مقاله :
با نگاه به مفاهیم اصلی توسعه پایدار، نیاز روزافزون به منابع غذائی در کنار مضراتِ محیط¬زیستیِ بکارگیری کودهای شیمیائی، لزوم تولید کود¬های زیستی آنهم با بهره¬گیری از ریزجلبک¬ها به عنوان کارخانه¬های سلولی بیولوژیکی کارآمد را روشن می¬سازد. در این پژوهش با هدف ارتقاء بهره¬وری جهت کشت تجاری یک کنسرسیوم جلبکی (که نقش موثر آن در رشد و نمو محصولات کشاورزی در پژوهش موازی اثبات شده) بهینه¬سازی محیط¬کشت BG11 با تکیه بر چهار ترکیب K2HPO4، NaCl ، CaCl2 و FeNH4Cit مد نظر قرار گرفت. از روش سطح پاسخ برای طراحی فاکتورها و تحلیل پاسخ¬ها استفاده شد و سنجش¬های وزن خشک در روز¬های 4، 8، 11 و 15 کشت همراه با محاسبه نرخ رشد مخصوص و میزان کلروفیل انجام شد. بیشترین میزان وزن خشک mg/L 1.15 در آزمایشی با کمترین میزان شوری به دست آمد در حالیکه سایر فاکتورها در مقادیر میانی بازه تغییرات خود قرار داشتند. اندرکنش شدید مواد مغذی و حساسیتِ نرخ رشد مخصوص نسبت به این اندرکنش مشهود بود. بیشترین میزان کلروفیل، در مقادیر میانی K2HPO4 در کنار کمترین مقدار سایر فاکتورها به دست آمد. بر اساس نتایج، بدلیل اثرگذاری شدید مواد بر روی یکدیگر و تعامل پیچیده بین عوامل محیطی و فیزیولوژی ارگانیسم‌های فتوسنتزی، به نظر می¬رسد حفظ تعادل بین ترکیبات موثرتر از مصرف مقادیر بالاتر آنها باشد و با بهره¬گیری از این تعادل میتوان با کاهش مصرف و افزایش بهره¬وری، صرفه¬جویی اقتصادی مورد نظر در هزینه کشت را انجام داد.
With a look at the fundamental concepts of sustainable development, the increasing need for food resources alongside the environmental hazards of using chemical fertilizers highlights the necessity of producing organic fertilizers, especially utilizing microalgae as efficient biological cellular factories. In this study, seeking to enhance productivity for the commercial cultivation of an algae consortium (parallel research have proved its significant role in the growth and development of agricultural products), optimization of the BG11 culture medium was considered based on four combinations of K2HPO4, NaCl, CaCl2 and FeNH4Cit. The response surface methodology was employed to design the factors and analyze the responses. Dry weights on days 4, 8, 11 and 15 of cultivation were measured; Specific growth rates and chlorophyll levels calculation were accompanied. The maximum dry weight of 1.15 mg/L was obtained in the experimental group with the lowest salinity, while other factors fluctuated within intermediate ranges. The strong interaction of nutrients and the sensitivity of specific growth rates to this interaction were evident. The highest chlorophyll levels were achieved with intermediate levels of K2HPO4 alongside the lowest levels of other factors. Based on the results, maintaining balance among the components appears to be more effective than consuming higher amounts of them. By leveraging this balance, it is possible to achieve the desired economic savings in cultivation costs through reduced consumption and increased productivity.
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