Antibacterial effects of green laser in vicinity of silver nanoparticles on methicillin-resistant staphylococcus aureus (MRSA)

Subject Areas : Nano Biophotonics

Maliheh  Ranjbaran ¹ (Department of physics, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
Zahra  Aghaebrahimi ² (Department of Physics, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
Jamshid  Sabaghzadeh ³ (Department of Physics, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
zohreh  daraeizadeh ⁴ (Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)
arash  abednezhad ⁵ (Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran)

Received: 2021-10-30 Accepted : 2022-03-07 Published : 2021-07-01

Keywords: silver nanoparticles, Antibacterial activity, Green laser, Methicillin-resistant S. aureus (MRSA), Oxygen species,

Abstract :

Methicillin-resistant Staphylococcus aureus (MRSA) is a type of staph bacteria that is resistant to most of the available antibiotics. So different methods have been introduced to overcome this great threat to public health. Photoactivated metal nanoparticles have been presented as good alternatives to antibiotics. In this paper, S. aureus ATCC 33591 as reference strains and clinical MRSA bacteria, isolated from wounds of burn patients, was used. After identification of the bacteria and testing its antibiotic susceptibility, the minimum inhibitory concentration test was performed to study the antibacterial effect of Ag-NPs on MRSA. Our results showed that the bacterial colony population of both samples of S. aureus strains was reduced significantly in the presence of Ag-NPs. The antibacterial effect was enhanced by utilizing a green laser. Interestingly, the bacterial growth has completely inhibited after 2 minutes of laser irradiation in the BHI agar medium. Also, in the nutrient agar medium and Müller-Hinton agar medium, significant synergistic bacterial suppression has been observed by combination the antibacterial effects of the laser and the Ag-Nps.

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