In vitro Estimation of Microbial Nitrogen Production and Ruminal Fermentation Responses to Levels and Applying Duration of a Commercial Enzyme Mixture

Subject Areas : Camel

ع.  فرامرزی-گرمرودی ¹ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
م.  دانش مسگران ² (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ع.ر.  وکیلی ³ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)

Received: 2015-04-27 Accepted : 2015-05-31 Published : 2016-03-01

Keywords: methane, Enzyme, degradability, microbial nitrogen,

Abstract :

The objective of the present experiment was to quantify the gas production parameters, methane emission and microbial nitrogen production of a conventional total mixed ration including corn silage, alfalfa hay, wheat straw and concentrate (390, 110, 22 and 478 g/kg of diet DM, respectively) treated with different levels of Natuzyme® and pre-incubation time after enzyme administration. Two hundred fifty mg of milled and dried basal diet (n=4) was weighed into a 125 mL serum bottles for an in vitro gas production trial. A solution of a commercial enzyme blend (Natuzyme®) was added at 0, 12 and 24 h prior to starting the incubation (96 h) to make treatments of 0.84, 1.68 and 2.52 g/kg, respectively. No added enzyme bottles were considered as control. Half time of gas production (t1/2) was calculated. Then, another gas test was run for each treatment and terminated at t1/2 to measure gas volume, methane emission and solid residues. Dose rate of Natuzyme® did not make any significant differences in gas production parameters and fermentation responses except for microbial nitrogen production. Pre-treatment of the basal diet at 24 h incubation significantly (P<0.05) lowered dry matter degradability and accumulated gas, but percentage and amount of produced methane, produced methane by mg degraded dry matter and microbial nitrogen production was significantly increased. Twelve and twenty four h pre-treatment of the basal diet significantly (P<0.05) increased accumulated gas by 16% and 8%, respectively. However, fractional constant rate was significantly (P<0.05) decreased by 31% and 13%, respectively, using twelve and twenty four h pre-treatment of the basal diet. However, a specific pre-treatment time must be taken into consideration to allow the enzyme mixture to bind to the feed particles.

References:

Almaraz I., Gonzalez S.S., Pinos-Rodriguez J.M. and Miranda L.A. (2010). Effects of exogenous fibrolytic enzymes on in sacco and in vitro degradation of diets and on growth performance of lambs. Italian J. Anim. Sci. 9, 6-10.

AOAC. (1990). Official Methods of Analysis. Vol. I. 15^th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.

Baloyi T.F. (2008). Effects of exogenous fibrolytic enzymes on in vitro fermentation kinetics of forage and mixed feed substrates. MS Thesis. StellenboschUniv., Stellenbosh, South Africa.

Beauchemin K.A., Rode L.M. and Sewalt V.J.H. (1995). Fibrolytic enzymes increase fibre digestibility and growth rate of steers fed dry forages. Canadian J. Anim. Sci. 75, 641-644.

Beauchemin K.A., Rode L.M., Maekawa M., Morgavi D.P. and Kampen R. (2000). Evaluation of a non-starch polysaccharidase feed enzyme in dairy cow diets. J. Dairy Sci. 83, 543-553.

Beauchemin K.A., Yang W.Z. and Rode L.M. (1999). Effects of grain source and enzyme additive on site and extent of nutrient digestion in dairy cows. J. Dairy Sci. 82, 378-390.

Beauchemin K.A., Colombatto D., Morgavi D.P., Yang W.Z. and Rode L.M. (2004). Mode of action of exogenous cell wall degrading enzymes for ruminants. Canadian J. Anim. Sci. 84, 13-22.

Beauchemin K.A., Colombatto D., Morgavi D.P. and Yang W.Z. (2003). Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. J. Amin. Sci. 81(2), 37-47.

Beever D.E. (1993). Ruminant animal production from forages: present position and future opportunities. Pp. 158-164 in Grasslands for Our World. SIR Publishing, Wellington, New Zealand.

Blummel M. and Lebzien P. (2001). Predicting ruminal microbial efficiencies of dairy ration by in vitro techniques. Livest. Prod. Sci. 68, 107-117.

Colombatto D., Mould F.L., Bhat M.K. and Owen E. (2003). Use of fibrolytic enzymes to improve the nutritive value of ruminant diets: a biochemical and in vitro rumen degradation assessment. Anim. Feed Sci. Technol. 107, 201-209.

Cruywagen C.W. and Goosen L. (2004). Effect of an exogenous fibrolytic enzyme on growth rate, feed intake and feed conversion ratio in growing lambs. South African J. Anim. Sci. 34(2), 71-73.

Dhiman T.R., Zaman M.S., Gimenez R.R., Walters J.L. and Treacher R. (2002). Performance of dairy cows fed forage treated with fibrolytic enzymes prior to feeding. Anim. Feed Sci. Technol. 101, 115-125.

Dong Y., Bae H.D., McAllister T.A., Mathison G.W. and Cheng K.J. (1999). The effect of exogenous fibrolytic enzymes, α-bromoethanesulfonate and monensin on digestibility of grass hay ans methane production in the Rusitec. Canadian J. Anim. Sci. 79, 491-498.

Eun J.S., Fellner V. and Gumpertz M.L. (2004). Methane production by mixed ruminal cultures incubated in dual-flow fermenters. J. Dairy Sci. 87, 112-121.

Eun J.S. and Beauchemin K.A. (2007). Assessment of the efficacy of varying experimental exogenous fibrolytic enzymes using In vitro fermentation characteristics. Anim. Feed Sci. Technol. 132, 298-315.

Eun J.S., Beauchemin K.A. and Schulze H. (2007). Use of exogenous fibrolytic enzymes to enhance in vitro fermentation of alfalfa hay and corn silage. J. Dairy Sci. 90, 1440-1451.

Faramarzi-Garmroodi A., Danesh Megaran M., Parand E. and Vakili A.R. (2013). In vitro effect of increasing levels of natuzyme® on fermentation responses of corn silage based diet. Annu. Rev. Res. Biol. 3(4), 1066-1073.

Feng P., Hunt C.W., Pritchard G.T. and Julien W.E. (1996). Effect of enzyme preparations on in situ and in vitro degradation and in vivo digestive characteristics of mature cool-season forage in beef steers. J. Anim. Sci. 74, 1349-1357.

Feng P., Hunt C.W., Julien W.E., Dickinson K. and Moen T. (1992). Effect of enzyme additives on in situ and in vitro degradation of mature cool-season grass forage. J. Anim. Sci. 70(1), 309-407.

Giraldo L.A., Tejido M.L., Ranilla M.J. and Carro M.D. (2008). Effects of exogenous fibrolytic enzymes on in vitro ruminal fermentation of substrates with different forage: concentrate ratios. Anim. Feed Sci. Technol. 141, 306-325.

Giraldo L.A., Tejido M.L., Ranilla M.J. and Carro M.D. (2007). Influence of exogenous fibrolytic enzymes and fumarate on methane production, microbial growth and fermentation in Rusitec fermenters. Br. J. Nutr. 98, 753-761.

Goering H.K. and Van Soest P.J. (1970). Forages fibre analysis: aparatus, reagents, Procedures and some applications. Agricultural Handbook No. 379, ARS-USDA, Washington, US.

Grings E.E., Blummel M. and Sudekum K.H. (2005). Methodological considerations in using gas production techniques for estimating ruminal microbial efficiencies for silage-based diets. Anim. Feed Sci. Technol. 123, 527-545.

Hristov A., McAllister T.A. and Cheng K.J. (1998). Exogenous enzymes for ruminants: modes of action and potential applications. Pp. 33-39 in Proc. 17^thWestern Nutr. Conf. Edmonton, Alberta, Canada.

Hristov A.N., Rode L.M., Beauchemin K.A. and Wuerfel R.L. (1996). Effect of a commercial enzyme preparation on barley silage in vitro and in sacco dry matter degradability. Pp. 282-284 in Proc.West. Sect. Meet. Am. Soc. Anim. Sci. Las Cruces, New Mexico.

Jalilvand G., Odongo N.E., López S., Naserian A., Valizadeh R., Eftekhar Shahrodi F., Kebreab E. and France J. (2008). Effects of different levels of an enzyme mixture on in vitro gas production parameters of contrasting forages. Anim. Feed Sci. Technol. 146, 289-301.

Krause M., Beauchemin K.A., Rode L.M., Farr B.I. and Norgaard P. (1998). Fibrolytic enzyme treatment of barley grain and source of forage in high-grain diets fed to growing cattle. J. Anim. Sci. 76, 2912-2920.

Krueger N.A., Adesogan A.T., Staples C.R., Krueger W.K., Dean D.B. and Littell R.C. (2008). The potential to increase digestibility of tropical grasses with a fungal, ferulic acid esterase enzyme preparation. Anim. Feed Sci. Technol. 145, 95-108.

Kung L., Littell R.J., Nauman G.A., Smagala A.M., Endres K.M. and Endres M.A. (2000). The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance of dairy cows. J. Dairy Sci. 83, 115-122.

Kung L., Cohen M.A., Rode L.M. and Treacher R.J. (2002). The effect of fibrolytic enzymes sprayed onto forages and fed in a total mixed ratio to lactating dairy cows. J. Dairy Sci. 85, 2396-2402.

Lewis G.E., Hunt C.W., Sanchez W.K., Treacher R., Pritchard G.T. and Feng P. (1996). Effect of direct-fed fibrolytic enzymes on the digestive characteristics of a forage-based diet fed to beef steers. J. Anim. Sci. 74, 3020-3028.

McAllister T.A., Hristov A.N., Beauchemin K.A., Rode L.M. and Cheng K.J. (2001). Enzymes in ruminant diets. Pp. 273-298 in Enzymes in Farm Animal Nutrition. M.R. Bedford and G.G. Partridge, Eds. CAB International, UK.

McGinn S.M., Beauchemin K.A., Coates T. and Colombatto D. (2004). Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast and fumaric acid. J. Anim. Sci. 82, 3346-3356.

Moharrery A., Hvelplund T. and Weisbjerg M.R. (2009). Effect of forage type, harvesting time and exogenous enzyme application on degradation characteristics measured using in vitro technique. Anim. Feed Sci. Technol. 153, 178-192.

Morgavi D.P., Beauchemin K.A., Nsereko V.L., Rode L.M., Iwaasa A.D., Yang W.Z., McAllister T.A. and Wang Y. (2000). Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum. J. Dairy Sci. 83, 1310-1321.

Newbold J. (1997). Proposed mechanisms for enzymes as modifiers of ruminal fermentation. Pp. 146-159 in Proc. 8^th Annu. Florida Rumin. Nutr. Symp. Gainesville, Florida, USA.

Nsereko V.L., Morgavi D.P., Rode L.M., Beauchemin K.A. and McAllister T.A. (2000). Effects of fungal enzyme preparations on hydrolysis and subsequent degradation of alfalfa hay fibre by mixed rumen microorganisms in vitro. Anim. Feed Sci. Technol. 88, 153-170.

Nsereko V.L., Beauchemin K.A., Morgavi D.P., Rode L.M., Furtado A.F., McAllister T.A., Iwaasa E.A., Yang W.Z. and Yang Y. (2002). Effect of a fibrolytic enzyme from Trichoderma longibrachiatum on the rumen population of dairy cows. Canadian J. Microbiol. 48, 14-20.

Orskov E.R. and McDonaldI. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. J. Agric. Sci. 92, 499-503.

Parand E., Danesh Mesgaran M., Faramarzi-Garmroodi A. and Vakili A.R. (2014). Effect of level and duration of application of a commercial enzyme mixture on in vitro ruminal fermentation responses of a mid-forage total mixed ration. Anim. Nutr. Feed Technol. 14, 109-119.

SAS Institute. (1999). SAS^®/STAT Software, Release 8. SAS Institute, Inc., Cary, NC. USA.

Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.

Yang W.Z., Beauchemin K.A. and Rode L.M. (2000). A comparison of methods of adding fibrolyticenzymes to lactating cow diets. J. Dairy Sci. 83, 2512-2520.

Yang W.Z., Beauchemin K.A. and Rode L.M. (1999). Effects of an enzyme feed additive on the extent of digestion and milk production of lactating dairy cows. J. Dairy Sci. 82, 391-403.