• Home
  • The effect of extraction method (ultrasonic, maceration and soxhlet) and solvent type on the extraction rate of phenolic compounds and extraction efficiency of Arctium lappa L. roots and Polygonum aviculare L. grass

Share To

Article Url


Manuscript ID : FH-2106-1076 Visit : 179 Page: 28 - 34

Article Type: Original Research

The effect of extraction method (ultrasonic, maceration and soxhlet) and solvent type on the extraction rate of phenolic compounds and extraction efficiency of Arctium lappa L. roots and Polygonum aviculare L. grass

Subject Areas :

Zahra Farahani 1

1 - Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2020-12-16 Accepted : 2021-05-23 Published : 2021-04-01

Keywords: Soxhlet, Ultrasonic bath, Maceration, <i>Arctium lappa</i> L. roots, <i>Polygonum aviculare</i> L. grass,

Abstract :

The species of Arctium lappa L. roots and Polygonum aviculare L. grass have various therapeutic properties in traditional medicine in East Asian countries. The aim of this study was to investigate the effect of three methods of extracting extracts by ultrasonic bath, maceration, and soxhlet to determine phenolic compounds of Arctium lappa L. roots and Polygonum aviculare L. grass using water and 50% ethanol and the evaluation of extraction efficiency of their extract. The results showed that in the ultrasonic bath extraction method, ethanolic extract of Arctium lappa L. root had the highest content of phenolic compounds (976 mg equivalent to Gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to Polygonum aviculare L. grass when using both solvents (14%). In the maceration extraction method, the ethanolic extract of Arctium lappa L. root showed the highest content of phenolic compounds (976 mg equivalent to Gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to the Polygonum aviculare L. grass extracted with water (12%). Also, in the Soxhlet extraction method, ethanolic extract of Arctium lappa L. root showed the highest content of phenolic compounds (578 mg equivalent of gallic acid per gram of dry sample), and the highest extraction efficiency in this method was related to the Polygonum aviculare L. grass extracted with ethanol (18/16%). The content of extraction of bioactive compounds based on the type of solvent, the method, and the type of plant used were significantly different from each other and 50% ethanol solvent was the best solvent for extracting the desired compounds. Arctium lappa L. roots also had the highest phenolic composition and extraction by maceration, soxhlet, and ultrasonic bath methods had the most significant effect on the extraction of phenolic compounds, respectively.

References:


  1. Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, Bakrouf A, Magné C, Abdelly C.  Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical Toxicology. 2009;47(8):2083-91.
    2.
  2. Owen RW, Giacosa A, Hull WE, Haubner R, Spiegelhalder B, Bartsch H. The antioxidant/anticancer potential of phenolic compounds isolated from olive oil. European Journal of Cancer. 2000;36(10):1235-47.
    3.
  3. Kris-Etherton PM, Lichtenstein AH, Howard BV, Steinberg D, Witztum JL. Antioxidant vitamin supplements and cardiovascular disease. Circulation. 2004;110(5):637-41.
    4.
  4. Mahdavi DL, Deshpande SS, Salunkhe DK. Food Antioxidant. Marcel Dekker Inc., New York. USA. 1995; 746.
    5.
  5. Khoshdouni Farahani, Z., Ebrahimzadeh Mousavi, M., Khoshdouni Farahani, F. The effect of extraction conditions using ultrasonic and maceration methods on the extraction rate of phenolic compounds and the extraction efficiency of jujube fruit (Ziziphus spp.). Applied Biology. 2021;11(41).
    6.
  6. Habibi RM, Mohammadi RA, Delazar A, Halabian R, Soleimani RJ, Mehdipour A, Bagheri M, Jahanian-Najafabadi A. Effects of Polygonum aviculare herbal extract on proliferation and apoptotic gene expression of MCF-7. Daru: Journal of Faculty of Pharmacy, Tehran University of Medical Sciences. 2011;19(5):326.
    7.
  7. Zhang H, Yu M, Jia H, Zhang T, Shang H, Zhang M, Zhu Z, Zou Z. Comprehensive identification of potential antioxidant components in the aerial parts of Polygonum chinese L. var. hispidum using ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry. Journal of Separation Science. 2020;43(12):2380-92.
    8.
  8. Hsu CY. Antioxidant activity of extract from Polygonum aviculare L. Biological Research. 2006;39(2):281-8.
    9.
  9. Wang KJ, Zhang YJ, Yang CR. Antioxidant phenolic compounds from rhizomes of Polygonum paleaceum. Journal of Ethnopharmacology. 2005;96(3):483-7.
    10.
  10. Datta BK, Datta SK, Rashid MA, Nash RJ, Sarker SD. A sesquiterpene acid and flavonoids from Polygonum viscosum. Phytochemistry. 2000;54(2):201-5.
    11.
  11. Fan D, Zhou X, Zhao C, Chen H, Zhao Y, Gong X. Anti-inflammatory, antiviral and quantitative study of quercetin-3-O-β-D-glucuronide in Polygonum perfoliatum L. Fitoterapia. 2011;82(6):805-10.
    12.
  12. Sakat S, Tupe P, Hule A, Juvekar A. Anti-inflammatory potential of flavonoid fraction of Tamarindus indica Linn (seeds). Planta Medica. 2010;76(12):SL-20.
    13.
  13. Granica S, Czerwińska ME, Żyżyńska-Granica B, Kiss AK. Antioxidant and anti-inflammatory flavonol glucuronides from Polygonum aviculare L. Fitoterapia. 2013;91:180-8.
    14.
  14. Petkova N, Ivanov I, Mihaylova D, Lante A. Effect of pressure liquid extraction and ultrasonic irradiation frequency on inulin, phenolic content and antioxidant activity in burdock (Arctium lappa L.) roots. Acta Scientiarum Polonorum. Hortorum Cultus. 2020;19(3).
    15.
  15. Machado FB, Yamamoto RE, Zanoli K, Nocchi SR, Novello CR, Schuquel IT, Sakuragui CM, Luftmann H, Ueda-Nakamura T, Nakamura CV, De Mello JC. Evaluation of the antiproliferative activity of the leaves from Arctium lappa by a bioassay-guided fractionation. Molecules. 2012;17(2):1852-9.
    16.
  16. Fierascu RC, Georgiev MI, Fierascu I, Ungureanu C, Avramescu SM, Ortan A, Georgescu MI, Sutan AN, Zanfirescu A, Dinu-Pirvu CE, Velescu BS. Mitodepressive, antioxidant, antifungal and anti-inflammatory effects of wild-growing Romanian native Arctium lappa L. (Asteraceae) and Veronica persica Poiret (Plantaginaceae). Food and Chemical Toxicology. 2018;111:44-52.
    17.
  17. Ojito Betancourt A. Analyse, extraction et récupération de poly-3-hydroxybutyrate présent dans la biomasse. University of Quebec at Montreal Thesis. 2008; 45-55.
    18.
  18. Hayouni EA, Abedrabba M, Bouix M, Hamdi M. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry. 2007;105(3):1126-34.
    19.
  19. Garcia-Vaquero M, Rajauria G, Tiwari B. Conventional extraction techniques: Solvent extraction. In Sustainable Seaweed Technologies 2020; 171-89. Elsevier.
    20.
  20. Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology. 2006;17(6):300-12.
    21.
  21. De Castro ML, Garcıa-Ayuso LE. Soxhlet extraction of solid materials: an outdated technique with a promising innovative future. Analytica Chimica Acta. 1998;369(1-2):1-0.
    22.
  22. Zarnowski R, Suzuki Y. Expedient Soxhlet extraction of resorcinolic lipids from wheat grains. Journal of Food Composition and Analysis. 2004;17(5):649-63.
    23.
  23. Vinatoru M. An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrasonics Sonochemistry. 2001;8(3):303-13.
    24.
  24. Luque-Garcıa JL, De Castro ML. Where is microwave-based analytical equipment for solid sample pre-treatment going? TrAC Trends in Analytical Chemistry. 2003;22(2):90-98.
    25.
  25. Han HJ, Lee JS, Park SA, Ahn JB, Lee HG. Extraction optimization and nanoencapsulation of jujube pulp and seed for enhancing antioxidant activity. Colloids and Surfaces B: Biointerfaces. 2015;130:93-100.
    26.
  26. Prachayasittikul S, Buraparuangsang P, Worachartcheewan A, Isarankura-Na-Ayudhya C, Ruchirawat S, Prachayasittikul V. Antimicrobial and antioxidative activities of bioactive constituents from Hydnophytum formicarum Jack. Molecules. 2008;13(4):904-21.
    27.
  27. Khoshdouni Farahani Z, Khoshdouni Farahani F. Chemical identification of clove (Syzygium aromaticum) extract and essential oil. Applied Biology. 2017; 7(27): 1-7.
    28.
  28. Martino E, Ramaiola I, Urbano M, Bracco F, Collina S. Microwave-assisted extraction of coumarin and related compounds from Melilotus officinalis (L.) Pallas as an alternative to Soxhlet and ultrasound-assisted extraction. Journal of Chromatography A. 2006;1125(2):147-51.
    29.
  29. Mozdastan S, Ebrahimzadeh MA, Khalili M. Comparing the impact of different extraction methods on antioxidant activities of myrtle (Myrtus communis L.). Journal of Mazandaran University of Medical Sciences. 2015;25(127):10-24.
    30.
  30. Rostami H, Gharibzahedi SM. Microwave-assisted extraction of jujube polysaccharide: optimization, purification and functional characterization. Carbohydrate Polymers. 2016;143:100-7.
    31.
  31. Chen Q, Zhao J, Liu M, Cai J, Liu J. Determination of total polyphenols content in green tea using FT-NIR spectroscopy and different PLS algorithms. Journal of Pharmaceutical and Biomedical Analysis. 2008;46(3):568-73.
    32.
  32. Rezaei S, Rezaei K, Haghighi M, Labbafi M. Solvent and solvent to sample ratio as main parameters in the microwave-assisted extraction of polyphenolic compounds from apple pomace. Food Science and Biotechnology. 2013;22(5):1-6.
    33.
  33. Tuncel NB, Yılmaz N. Optimizing the extraction of phenolics and antioxidants from feijoa (Feijoa sellowiana, Myrtaceae). Journal of Food Science and Technology. 2015;52(1):141-50.
    34.
  34. Falleh H, Ksouri R, Lucchessi ME, Abdelly C, Magné C. Ultrasound-assisted extraction: Effect of extraction time and solvent power on the levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Aizoaceae shoots. Tropical Journal of Pharmaceutical Research. 2012;11(2):243-9.
    35.
  35. Khalili M, Ebrahimzadeh MA. A review on antioxidants and some of their common evaluation methods. Journal of Mazandaran University of Medical Sciences. 2015;24(120):188-208.
    36.
  36. Mokrani A, Madani K. Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology. 2016;162,68-76.
    37.
  37. Forouzani M, Askari M, Ebrahimzadeh MA. Evaluation of three methods for the extraction of antioxidants from Cucumis melo L. fruit and leaves. International Journal of Forest, Soil and Erosion (IJFSE). 2013;3(3):95-99.
    38.
  38. Rafińska K, Pomastowski P, Rudnicka J, Krakowska A, Maruśka A, Narkute M, Buszewski B. Effect of solvent and extraction technique on composition and biological activity of Lepidium sativum extracts. Food Chemistry. 2019;289,16-25.
    39.
  39. Nawaz H, Shad MA, Rehman N, Andaleeb H, Ullah N. Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences. 2020;56.
    40.
  40. Rostagno MA, Palma M, Barroso CG. Ultrasound-assisted extraction of soy isoflavones. Journal of Chromatography A. 2003;1012(2):119-28.
    41.
  41. Wang J, Sun B, Cao Y, Tian Y, Li X. Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chemistry. 2008;106(2):804-10.
    42.
  42. Khalili M, Fathi H, Ebrahimzadeh MA. Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods. Pakistan Journal of Pharmaceutical Sciences. 2016;29(3):773-7.
    43.
  43. Dias AL, Sergio CS, Santos P, Barbero GF, Rezende CA, Martínez J. Ultrasound-assisted extraction of bioactive compounds from dedo de moça pepper (Capsicum baccatum L.): Effects on the vegetable matrix and mathematical modeling. Journal of Food Engineering. 2017;198:36-44.
    44.
  44. Predes FS, Ruiz AL, Carvalho JE, Foglio MA, Dolder H. Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts. BMC Complementary and Alternative Medicine. 2011;11(1):1-5.
    45.
  45. Petkova N, Ivanov I, Mihaylova D, Lante A. Effect of pressure liquid extraction and ultrasonic irradiation frequency on inulin, phenolic content and antioxidant activity in burdock (Arctium lappa L.) roots. Acta Scientiarum Polonorum. Hortorum Cultus. 2020;19(3).
    46.
  46. Ferracane R, Graziani G, Gallo M, Fogliano V, Ritieni A. Metabolic profile of the bioactive compounds of burdock (Arctium lappa) seeds, roots and leaves. Journal of Pharmaceutical and Biomedical Analysis. 2010;51(2):399-404.
    47.
  47. Hsu CY. Antioxidant activity of extract from Polygonum aviculare L. Biological Research. 2006;39(2):281-8.
    48.

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]