فرمولاسیون و ارزیابی عصاره میخک بارگذاری شده در هیدروژل کیتوسان بر روی رده سلولی فیبروبلاست موشی
محورهای موضوعی : فصلنامه زیست شناسی جانوری
سئودا شمشاد
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فرشته شریفی
2
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1 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: عصاره میخک, هیدروژل, کیتوسان, زخم‌پوش, ضدباکتریایی,
چکیده مقاله :
آسیبها یا نواقص شدید پوستی می‌تواند عامل بسیاری از مشکلات موجود زنده باشد، که می‌تواند زندگی فرد را تحت تاثیر قرار دهد. وجود فعالیت قابل قبول ضدباکتریایی همراه با زیست‌سازگار و زیستتخریبپذیر بودن نیاز اساس در توسعهی کاربردی زخم‌پوش جهت کاهش یا محدود نمودن سوء مصرف آنتی‌بیوتیک سنتزی است. هدف از مطالعه حاضر طراحی، تهیه و توسعه ساختار هیدروژلی کارآمد بر پایه کیتوسان (CS) حاوی عصاره میخک (CEO) با فعالیت تشدید شده ضد باکتریایی، غیر سمی، و ظرفیت التیام زخم بوده است. ساختار هیدروژل با استفاده از میکروسکوپ الکترونی روبشی (SEM) و آنالیز طیف جذبی (FTIR) مورد مطالعه قرار گرفت. همچنین، سمیت هیدروژل طراحی شده با استفاده از آزمون MTT مورد ارزیابی قرار گرفت. براساس تصاویر SEM افزوده شدن عصاره میخک موجب افزایش اندازه منافذ هیدروژل CS شده است. نتیجه آزمون FTIR تائید کننده حضور و تلفیق عصاره میخک با CS در ساختار هیدروژل بود. تحقیقات ما نشاندهندهی عدم اثر سمی عصاره میخک بارگذاری شده در هیدروژل CS بر روی سلول‌های فیبروبلاست موشی (L929) بود. بعلاوه، هیدروژل طراحی شده فعالیت ضد باکتریایی علیه استافیلوکوکوس ارئوس و اشریشیاکلی بوده است. براساس نتایج بدست آمده، هیدروژل CS/CEO دارای ظرفیت بزرگ کاندید شدن برای کاربرد زخم‌پوش بدون آنتی‌بیوتیک است.
Severe skin damage or defects can cause various problems in living animates, which could affect a patient's life. Developing wound dressings with acceptable antibacterial activity, besides biocompatibility and biodegradability, is essential in developing wound healing applications for decreasing or limiting synthetic antibiotic abuse. The present study aimed to design, prepare, and develop a functional hydrogel structure based on chitosan (CS) containing clove extract (CEO) with enhanced antibacterial activity, non-toxicity, and wound-healing potential. The hydrogel structure was studied using a scanning electron microscope (SEM) and Fourier transform infrared (FTIR). In addition, the cytotoxicity of the designed hydrogel was assayed by the MTT test. Based on the SEM, adding clove extract increased the pore size of the CS hydrogel. FTIR analysis confirmed the presence and mixture of clove extract with CS in the hydrogel structure. Our investigations displayed that the clove extract-loaded CS hydrogel did not have cytotoxic effects on the mouse fibroblast (L929) cells. In addition, the designed hydrogel showed antibacterial activity against Staphylococcus aureus and Escherichia coli. According to the results, CS/CEO hydrogel is a great potential candidate for antibiotic-free wound healing applications.
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