Formulation and Evaluation of Clove Extract-Loaded Chitosan Hydrogel on the Mouse Fibroblast Cell Line
Subject Areas : Journal of Animal Biology
Sevda Shemshad
1
,
Fereshteh Sharifi
2
*
1 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Keywords: Clove extract, Hydrogel, Chitosan, Wound dressing, Antibacterial,
Abstract :
Severe skin damage or defects can cause various problems in living animates, which could affect a patient's life. Developing wound dressings with acceptable antibacterial activity, besides biocompatibility and biodegradability, is essential in developing wound healing applications for decreasing or limiting synthetic antibiotic abuse. The present study aimed to design, prepare, and develop a functional hydrogel structure based on chitosan (CS) containing clove extract (CEO) with enhanced antibacterial activity, non-toxicity, and wound-healing potential. The hydrogel structure was studied using a scanning electron microscope (SEM) and Fourier transform infrared (FTIR). In addition, the cytotoxicity of the designed hydrogel was assayed by the MTT test. Based on the SEM, adding clove extract increased the pore size of the CS hydrogel. FTIR analysis confirmed the presence and mixture of clove extract with CS in the hydrogel structure. Our investigations displayed that the clove extract-loaded CS hydrogel did not have cytotoxic effects on the mouse fibroblast (L929) cells. In addition, the designed hydrogel showed antibacterial activity against Staphylococcus aureus and Escherichia coli. According to the results, CS/CEO hydrogel is a great potential candidate for antibiotic-free wound healing applications.
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