Label-Free Perfect Separation of Michigan Cancer Foundation-7 Cells from White Blood Cells using Both Wall-Induced Lift and Dielectrophoresis Forces
Subject Areas :
meso/micro/nano fabrication
Alireza Alaghemand
1
,
Ali Fattah
2
1 - Electrical Engineering Department, Shahrood University of Technology, Shahrood, Iran
2 - Electrical Engineering Department, Shahrood University of Technology, Shahrood, Iran
Received: 2022-04-23
Accepted : 2022-08-07
Published : 2022-09-01
Keywords:
Microfluidics,
Cancer cells,
Dielectrophoresis,
COMSOL,
separation,
Abstract :
A microchannel device was designed and utilized to separate Michigan Cancer Foundation-7 cells from white blood cells using both wall-induced lift and di-electrophoresis forces. Using COMSOL Multiphysics simulator, the label-free separation process is performed based on the radius size of the particles. The best performance of the structure is obtained with the efficiency of 100% and 99% for cancer and white blood cells separation processes, respectively. For the proposed microfluidic structure, in addition to the highest available efficiency and high separation speed of 12 um/s, the low DC voltage of 6 V is applied, which causes negligible damage to the blood cells.
References:
Reguera, J., et al., Anisotropic Metal Nanoparticles for Surface Enhanced Raman Scattering, Chemical Society Reviews, Vol. 46, No. 13, 2017, pp. 3866-3885.
Anbari, A., et al., Microfluidic Model Porous Media: Fabrication and Applications., Small, Vol. 14, No. 18, 2018, pp. 1703575.
sonnenberg, a., et al., Dielectrophoretic Isolation and Detection of Cancer‐Related Circulating Cell‐Free DNA Biomarkers from Blood and Plasma, Electrophoresis, Vol. 35, No. 12-13, 2014, pp. 1828-1836.
Tae-Hyeong K., et al., FAST: Size-Selective, Clog-Free Isolation of Rare Cancer Cells from Whole Blood at A Liquid–Liquid Interface, Analytical Chemistry, Vol. 89, No. 2, 2016, pp. 1155-1162.
Qin X., et al., Size and Deformability-Based Separation of Circulating Tumor Cells from Castrate Resistant Prostate Cancer Patients Using Resettable Cell Traps, Lab on a Chip, Vol. 15, No. 10, 2015, pp. 2278-2286.
Medema, J. P., Cancer Stem Cells: The Challenges Ahead, Nature cell biology, Vol. 15, No. 4, 2013, pp. 338-344.
Ferlay, J. et al, Cancer Incidence and Mortality Worldwide: Sources, Methods and Major Patterns in Globocan 2012, International Journal of Cancer, Vol. 136, No. 5, 2015, pp. 359-386.
Pommer, Matthew S., et al., Dielectrophoretic Separation of Platelets from Diluted Whole Blood in Microfluidic Channels, Electrophoresis, Vol. 29, No. 6, 2008, pp. 1213-1218.
Han, K. H., Bruno Frazier, A., Lateral-Driven Continuous Dielectrophoretic Microseparators for Blood Cells Suspended in A Highly Conductive Medium, Lab on a Chip, Vol. 8, No. 7, 2008, pp. 1079-1086.
Braschler, T., et al., Continuous Separation of Cells by Balanced Dielectrophoretic Forces at Multiple Frequencies, Lab on a Chip, Vol. 8, No. 2, 2008, pp. 280-286.
Park, S., et al., Continuous Dielectrophoretic Bacterial Separation and Concentration from Physiological Media of High Conductivity, Lab on a Chip, Vol. 11, No. 17, 2011, pp. 2893-2900.
Piacentini, N., et al, Separation of Platelets from Other Blood Cells in Continuous-Flow by Dielectrophoresis Field-Flow-Fractionation, Biomicrofluidics, Vol. 5, No. 3, 2011, pp. 034122.
Patel, S., et al, Microfluidic Separation of Live and Dead Yeast Cells Using Reservoir-Based Dielectrophoresis, Biomicrofluidics, Vol. 6, No. 3, 2012, pp. 034102.
Dash, S., et al., CFD Design of a Microfluidic Device for Continuous Dielectrophoretic Separation of Charged Gold Nanoparticles, Journal of the Taiwan Institute of Chemical Engineers, Vol. 58, 2016, pp. 39-48.
Kale, A., Saurin P., and Xiangchun X., Three-dimensional reservoir-based dielectrophoresis (rDEP) for Enhanced Particle Enrichment, Micromachines, Vol. 9, No. 3, 2018, pp. 123-128.
Zhang X., et al., Numerical Simulation of Circulating Tumor Cell Separation in A Dielectrophoresis Based YY Shaped Microfluidic Device, Separation and Purification Technology, Vol. 255, 2020, pp. 117343.
Kadaksham, J., Singh P., and Aubry. N., Manipulation of Particles Using Dielectrophoresis, Mechanics Research Communications, Vol. 33, No. 1, 2006, pp. 108-122.
Bruus, H., Theoretical Microfluidics, Oxford: Oxford University Press, Vol. 18, 2008.
Brenner, H., The Slow Motion of a Sphere Through a Viscous Fluid Towards a Plane Surface, Chemical Engineering Science, Vol. 16, No. 3-4, 1961, pp. 242-251.
Nerguizian, V., et al., Analytical Solutions and Validation of Electric Field and Dielectrophoretic Force in A Bio‐Microfluidic Channel, Electrophoresis, Vol. 33, No. 3, 2012, pp. 426-435.
Tatsumi, K., et al., Analysis and Measurement of Dielectrophoretic Manipulation of Particles and Lymphocytes Using Rail-Type Electrodes, Medical Engineering & Physics, Vol. 38, No. 1, 2016, pp. 24-32.
Egger, M., Donath E., Electrorotation Measurements of Diamide-Induced Platelet Activation Changes, Biophysical Journal, Vol. 68, No. 1, 1995, pp. 364-372.
Yong Seok, C., Kyung Won, S., Sang Joon, L., Lateral and Cross-Lateral Focusing of Spherical Particles in A Square Microchannel, Lab on a Chip, Vol. 11, No. 3, 2011, pp. 460-465.
Jones, T. B., Liquid Dielectrophoresis on The Microscale, Journal of Electrostatics, Vol. 51, 2001, pp. 290-299.
Sungyoung C., Gwon Lee M., Je Kyun P., Microfluidic Parallel Circuit for Measurement of Hydraulic Resistance, Biomicrofluidics, Vol. 4, No. 3, 2010, pp. 034110.
Myung Gwon L., Sungyoung C., Je Kyun P., Three-Dimensional Hydrodynamic Focusing with A Single Sheath Flow in A Single-Layer Microfluidic Device, Lab on a Chip, Vol. 9, No. 21, 2009, pp. 3155-3160.
Myung Gwon L., et al., Inertial Blood Plasma Separation in A Contraction–Expansion Array Microchannel, Applied Physics Letters, Vol. 98, No. 25, 2011, pp. 253702.
Myung Gwon, L., Sungyoung, and C., Je Kyun, P., Inertial Separation in A Contraction–Expansion Array Microchannel, Journal of Chromatography A, Vol. 1218, No. 27, 2011, pp. 4138-4143.
Myung Gwon, L., et al., Label-Free Cancer Cell Separation from Human Whole Blood Using Inertial Microfluidics at Low Shear Stress, Analytical Chemistry, Vol. 85, No. 13, 2013, pp. 6213-6218.
