بررسی ملکولی ژنهای مقاومت به کینولون (qnr) در سالمونلا تیفی موریوم جدا شده از نمونه‌های غذایی
محورهای موضوعی :
علوم و صنایع غذایی
کیومرث امینی
1
(استادیار، گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد ساوه، ، ساوه، ایران.)
طیبه قاسمی
2
(دانش آموخته کارشناسی ارشد، گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد سیرجان، سیرجان، ایران)
بابک خیرخواه
3
(استادیار، گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد کرمان، کرمان، ایران.)
تاریخ دریافت : 1395/07/21
تاریخ پذیرش : 1395/10/15
تاریخ انتشار : 1396/10/01
کلید واژه:
سالمونلا تیفی موریوم,
&rlm,
Multiplex PCR,
ژنهای &rlm,
qnr&rlm,
&rlm,
,
چکیده مقاله :
سالمونلوز یک بیماری مهم در انسان و گونه های حیوانات است که به وسیله سرووارهای مختلف سالمونلا انتریکا ایجاد می شود. سرووار تیفی موریوم یکی از شایع ترین سرووارها در انسان می باشد. کینولونها و فلوروکینولونها خانواده‎ ‎ای از آنتی بیوتیک های وسیع الطیف هستند که در درمان سالمونلوز مورد استفاده قرار می گیرند. ژنهای qnr جزء عوامل مقاومت کینولونی وابسته به پلاسمید‎ (PMQR) ‎هستند که باعث‎ ‎گسترش مقاومت در باکتریهای خانواده انتروباکتریاسه میشوند‎.‎ هدف از این تحقیق شناسایی ژنهای مقاومت کینولونی qnr در سالمونلا تیفی موریوم جدا شده از نمونه های مواد غذایی بود.در این تحقیق60 نمونه سالمونلای جداسازی شده از مواد غذایی جمع آوری گردید و با استفاده از آزمونهای کشت و بیوشیمیایی تایید شدند. سروتایپینگ نمونه ها با استفاده از آنتی سرم های O و H انجام گرفت. آزمون Multiplex-PCR جهت شناسایی ژنهای qnrA، qnrB و qnrS انجام گرفت.تمامی 60 نمونه با استفاده از آزمونهای کشت و بیوشیمیایی به عنوان سالمونلا تایید شدند. نتایج سروتایپینگ نشان داد هر 60 نمونه مربوط به گروه سرمی B و سرووار تیفی موریوم متعلق بودند. نتایج Multiplex-‎PCR نشان داد 5 نمونه دارای ژن ‎ qnrB ‎و 4 نمونه دارای ژن qnrS مثبت و 1 نمونه دارای ژن qnrA بود‎.‎ نتایج مطالعه حاضر نشان دهنده حضور ژنهای qnr در نمونه های سالمونلا تیفی موریوم جداسازی شده از مواد غذایی می باشد که اهمیت ویژه ای از لحاظ بهداشت عمومی دارا می باشد. جهت جلوگیری از این مقاومت کینولونی، نیاز به برنامه های پایش و مراقبت جهت شناسایی این ژنها می باشد.
چکیده انگلیسی:
Salmonellosis is an important disease in animals and human which is caused by different serovars of Salmonella ‎enterica. Serovars Typhimurium is one of the most prevalent sorvars in humans. Quinolones and floroquinolones ‎are the family of extended-spectrum antibiotics which are used in salmonellosis treatment. Qnr genes are the ‎plasmid-mediated quinolones resistance which leads to resistance in Enterobacteiacea. The aim of this study is ‎identification of quinolone resistance genes qnr in Salmonella Typhimurium isolated from food samples. In this ‎study, 60 Salmonella samples isolated from food was collected and confirmed by culture and biochemical tests. ‎Serotyping was done by O and H antisera. Multiplex-PCR‏ ‏‎ was performed to identify qnrA, qnrB and qnrS genes. ‎All of the 60 isolates were confirmed as Salmonella by culture and biochemical tests. The results of serotyping ‎showed all the 60 isolates were belonged to serogroup B and serovar Typhimurium. Multiplex-PCR test showed 5 ‎samples had the qnrB, 4 had the qnrS and 1 harboured qnrA gene. The results of this study shows the presence of ‎qnr genes in Salmonella Typhimurium isolated from food samples which has a specific public health importance. ‎Therefore, there should be sureveillance and montoring programs to prevent this quinolone resistance.‎
منابع و مأخذ:
Ata, Z., Yibar, A., Arslan, E., Mustak, K., Gunaydin, E. (2014). Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey. Acta Veterinaria Brno. 83: 281–286.
Braden, C.R. (2006). Salmonella enterica serotype Enteritidis and egg; a national apidemic in the united states. Food safety. 43: 512-17.
Cattoir, V., Weill, F.X., Poirel, L., Fabre, L., Soussy, C.J., Nordmann, P. (2007). Prevalence of qnr genes in Salmonella in France. Journal of Antimicrobial Chemotherapy. 59: 751–754.
Cattoir, V., Poirel, L., Rotimi, V., Soussy, C.J., Nordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL producing enterobacterial isolates. Journal of Antimicrobial Chemotherapy. 60: 394e397.
Cortez, A.L.L., Carvalho, A.C.F.B., Ikunob, A.A., Burger, K.P., Vidal-Martins, A.M.C. (2006). Identification of Salmonella spp. Isolates from chicken abattoirs by multiplex-PCR. Research in veterinary science. 81: 340-344.
Egli, T., Koster, W., Meile, L. (2002). Pathogenic microbes in water and food: changes and challenges. FEMS Microbiology Review. 26(2):111–12.
Firoozeh, F., Shahcheraghi, F., ZahraeiSalehi, T., Karimi, V., Aslani, M.M. (2011). Antimicrobial resistance profile and presence of class I integrongs among Salmonella enterica serovars isolated from human clinical specimens in Tehran, Iran. Iranian journal of microbiology. 3(3): 112-17.
Ferrari, R., Galiana, A., Cremades, R., Rodriguez, J.C., Magnani, M., Tognim, M.C.B., et al. (2013). Plasmid-mediated quinolone resistance (PMQR) and mutations in the topoisomerase genes of Salmonella enterica strains from Brazil. Brazilian Journal of Microbiology. 44(2): 657-662.
González, F.A.M. (2013). Association of transferable quinolone resistance determinant qnrB19 with extended-spectrum 𝛽-Lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela. International journal of microbiology. Article ID 628185: 1-7.
Gunell, M., Aulu, L., Jsalava, J., Lukinmaa-Åberg, S., Österblad, M., Ollgren, J., et al. (2014). Cefotaxime-resistant Salmonella enterica in travelers returning from Thailand to Finland. Emerging infectious diseases. 20(7): 1214-1217.
Hamidian, M., Tajbakhsh, M., Peighambari, S.M., Dabiri, H., Shokrzadeh, L., RezaDehbashi, M. (2009). Resistance to floroquinolones in Salmonella isolated from paitients with acute diarrhea referring to Tehran hospitals. Iranian Journal Infection Diseas. 14(44): 51-54.
Jiang, H.X., Song, L., Liu, J., Zhang, X.H., Ren, Y.N., Zhang, W.H., et al. (2014). Multiple transmissible genes encoding fluoroquinolone and third-generation cephalosporin resistance co-located innon-typhoidal Salmonella isolated from food-producinganimals in China. International journal of antimicrobial agent. 43: 242–247.
Jones-Dias, D., Manageiro, V., Francisco, A.P., Martins, A.P., Domingues, G., Louro, D., et al. (2013). Assessing the molecular basis of transferable quinolone resistance in Escherichia coli and Salmonella spp. from food-producing animals and food products. Veterinary Microbiology. 167: 523–531.
Mozafari, A., ForouheshTehrani, H., Niakani, M. (2007). Nalidixic Acid Resistance Rate in Typhoidal and Non-Typhoidal Salmonella Isolated from Hospitalized Patients During One Year Period (2005-2006). Iran University Medical Science.14(56):43-51. [In Persian]
Moghbelli, M., Behnod, V., Ranjbar, R. (2014). A study to determine antibiotic resistance and recognition qnr genes in Shigella strains isolated from patients admitted to Mofid's Children Medical Center, Tehran. Journal of Microbial World. 7(1): 49-57. [In Persian]
Ranjbar, R., Giammanc, G.M., Farshad, S., Owlia, P., Aleo, A., Mammina, C. (2011). Serotypes, antibiotic resistance, and class 1 integrons in Salmonella isolates from pediatric cases of enteritis in Tehran, Iran. Foodborne pathogens and disease. 8: 547-553.
Skov, R., Matuschek, E., Sjolund-Karlsson, M., Ahman, J., Petersen, A., Stegger, M., et al. (2015). Development of a pefloxacin disk diffusion method for detection of fluoroquinolone-resistant Salmonella enterica. Journal of clinical microbiology. 53(11):3411–3417.
Tacket, C.O., Narain, J.P., Sattin, R., Lofgren, J.P., Konigsberg, C.J.R., Rendtorff, R.C. (1984). A multistate outbreak of infections caused by Yersinia enterocolitica transmitted by pasteurized milk. JAMA. 251(4):483–86.
Veldman, K., Pelt, W.V., Mevius, D. (2008). First report of qnr genes in Salmonella in The Netherlands. Journal of Antimicrobial Chemotherapy. 61: 452–463.
Veldman, K.T., Mevius, Dik., Smith, H.E., Dierikx, C.M. (2014). Plasmid mediated quinolone resistance in Enterobacteriaceae. First edition. Utrecht University. The Netherlands, pp. 27-53.
Wang, Y., Yang, B., Wu, Y., Zhang, Z., Meng, X., Xi, M., et al. (2015). Molecular characterization of Salmonella enterica serovar Enteritidis on retail raw poultry in six provinces and two National cities in China. Food Microbiology. 46 : 74-80.
Yang, B., Zhao, H., Cui, S., Wang, Y., Xia, X., Xi, M. (2014). Prevalence and characterization of Salmonella enterica in dried milk-related infant foods in Shaanxi, China. Journal of dairy science. 97(11): 6754–6760.
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Ata, Z., Yibar, A., Arslan, E., Mustak, K., Gunaydin, E. (2014). Plasmid-mediated quinolone resistance in Salmonella serotypes isolated from chicken carcasses in Turkey. Acta Veterinaria Brno. 83: 281–286.
Braden, C.R. (2006). Salmonella enterica serotype Enteritidis and egg; a national apidemic in the united states. Food safety. 43: 512-17.
Cattoir, V., Weill, F.X., Poirel, L., Fabre, L., Soussy, C.J., Nordmann, P. (2007). Prevalence of qnr genes in Salmonella in France. Journal of Antimicrobial Chemotherapy. 59: 751–754.
Cattoir, V., Poirel, L., Rotimi, V., Soussy, C.J., Nordmann, P. (2007). Multiplex PCR for detection of plasmid-mediated quinolone resistance qnr genes in ESBL producing enterobacterial isolates. Journal of Antimicrobial Chemotherapy. 60: 394e397.
Cortez, A.L.L., Carvalho, A.C.F.B., Ikunob, A.A., Burger, K.P., Vidal-Martins, A.M.C. (2006). Identification of Salmonella spp. Isolates from chicken abattoirs by multiplex-PCR. Research in veterinary science. 81: 340-344.
Egli, T., Koster, W., Meile, L. (2002). Pathogenic microbes in water and food: changes and challenges. FEMS Microbiology Review. 26(2):111–12.
Firoozeh, F., Shahcheraghi, F., ZahraeiSalehi, T., Karimi, V., Aslani, M.M. (2011). Antimicrobial resistance profile and presence of class I integrongs among Salmonella enterica serovars isolated from human clinical specimens in Tehran, Iran. Iranian journal of microbiology. 3(3): 112-17.
Ferrari, R., Galiana, A., Cremades, R., Rodriguez, J.C., Magnani, M., Tognim, M.C.B., et al. (2013). Plasmid-mediated quinolone resistance (PMQR) and mutations in the topoisomerase genes of Salmonella enterica strains from Brazil. Brazilian Journal of Microbiology. 44(2): 657-662.
González, F.A.M. (2013). Association of transferable quinolone resistance determinant qnrB19 with extended-spectrum 𝛽-Lactamases in Salmonella Give and Salmonella Heidelberg in Venezuela. International journal of microbiology. Article ID 628185: 1-7.
Gunell, M., Aulu, L., Jsalava, J., Lukinmaa-Åberg, S., Österblad, M., Ollgren, J., et al. (2014). Cefotaxime-resistant Salmonella enterica in travelers returning from Thailand to Finland. Emerging infectious diseases. 20(7): 1214-1217.
Hamidian, M., Tajbakhsh, M., Peighambari, S.M., Dabiri, H., Shokrzadeh, L., RezaDehbashi, M. (2009). Resistance to floroquinolones in Salmonella isolated from paitients with acute diarrhea referring to Tehran hospitals. Iranian Journal Infection Diseas. 14(44): 51-54.
Jiang, H.X., Song, L., Liu, J., Zhang, X.H., Ren, Y.N., Zhang, W.H., et al. (2014). Multiple transmissible genes encoding fluoroquinolone and third-generation cephalosporin resistance co-located innon-typhoidal Salmonella isolated from food-producinganimals in China. International journal of antimicrobial agent. 43: 242–247.
Jones-Dias, D., Manageiro, V., Francisco, A.P., Martins, A.P., Domingues, G., Louro, D., et al. (2013). Assessing the molecular basis of transferable quinolone resistance in Escherichia coli and Salmonella spp. from food-producing animals and food products. Veterinary Microbiology. 167: 523–531.
Mozafari, A., ForouheshTehrani, H., Niakani, M. (2007). Nalidixic Acid Resistance Rate in Typhoidal and Non-Typhoidal Salmonella Isolated from Hospitalized Patients During One Year Period (2005-2006). Iran University Medical Science.14(56):43-51. [In Persian]
Moghbelli, M., Behnod, V., Ranjbar, R. (2014). A study to determine antibiotic resistance and recognition qnr genes in Shigella strains isolated from patients admitted to Mofid's Children Medical Center, Tehran. Journal of Microbial World. 7(1): 49-57. [In Persian]
Ranjbar, R., Giammanc, G.M., Farshad, S., Owlia, P., Aleo, A., Mammina, C. (2011). Serotypes, antibiotic resistance, and class 1 integrons in Salmonella isolates from pediatric cases of enteritis in Tehran, Iran. Foodborne pathogens and disease. 8: 547-553.
Skov, R., Matuschek, E., Sjolund-Karlsson, M., Ahman, J., Petersen, A., Stegger, M., et al. (2015). Development of a pefloxacin disk diffusion method for detection of fluoroquinolone-resistant Salmonella enterica. Journal of clinical microbiology. 53(11):3411–3417.
Tacket, C.O., Narain, J.P., Sattin, R., Lofgren, J.P., Konigsberg, C.J.R., Rendtorff, R.C. (1984). A multistate outbreak of infections caused by Yersinia enterocolitica transmitted by pasteurized milk. JAMA. 251(4):483–86.
Veldman, K., Pelt, W.V., Mevius, D. (2008). First report of qnr genes in Salmonella in The Netherlands. Journal of Antimicrobial Chemotherapy. 61: 452–463.
Veldman, K.T., Mevius, Dik., Smith, H.E., Dierikx, C.M. (2014). Plasmid mediated quinolone resistance in Enterobacteriaceae. First edition. Utrecht University. The Netherlands, pp. 27-53.
Wang, Y., Yang, B., Wu, Y., Zhang, Z., Meng, X., Xi, M., et al. (2015). Molecular characterization of Salmonella enterica serovar Enteritidis on retail raw poultry in six provinces and two National cities in China. Food Microbiology. 46 : 74-80.
Yang, B., Zhao, H., Cui, S., Wang, Y., Xia, X., Xi, M. (2014). Prevalence and characterization of Salmonella enterica in dried milk-related infant foods in Shaanxi, China. Journal of dairy science. 97(11): 6754–6760.
