اثر سطوح مختلف ریزجلبک نانوکلروپسیس (Nannochloropsis oculata) بر عملکرد رشد، فراسنجه‌های خونی و برخی فاکتورهای بیوشیمیایی سرم ماهی گورخری (Danio rerio)
محورهای موضوعی : فیزیولوژی جانوریشیما جعفری 1 , کاوس نظری 2 , محمد اخوان بهابادی 3 , سید پژمان حسینی شکرابی 4 * , مهدی شمسایی 5
1 - گروه شیلات، دانشگاه آزد اسلامی، واحد علوم و تحقیقات، تهران، ایران.
2 - موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
3 - آزمایشگاه بیوتکنولوژی پروتئین دانشکده زیست شناسی دانشگاه تهران
4 - موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی
5 - گروه آموزشی شیلات، دانشکده منایع طبیعی و محیط زیست، واحد علوم و تحقیقات تهران
کلید واژه: نانوکلروپسیس اکولاتا, ماهی گورخری, شاخص رشد, خون شناسی, شاخص‌های بیوشیمیایی سرم,
چکیده مقاله :
مقدمه: یکی از راه‌های اصلی برای افزایش کیفیت خوراک مورد استفاده آبزیان، افزودن انواع ریزجلبک‌ها به عنوان مکمل غذایی به جیره‌های مصرفی می باشد.
هدف: این مطالعه با هدف بررسی اثرات مکمل خوراکی ریزجلبک نانوکلروپسیس بر عملکرد رشد و بقا، برخی فراسنجه‌های خونی و پارامترهای شیمیایی سرم ماهی گورخری انجام شد.
مواد و روش ها: تعداد 315 قطعه ماهی گورخری با وزن اولیه 02/0 ±25/0 گرم در یک طرح آزمایشی تصادفی شامل سه تیمار و 3 تکرار، در 9 آکواریوم تقسیم شدند. بدین منظور جیره‌های آزمایشی با مقادیر صفر (شاهد)، 5 (تیمار 1) و 10 (تیمار 2) درصد جلبک خشک آماده گردید و ماهیان به مدت 54 روز با این جیره ها تغذیه شدند.
نتایج: پس از پایان دوره پرورش، بیشترین مقدار وزن نهایی و افزایش وزن در تیمار 2 مشاهده شد که میزان آن نسبت به تیمار 1 و گروه شاهد دارای اختلاف معنی‌دار بود (05/0>p). همچنین بیشترین نرخ رشد ویژه و کم ترین ضریب تبدیل غذایی نیز در تیمار 2 مشاهده شد. نتایج مربوط به پارامترهای خونی (شامل تعداد گلبول قرمز، تعداد گلبول سفید، شمارش افتراقی لکوسیت‌ها) در بین تیمارها فاقد اختلاف معنی‌دار بود (05/0<p). در بررسی پارامترهای بیوشیمیایی سرم، بیشترین و کمترین میزان پروتئین کل به ترتیب برای تیمار 2 و شاهد ثبت شد.
نتیجه گیری: نتایج این مطالعه نشان داد که افزودن 10 درصد جلبک نانوکلروپسیس اکولاتا به جیره ماهیان، سبب بهبود عملکرد رشد و پارامترهای بیوشیمیایی سرم در ماهیان گورخری می‌گردد.
Introduction:One of the main ways to increase the quality of aquafeed is to add various types of microalgae as a food supplement to the ration.
Aim: In this study, the effects of Nannochloropsis oculata microalgae supplementation was investigated on growth performance, some hematological indices, and serum biochemistry of zebra fish.
Material and methods: For experimental, 315 zebrafish (initial weight 0.25g) were randomly divided into 3 treatments with three replications (35 fish in each aquarium), and they were fed with the produced diets containing 0 (control), 5% (T1), and 10% (T2) of N. oculate for 8 weeks.
Results:At the end of the experiment period, according to the results of the study of growth and survival indices the highest amount of final weight and weight gain was observed in treatment 2, which had a significant difference compared to treatment 1 and the control group (p<0.05).Also, the highest specific growth rate and the lowest food conversion ratio were observed in treatment 2. The analysis result obtained for the blood parameters, including the red blood cell counts, white blood cell counts and leukocyte differential counts had no significant difference between the treatments (p>0.05). In the examination of serum biochemical parameters, the highest and lowest total protein levels were recorded for treatment 2 (421.67 ± 5.51 g/dL) and control (406.67 ± 5.69 g/dL), respectively.
Conclusion: Dietary supplementation of the fish diet with 10% of N. oculate probably improved the growth performance and serum biochemical parameters in zebra fish.
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