In Vitro Callogenesis and Regeneration of Cucumber Plants from Hypocotyl and Cotyledon

Subject Areas : Research On Crop Ecophysiology

ABOUZAR ASADI ¹ (Assistant Professor, Plant Improvement and Seed Production Center and Department of Plant Genetic and Production Engineering, Azad University, Isfahan, Iran.)

Received: 2024-01-27 Accepted : 2024-01-27 Published : 2023-01-01

Keywords: tissue culture, plant growth regulators, BAP, NAA, Keywords: Cucumis sativus,

Abstract :

In Vitro Callogenesis and Regeneration of Cucumber Plants from Hypocotyl and Cotyledon Abouzar Asadi1* 1- Assistant Professor, Plant Improvement and Seed Production Center and Department of Plant Genetic and Production Engineering, Azad University, Isfahan, Iran. *Corresponding email address: [e-mail] Received: 5 November October 2022 Accepted: 18 January 2023 Abstract In vitro culturing of cotyledon and hypocotyl can serve various purposes such as gene transfer and asexual reproduction. This study aimed to regenerate plants from cotyledon and hypocotyl explants of cucumber cultivars, the Iranian landrace Isfahan and Beta Alpha F1 hybrid. The experiment was carried out in the tissue culture laboratory of Azad University of Isfahan in 2023. It employed a factorial completely randomized design with two genotypes and 10 media in the three replicate dishes. The media consisted of MS supplemented with BAP (1, 2, 3, and 4 mg/l) combined with NAA (0.05, 0.1, 0.2, and 0.5 mg/l). The results showed that callus was successfully induced in both cotyledon and hypocotyl explants in both genotypes. The highest percentages of regeneration from cotyledons were observed in 3 mg/l BAP and 0.5 mg/l NAA and 4 mg/l BAP and 0.1 mg/l NAA, with 65% and 60%, respectively, for the Isfahan genotype and in 1 mg/l BAP and 0.2 mg/l NAA and 1 mg/l BAP and 0.5 mg/l NAA, both exhibiting a 60% regeneration rate for Beta Alpha. Furthermore, when Isfahani and Beta-alpha’s hypocotyl explants were cultured in MS supplemented with 2 mg/l BAP and 0.1 mg/l NAA, and 1 mg/L BAP and 0.2 mg/L NAA had 30% and 55% rate of regeneration, respectively. The present findings indicate that induced callus or shoot regeneration depended on genotype and explants. Each genotype requires specific callogenesis and regenerated media to achieve desirable results. Overall, the results suggest that cotyledons in the Isfahan genotype and hypocotyls in the Beta-alpha variety exhibited better regeneration when tested with these plant growth regulators.