بررسی خاصیت ضد میکروبی گیاهان دارویی خرزهره، گوآ، مرزه و خردل سفید بر روی سالمونلا تیفی موریوم
محورهای موضوعی : فصلنامه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذاییبتول حیدری صادق 1 , رضا روحانی 2 , نرجس دهمرده 3 , فرشته جوادیان 4
1 - استادیار طب اورژانس، بیمارستان امیرالمومنین زابل، دانشگاه علوم پزشکی زابل، زابل، ایران
2 - استادیار، گروه جراحی مغز و اعصاب، بیمارستان امیرالمومنین زابل، دانشگاه علوم پزشکی زابل، زابل، ایران
3 - استادیار آناتومی، دانشگاه علوم پزشکی زابل، زابل، ایران
4 - دانشجوی دکتری، گروه زیست شناسی تکوینی سلولی، دانشگاه ملی اراک، اراک، ایران
کلید واژه: سالمونلا تیفی موریوم, فعالیت ضد میکروبی, طیور, گیاهان دارویی,
چکیده مقاله :
سالمونلوز یکی از مهمترین بیماریهای مشترک انسان و دام در سراسر جهان اسـت کـه در انسـان سـبب مسمومیت غذایی، گاستروآنتریت، تب روده ای و باکتریمی میشود. هدف از این مطالعه بررسی خاصیت ضد میکروبی گیاهان دارویی خرزهره، گوآ، مرزه و خردل سفید بر روی سالمونلا تیفی موریوم است.مواد و روش کار:نمونه های سالمونلا تیفی موریوم از مدفوع طیور جداسازی گردید، عصاره گیاهان خرزهره، گوا، مرزه و خردل سفید با استفاده از دستگاه روتاری تهیه گردید و حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی با روش میکرودایلوشن تعیین گردید و همچنین قطر هاله مهاری با روش چاهک به دست آمد.نتایج:نتایج حاصل از بررسی قطر هاله مهاری عصاره های گیاهی نشان داد که بیشترین و کمترین قطر هاله مهاری عصاره گیاه گوا برابر با 8 و 1 میلی متر بوده است و بیشترین و کمترین قطر هاله مهاری عصاره گل خرزهره 15 و 1 میلی متر بوده است. بیشترین و کمترین قطر هاله مهاری عصاره خردل سفید بر روی سالمونلا تیفی موریوم برابر با 12 و 1 میلی متر بوده و همچنین کمترین و بیشترین قطر هاله مهاری عصاره مرزه بر روی سالمونلا تیفی موریوم برابر با 1 و 4 میلی متر بوده است و نتایج نشان داد که کمترین قطر هاله مهاری مربوط به عصاره مرزه بوده است. بحث:نتایج حاصل از این مطالعه نشان داد که گیاهان دارویی اثرات مهار خوبی بر روی سالمونلا تیفی موریوم از خود نشان داده اند.
Salmonellosis is one of the most important common diseases of humans and animals worldwide, which causes food poisoning, gastroenteritis, enteric fever and bacteremia in humans. The purpose of this study is to investigate the antimicrobial properties of the medicinal plants Psidium guajava, Nerium oleander, Sinapis alba, Satureja hortensis on Salmonella Typhimurium.Materials and methods:Salmonella typhimurium samples were isolated from poultry feces, extracts of Psidium guajava, Nerium oleander, Sinapis alba, Satureja hortensis plants were prepared using a rotary device, and the minimum inhibitory concentration and the minimum lethal concentration were determined by the microdilution method, and the diameter of the inhibition zone was determined by the well method.Results:The results of the investigation of the inhibition halo diameter of plant extracts showed that the maximum and minimum inhibition halo diameters of P.guajava plant extract were 8 and 1 mm, and the maximum and minimum inhibition halo diameters of N.oleander flower extract were 15 and 1 mm. The maximum and minimum diameters of the inhibition zone of S.alba extract on Salmonella typhimurium were 12 and 1 mm, and the minimum and maximum diameters of the inhibition zone of S.hortensis extract on Salmonella typhimurium were 1 and 4 mm, and the results showed that that the smallest diameter of the inhibition halo was related to the salty extract.Discuss:The results of this study showed that medicinal plants have shown good inhibitory effects on Salmonella Typhimurium.
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