اثر آنتاگونیستی گونههای تریکودرما علیهFusarium oxysporum f.sp. lycopersici عامل پژمردگی گوجه فرنگی
محورهای موضوعی : گیاه پزشکی
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کلید واژه: Biological control, Tomato, Fusarium wilt, گوجه فرنگی, Trichoderma spp, اثر بازدارندگی, شرایط کنترل شده و گلخانه ای, بیماری پژمردگی, کاربندازیم, گونه های تریکودرما, Mycoparasitism,
چکیده مقاله :
در این تحقیق اثر بازدارندگی پنج گونه از قارچ های تریکودرما شامل T. harzianum, T. koningi, T. longiconis, T. hamatum and T. viride علیه قارچ عامل بیماری پژمردگی فوزاریومی گوجه فرنگی Fusarium oxysporum f.sp. lycopersici در شرایط آزمایشگاهی مورد بررسی قرار گرفت. در بین قارچ های مورد استفاده گونه T. harzianum بیشترین و گونه T. viride کمترین تأثیر را داشتند. در شرایط گلخانه ای مقایسه اثر کاهش بیماری بین خاک و بذر آغشته شده با اسپور گونه T. harzianum انجام گردید. پوشش بذرهای گوجه فرنگی توسط اسپورهای قارچ T. harzianumنتوانست در کاهش بیماری موثر باشد در حالیکه خاک آغشته شده با بیوماس گونه مذکور به میزان 50 گرم در 65 کیلوگرم محیط ذرت- شن به طور معناداری (2/92 درصد) قادر به کنترل بیماری گردید.
In this study, the mycoparasitism inhibitory effects of five Trichoderma species (T. harzianum, T. koningi, T. longiconis, T. hamatum and T. viride) on the growth of the causal agent of tomato Fusarium wilt (Fusarium oxysporum f.sp. lycopersici) were investigated by dual culture in laboratory condition. In this step, the maximum and minimum inhibitory effect was caused by T. harzianum and T. viride. In the greenhouse, the comparison of the efficacy of disease decrease was carried out between soil and seed treatments affected by T. harzianum spores. Results showed that seed treatment did not cause disease decrease but soil treatment caused disease decrease by 92%.
Agrios, G.N., 2000. Significance of plant disease, pp. 25-37, In: Agrios, G.N. (Ed.), Plant Pathology. Academic Press, London.
Alexander, L.J. & Tucker C.M., 1945. Physiological specialization in the tomato wilt fungus Fusarium oxysporum f.sp. lycopersici. Journal of Agricultural Research, 70: 303-313.
Bahatnagar, H., 1986. Influence of environmental condition on antagonistic activity of Trichoderma spp. against Fusarium udum. Indian Journal of Mycology Plant Pathology, 26: 58-63.
Bell, D.K., Well H.D. & Markham C.R., 1982. In vitro antagonism of Trichoderma species against six fungal plant pathogens. Phytopathology, 72: 379-382.
Biswas, K.K., 1992. Screening of isolated of Trichoderma harzianum rifai for their relative bio-control efficiency against a few soil-born plant pathogens, M.Sc. Thesis. Bidhan Chandra Krishi Viswavidyalaya Univ., Mohanpur, West Bengal, India.
Biswas, K.K. & Das N.D., 1999. Biological control of pigeon pea wilt caused by Fusarium udum with Trichoderma spp. Annals of Plant Protection Sciences, 7(1): 46-50.
Cal, A., Larena I., Sabuquillo P. & Melgarejo P., 2004. Biological control of tomato wilts. Recent Research Development in Crop Science, 1: 97-115.
Calvet, C., Pera J. & Bera J.M., 1990. Interaction of Trichoderma spp. with Glomus mossaeae and two wilt pathogenic fungi. Agricultural Ecosystem and Environment, 29: 59-65.
Duncan, D.B., 1955. Multiple range and Multiple F-tests. Biometrics, (2-4): 1-42.
Elad, Y. & Kapat A., 1999. The role of Trichoderma harzianum protease in the biocontrol of Botrytis cinerea. Europian Journal of Plant Pathology, 105: 177-189.
Jee, H. & Kim H.K., 1987. Isolation, identification and antagonism of rizospheric antagonists to cucumber wilt pathogen Fusarium oxysporum f.sp. cucumerinum owen. Journal of Plant Protection, 3: 55-59.
Hayes, W.J. & Laws E.R., 1991. Handbook of Pesticide Toxicology. Vol. 1, Academic Press Inc., India.
Lewis, J.A. & Papavizas G.C., 1987. Application of Trichoderma and Glicladium in alginate pellets for control of Rhizoctonia damping off. Plant Pathology, 34: 438- 446.
Montealegre, J.R., Herrera R., Velasquez J.C., Silva P., Besoain X. & Perez L.M., 2005. Biocontrol of root and crown rot in tomatoes under greenhouse conditions using Trichoderma harzianum and Paenibacillus lentimorbus. Additional effect of solarization. Electronic Journal of Biotechnology, 6: 115-127.
Morsy, E.M., Abdel-Kawi K.A. & Khalil M.N.A., 2009. Efficacy of Trichoderma viride and Bacillus subtilis as biocontrol agents against Fusarium solani on tomato plants. Egyptian Journal of Phytopathology, 37(1): 47-57.
Padmadaya, B. & Reddy H.R., 1996. Screening of Trichoderma spp. against Fusarium oxysporum f.sp. lycopersici causing wilt on tomato. Indian Journal of Mycology Plant Pathology, 26: 288-290.
Papavizas, G.C., 1985. Trichoderma and Gliododium: their biology, ecology and potential of bio-control. Annual Review of Phytopathology, 23: 23-54.
Ramezani, H., 2008. Biological control of root-rot of eggplant caused by Macrophomina phaseolina. American-Eurasian Journal of Agricultural and Environmental Sciences, 4(2): 218-220.
Ramezani, H., 2009. Efficacy of fungal and bacterial bioagents against Fusarium oxysporum f.sp. ciceri on chickpea. Plant Protection Journal, 1: 108-113.
Rick, C.M., 1979. The biology and Taxonomy of the Solanaceae. pp. 667-677, In: Biosystematic studies in Lycopersicon and closely related species of Solanum.
Hawkes, J.G., Lester R.N. & Skelding A.D. (Ed.), Academic Press London.
Rick, C.M., 1976. Tomato. Pp. 263-273, In: Evaluation of Crop Plant, Simmonds, N.W. (Ed.), Longman. New York.
Srinon, W., Chuncheen K., Jirattiwarutkul K., Soytong K. & Kanokmedhakul S., 2006. Efficacies of antagonistic fungi against Fusarium wilt disease of cucumber and tomato and the assay of its enzyme activity. Journal of Agricultural Technology, 2(2):191-201.
Sabalpara, A.N., Priya J., Waghunde R.R. & Pandya J.P., 2009. Antagonism of Trichoderma against sugarcane wilt pathogen (Fusarium moniliformae), American-Eurasian Journal of Sustainable Agriculture, 3(4): 637-638.
Sivan, A. & Chet I., 1986. Biological control of Fusarium species in cotton, wheat and muskmelon by Trichoderma harzianum. Journal of Phytopathology, 116: 39-47.
Sivan, A. & Chet I., 1987. Biological control of Fusarium crown rot of tomato by Trichoderma harzianum under field condition. Plant Disease, 71: 589-592.
Somasekhar, Y.M., Anil Kumar T.B. & Siddaramaiah A.L., 1996. Bio-control of pigeon pea (Cajanus cajan L. Millsp.) wilt (Fusarium udum Butler). Mysore Journal of Agricultural Science, 30: 159-160.
Sumita, R. & Gaikwad S.J., 1995. Efficacy of some fungicides against Fusarium udum in pigeon pea. Journal of Solis and Crops, 5: 137-140.