اثر تمرین هوازی تداومی و بر بیان ژنهای UCP1و PGC1a بافت چربی قهوه ایی در موش های صحرایی نر چاق
محورهای موضوعی : فعالیت بدنی و تندرستی
سروناز علی عسکری
1
,
محمد علی آذربایجانی
2
,
سیروان آتشک
3
,
مقصود پیری
4
,
صالح رحمتی
5
1 - دانشگاه آزاد واحد تهران مرکز
2 - استاد گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران، ایران
3 - گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد مهاباد مهاباد، ایران.
4 - Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch, Tehran, Iran
5 - گروه تربیت بدنی، واحد پردیس، دانشگاه آزاد اسلامی، پردیس، ایران
کلید واژه: بیوژنز میتوکندریایی, ترموژنز, تمرین هوازی,
چکیده مقاله :
هدف:بافت چربی قهوه ای نقش بسیار مهمی در پاتوژنز چاقی دارد. بااین وجود اثر تمرینات هوازی بر این بافت کمتر مورد توجه قرار گرفته است. بر این اساس هدف مطالعه حاضر تعیین اثر اثر تمرین هوازی تداومی و بر بیان ژنهای UCP1و PGC1a بافت چربی قهوه ایی در موش های صحرایی نر چاق بود. روش:در یک مطالعه تجربی 18 سر موش صحرای به عنوان آزمودنی انتخاب و به صورت تصادفی به سه گروه کنترل- تغذیه با غذای نرمال، کنترل -تغذیه با غذای پرچرب و تغذیه با غذای پرچرب و تمرین هوازی تقسیم شدند. گروههای تغذیه با غذای پرچرب به مدت هشت هفته غذای پرچرب دریافت نمودند. تمرین هوازی نیز شامل چهار هفته دویدن روی ترید میل ویژه جوندگان بود. بیان ژن های UCP1و PGC1a بافت چربی قهوه ایی به روش Real Time PCR مورد سنجش قرار گرفت. یافته ها:نتایج نشان داد تغذیه با غذای پرچرب موجب کاهش معنادار بیان ژن های (P=0.002)UCP1و PGC1a(P=0.001) بافت چربی قهوه ایی شد. تمرین هوازی موجب افزایش بیان ژن های UCP1(P=0.040)و PGC1a (P=0.040)بافت چربی قهوه ایی شد. نتیجه گیری: بر اساس یافته های به دست آمده از این مطالعه نتیجه گیری می شود تمرین هوازی به واسطه افزایش بیان زن های مسیر سیگنالینگ بیوژنز میتوکندریایی و ترموژنز بافت چربی قهوه ای ، عوارض متابولیک ناشی از تغذیه با غذای پرچرب را کاهش می دهد. لذا انجام این تمرینات در شرایط تغذیه با غذای پرچرب توصیه می گردد.
Brown adipose tissue plays a very important role in the pathogenesis of obesity. However, the effect of aerobic exercises on this tissue has received less attention. Based on this, the aim of the present study was to determine the effect of continuous aerobic exercise on the expression of UCP1 and PGC1a genes in brown adipose tissue in obese male rats. In an experimental study, 18 male rats were selected as subjects and randomly divided into three groups: control-fed with normal food, control-fed with high-fat food, and fed with high-fat diet and continuous aerobic exercise. The groups fed with high-fat diet received high-fat diet for eight weeks. Continuous aerobic exercise also included four weeks of running on a rodent treadmill. The expression of UCP1 and PGC1a genes in brown adipose tissue was measured by Real Time PCR method. The results showed that feeding with high-fat diet caused a significant decrease in the expression of UCP1 (P=0.002) and PGC1a (P=0.001) genes in brown adipose tissue. Continuous aerobic exercise increased the expression of UCP1 (P=0.040) and PGC1a (P=0.040) genes in brown fat tissue. Based on the findings of this study, it can be concluded that continuous aerobic exercise reduces the metabolic side effects of eating high-fat diet by increasing the expression of mitochondrial biogenesis and thermogenesis of brown adipose tissue. Therefore, it is recommended to perform these exercises while eating high-fat diet.
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