بررسی صفات اسپرم رقیق‌شده قوچ با اسانس آویشن (Thymus vulgaris)، با استفاده از میکروسکوپ فاز‌کنتراست و نرم‌افزار CASA
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
حدیثه قمری منور
1
,
غلامعلی مقدم
2
,
حسین دقیق کیا
3
,
بابک قاسمی پناهی
4
1 - دانشجوی دکترای گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
2 - استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
3 - استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
4 - استادیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران.
تاریخ دریافت : 1401/12/01
تاریخ پذیرش : 1402/05/14
تاریخ انتشار : 1402/02/01
کلید واژه:
آویشن,
اسپرم,
قوچ,
صفات حرکتی,
نرم‌افزار CASA,
چکیده مقاله :
توانایی اسپرم در انجام لقاح به صفات مختلفی ازجمله حرکت، زنده مانی و حجم کافی بستگی دارد. پژوهش حاضر به‌منظور بررسی تاثیر اسانس آویشن بر صفات حرکتی اسپرم قوچ با استفاده از میکروسکوپ فاز کنتراست و نرم‌افزار CASA (Computer Assisted Sperm Analyzer) انجام گرفت. بدین منظور اسپرم گیری از 4 رأس قوچ و هفته ای دوبار انجام شد. ارزیابی اولیه شامل حجم، حرکت موجی و پیش رونده، حرکت درجا و درصد زنده مانی اسپرم ها بود. سپس نمونه های اسپرم با رقیق کننده شاخص استاندارد بر پایه تریس، 20 درصد زرده تخم مرغ، 7 درصد گلیسرول، به علاوه مقادیر صفر، 100، 200 و 400 میکروگرم اسانس آویشن، مخلوط شدند. بعد از رساندن دمای نمونه های اسپرم به 5 درجه سلسیوس در یخچال، به مدت 10 دقیقه در فاصله 4 سانتی متری بالای نیتروژن مایع قرار گرفتند و در نهایت داخل آن غوطه ور شدند. نمونه ها در روزهای صفر و 30 آزمایش، یخ گشایی شدند. در روزهای انجماد و ذوب، تحرک کل و پیش رونده، حرکت درجا، درصد زنده مانی و صفات CASA و مقدار مالون دی آلدئید (malondialdehyde) مورد آزمایش قرار گرفتند. نتایج نشان داد که درصد زنده مانی و تحرک و مقدار MDA در طول دوره آزمایش کاهش معنی داری داشتند (001/0>p) و تیمارهای 100، 200 و 400 میکروگرم آویشن، بیشترین اختلاف معنی دار را نسبت به گروه شاهد در صفات حرکت پیش رونده و کل، زنده‌مانی و CASA به خود اختصاص دادند (001/0>p). در صفت MDA تیمارهای 100 و 200 اختلاف معنی‌داری نسبت به تیمار 400 و شاهد داشتند (001/0>p). نتایج پژوهش حاضر نشان داد که استفاده از اسانس آویشن به عنوان رقیق‌کننده باعث بهبود صفات حرکتی اسپرم و زنده مانی در طول انجماد می شود.
چکیده انگلیسی:
The ability of sperm to perform fertilization depends on various characteristics such as movement, viability, and sufficient volume. The present study was conducted to investigate the effect of Thymus vulgaris essential oil on the motility characteristics of ram sperm using phase-contrast microscope and CASA (Computer Assisted Sperm Analyzer) software. For this purpose, semen was collected twice a week from four rams. Initial evaluations included volume, wave motility, progressive motility, non-progressive motility and survival percentage of sperms. Then sperm samples were mixed with standard index Tris-based diluent, 20% egg yolk, 7% glycerol, plus 0, 100, 200 and 400 µg of Thymus vulgaris essential oil per ml. After bringing the temperature of the sperm samples to 5 degrees Celsius in the refrigerator, they were placed at 4 cm above liquid nitrogen for 10 minutes and finally immersed in it. The samples were thawed on days 0 and 30 of the experiment. Traits which were investigated during freezing and thawing days included total and progressive motility, non-progressive motility, viability percentage, MDA (Malondialdehyde) and CASA characteristics. The results showed that the percentage of viability and mobility decreased significantly during the experiment (p<0.001). The treatments of 100, 200 and 400 µg Thymus vulgaris essential oil had the most significant percentages compared to the control group in the characteristics of progressive and total motility, viability and CASA (p<0.001). Malondialdehyde had significantly decreased in the 100 and 200 treatments compared to the 400 and control treatments (p<0.001). The results of the present study showed that diluents containing Thymus vulgaris essential oil improve sperm motility and viability during freezing.
منابع و مأخذ:
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Borges, J., França, G., Cruz, M., Lanza, R., Nascimento, A. and Lessa, M. (2017). Aerobic exercise training induces superior cardioprotection following myocardial ischemia reperfusion injury than a single aerobic exercise session in rats. Motriz: Revista de Educação Física, 23(Special Issue): 1-5.
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Abreu, C., Cardozo, L., Stockler-Pinto, M.B., Esgalhado, M., Barboza, J.E., Frauches, R., et al. (2017). Does resistance exercise performed during dialysis modulate Nrf2 and NF-κB in patients with chronic kidney disease? Life Sciences, 188(1): 192-197.
Adel, M., Rami, M., Shaheen, D., Monir, R., Basheir, M., Nabawy, A., et al. (2020). L-Carnitine protects kidney against ischemia reperfusion injury via suppression of expression of tubular kidney injury molecule (Kim-1) and Wnt proteins (Beta-Catenin). Bulletin of Egyptian Society for Physiological Sciences, 40(1): 32-45.
Amaral, L.S., Souza, C.S., Lima, H. and Soares, T. (2020). Influence of exercise training on diabetic kidney disease: A brief physiological approach. Experimental Biology and Medicine, 245(13): 1142-1154.
Amouoghli Tabrizi, B. and Mohajeri, D. (2015). Study of the protective effects of crocin on experimental ischemia-reperfusion injury of the liver in rats. Veterinary Clinical Pathology, 9(2): 103-116. [In Persian]
Aydogdu, N., Atmaca, G., Yalcin, O., Taskiran, R., Tastekin, E. and Kaymak, K. (2006). Protective effects of L‐Carnitine on Myoglobinuric acute renal failure in Rats. Clinical and Experimental Pharmacology and Physiology, 33(1‐2): 119-124.
Bhalodia, Y., Kanzariya, N., Patel, R., Patel, N., Vaghasiya, J., Jivani, N., et al. (2009). Renoprotective activity of benincasa cerifera fruit extract on ischemia/reperfusion-induced renal damage in rat. Iranian Journal of Kidney Diseases, 3(2): 80-85.
Borges, J., França, G., Cruz, M., Lanza, R., Nascimento, A. and Lessa, M. (2017). Aerobic exercise training induces superior cardioprotection following myocardial ischemia reperfusion injury than a single aerobic exercise session in rats. Motriz: Revista de Educação Física, 23(Special Issue): 1-5.
Chen, Y., Cheng, C., Liu, C., Sue, Y., Chen, T., Hsu, Y., et al. (2021). Combined protective effects of oligo-fucoidan, fucoxanthin, and L-carnitine on the kidneys of chronic kidney disease mice. European Journal of Pharmacology, 892(5): 173-708.
Cipryan, L. (2018). The effect of fitness level on cardiac autonomic regulation, IL-6, total antioxidant capacity, and muscle damage responses to a single bout of high-intensity interval training. Journal of Sport and Health Science, 7(3): 363-371.
Coelho, B., Rocha, L., Scarabelot, K., Scheffer, D., Ronsani, M., Silveira, P., et al. (2010). Physical exercise prevents the exacerbation of oxidative stress parameters in chronic kidney disease. Journal of Renal Nutrition, 20(3): 169-175.
de Lima, W.V., Visona, I., Schor, N. and Almeida, W.S. (2019). Preconditioning by aerobic exercise reduces acute ischemic renal injury in rats. Physiological Reports, 7(14): 14-176.
de Sousa, C.V., Sales, M., Rosa, T.S., Lewis, J.E., de Andrade, R. and Simões, H. (2017). The antioxidant effect of exercise: a systematic review and meta-analysis. Sports Medicine, 47(2): 277-293.
Ding, Y.H., Mrizek, M., Lai, Q., Wu, Y., Reyes, R., Li, J., et al. (2006). Exercise preconditioning reduces brain damage and inhibits TNF-α receptor expression after hypoxia/reoxygenation: an in vivo and in vitro study. Current Neurovascular Research, 3(4): 263-271.
Dokmeci, D., Inan, M., Basaran, U., Yalcin, O., Aydogdu, N., Turan, F., et al. (2007). Protective effect of L‐carnitine on testicular ischaemia–reperfusion injury in rats. Cell Biochemistry and Function: Cellular Biochemistry and its Modulation by Active Agents or Disease, 25(6): 611-618.
Dostar Y. (2017). Protective study of regular aerobic exercise on heart ischemia-reperfusion injury in rats. Comparative Pathobiology 14(4): 2333-2344. [In Persian]
Estrela, G., Wasinski, F., Batista, R., Hiyane, M., Felizardo, R., Cunha, F., et al. (2017). Caloric restriction is more efficient than physical exercise to protect from cisplatin nephrotoxicity via PPAR-alpha activation. Frontiers in Physiology, 2(8): 116.
Faleiros, C., Francescato, H., Papoti, M., Chaves, L., Silva, C., Costa, R., et al. (2017). Effects of previous physical training on Adriamycin nephropathy and its relationship with endothelial lesions and angiogenesis in the renal cortex. Life Sciences, 169(15): 43-51.
Fathizadeh, H., Milajerdi, A., Reiner, Ž., Amirani, E., Asemi, Z., Mansournia, M., et al. (2020). The effects of L-carnitine supplementation on indicators of inflammation and oxidative stress: a systematic review and meta-analysis of randomized controlled trials. Journal of Diabetes & Metabolic Disorders, 19(2): 1879-1894. [In Persian]
Ferreira, G. and McKenna, M. (2017). L-Carnitine and acetyl-L-carnitine roles and neuroprotection in developing brain. Neurochemical Research, 42(6): 1661-1675.
Francescato, H.D., Almeida, L.F., Reis, N.G., Faleiros, C.M., Papoti, M., Costa, R., et al. (2018). Previous exercise effects in cisplatin-induced renal lesions in rats. Kidney and Blood Pressure Research, 43(2): 582-593.
Giudetti, A., Stanca, E., Siculella, L., Gnoni, G. and Damiano, F. (2016). Nutritional and hormonal regulation of citrate and carnitine/acylcarnitine transporters: two mitochondrial carriers involved in fatty acid metabolism. International Journal of Molecular Sciences, 17(6): 817.
Gomez-Cabrera, M.C., Salvador-Pascual, A., Cabo, H., Ferrando, B., and Viña, J. (2015). Redox modulation of mitochondriogenesis in exercise. Does antioxidant supplementation blunt the benefits of exercise training? Free Radical Biology and Medicine, 86(1): 37-46.
Hong, X., Zhao, X., Wang, G., Zhang, Z., Pei, H. and Liu, Z. (2017). Luteolin treatment protects against renal ischemia-reperfusion injury in rats. Mediators of inflammation, 2017 (Article ID; 9783893): 1-10.
Kart, A., Yapar, K., Karapehlivan, M., Tunca, R., Ogun, M. and Citil, M. (2006). Effects of L-carnitine on kidney histopathology, plasma and tissue total sialic acid, malondialdehyde and glutathione concentrations in response to gentamicin administration in Balb/C mice. Revue de Médecine Vétérinaire, 157(4): 179-184.
Kavouras, S.A., Panagiotakos, D.B., Pitsavos, C., Chrysohoou, C., Arnaoutis, G., Skoumas, Y., et al. (2010). Physical activity and adherence to Mediterranean diet increase total antioxidant capacity: The ATTICA study. Cardiology Research and Practice, 10(20): 21-28.
Koohpeyma, F., Siri, M., Allahyari, S., Mahmoodi, M., Saki, F. and Dastghaib, S. (2021). The effects of L-carnitine on renal function and gene expression of caspase-9 and Bcl-2 in monosodium glutamate‐induced rats. BMC Nephrology, 22(1): 1-11. [In Persian]
Lee, J., Park, S. and Kim, W. (2013). Exercise preconditioning reduces acute ischemic renal injury in Hsp70. 1 knockout mouse. Histology and Histopathology, 28(9): 1223-1232.
Liu, H.., Kao, H. and Wu, C. (2019). Exercise training upregulates SIRT1 to attenuate inflammation and metabolic dysfunction in kidney and liver of diabetic db/db mice. Nutrition & Metabolism, 16(1): 1-10.
Malek, M. and Nematbakhsh, M. (2015). Renal ischemia/reperfusion injury; from pathophysiology to treatment. Journal of Renal Injury Prevention, 4(2): 20-27. [In Persian]
Mas-Bargues, C., Escrivá, C., Dromant, M., Borrás, C. and Viña, J. (2021). Lipid peroxidation as measured by chromatographic determination of malondialdehyde. Human plasma reference values in health and disease. Archives of Biochemistry and Biophysics, 709(9): 10-16.
Moecke, D., Martins, G., Garlet, T., Bonorino, K., Luciani, M., Bion, M., et al. (2022). Aerobic Exercise Attenuates Kidney Injury, Improves Physical Performance, and Increases Antioxidant Defenses in Lungs of Adenine-Induced Chronic Kidney Disease Mice. Inflammation, 45(5): 1895-1910.
Mohajeri, D., Mousavi, G. and Mohammadi, P. (2013). Histopathological study of the effect of alcoholic extract of turnip root on kidney ischemia-reperfusion injury in rats. Veterinary Clinical Pathology, 6(2): 1549-1559. [In Persian]
Moraes, C., Marinho, S., Da Nobrega, A., de Oliveira Bessa, B., Jacobson, L., Stockler-Pinto, M., et al. (2014). Resistance exercise: a strategy to attenuate inflammation and protein-energy wasting in hemodialysis patients? International Urology and Nephrology, 46(8): 1655-1662.
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