بررسی امکان بهره‌گیری از روش بیورزونانس در تشخیص گربه‌های مبتلا به FIP
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی
فرنوش فارسیجانی
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شهاب الدین صافی
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حامد بهشتی
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1 - گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه پاتوبیولوژی، دانشکده علوم تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
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کلید واژه: پریتونیت عفونی گربه (FIP), بیورزونانس, واکنش زنجیره‌ای پلیمراز (PCR) , کرونا ویروس,
چکیده مقاله :
مقدمه: عفونت کرونا ویروسی یکی از شایع ترین بیماری‌های ویروسی در سراسر دنیا است و تشخیص سریع و دقیق آن بسیار چالش برانگیز است. تکنیک‌های مختلفی برای تشخیص آن ایجاد شده است که با محدودیت‌هایی همراه هستند. اما بررسی نتایج چند روش تشخیصی در کنار هم با در نظر گرفتن علائم بالینی میتواند به تشخیص دقیق‌تر با اطمینان بیشتر ما را نزدیک کند.
هدف: پژوهش حاضر به منظور ارزیابی کارایی روش بیورزونانس و استفاده از فرکانس نوسانی خاص (SMF) در مقایسه با سایر روش‌های تشخیصی معمول (الکتروفورز پروتئین‌های سرم و واکنش زنجیره‌ای پلیمراز (PCR) برای تشخیص کرونا ویروس در گربه‌های مشکوک به FIP انجام شد.
روش کار: نمونه خون از 30 گربه بیمار مشکوک به FIP بر اساس علائم بالینی جمع آوری شد. برای هر نمونه آزمایشات PCR و SMF انجام شد. حساسیت، ویژگی و تطابق بین آزمون ها با استفاده از استاندارد طلایی (ترکیب نتایج PCR، و بیورزونانس) و همچنین روش ضریب کاپا محاسبه شد.
یافته‌ها: حساسیت و ویژگی، PCR و SMF برای تشخیص FIP به ترتیب 70.6% ، 72.7% ،100% و 81% بود.
نتیجه‌گری: با توجه به یافته‌های به دست آمده از مطالعه حاضر، SMF نسبت به روش‌های تشخیصی دیگر، دارای حساسیت و ویژگی بالاتری است که میتواند درتشخیص FIP موثر و کارآمد تلقی شود.
Introduction: Coronavirus infection is one of the most prevalent viral diseases worldwide, and its rapid and accurate diagnosis is highly challenging. Various diagnostic techniques have been developed, but they come with limitations. However, evaluating the results of multiple diagnostic methods alongside clinical symptoms can provide a more accurate and reliable diagnosis.
Objective: This study aimed to evaluate the efficiency of the Bioresonance method and the use of specific oscillatory frequency (SMF) in comparison with conventional diagnostic methods (serum protein electrophoresis and polymerase chain reaction [PCR]) for the diagnosis of coronavirus in cats suspected of having Feline Infectious Peritonitis (FIP).
Methods: Blood samples were collected from 30 clinically suspected FIP-infected cats. For each sample, PCR and SMF tests were performed. Sensitivity, specificity, and agreement between tests were calculated using the gold standard (a combination of PCR and Bioresonance results) and the kappa coefficient method.
Results: The sensitivity and specificity of PCR and SMF for FIP diagnosis were 70.6% and 72.7% for PCR and 100% and 81% for SMF, respectively.
Conclusion: Based on the findings of this study, SMF showed higher sensitivity and specificity compared to other diagnostic methods and can be considered an effective and reliable tool for FIP diagnosis.
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