ردیابی مولکولی اورنیتوباکتریوم رینوتراکئال (ORT) و ویروس بیماری نیوکاسل در شترمرغ‌های استان اصفهان
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
عباس علی شعبانی
1
,
مجید غلامی آهنگران
2
,
حسن ممتاز
3
1 - دانش‌آموخته دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
2 - دانشیار بخش بیماری‌های طیور، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
3 - استاد بخش میکروب‌شناسی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
تاریخ دریافت : 1394/12/13
تاریخ پذیرش : 1396/05/04
تاریخ انتشار : 1396/04/01
کلید واژه:
اصفهان,
PCR,
شترمرغ,
اورنیتوباکتریوم رینوتراکئال,
ویروس نیوکاسل,
چکیده مقاله :
با هدف شناسایی باکتری اورنیتوباکتریوم رینوتراکئال (ORT) و ویروس بیماری نیوکاسل در تلفات حاد شترمرغ، 40 نمونه نای از 22 گله شترمرغ واجد تلفات از نقاط مختلف استان اصفهان با ثبت تاریخچه جمع‌آوری شد. پس از استخراج RNA و DNA از بافت نای با کیت ترانس کریپتاز معکوس cDNA تهیه شد. DNA استخراج شده با پرایمرهای اختصاصی جهت تکثیر ژن 16srRNA باکتری ORT مورد ارزیابی قرار گرفت و cDNA تهیه شده با پرایمرهای اختصاصی ژن M نیوکاسل تکثیر شد. ژن 16srRNA در کنترل مثبت به‌خوبی تکثیر شد و قطعه 784 جفت بازی به دست آمد، اما در هیچ کدام از نمونه‌های اخذشده، قطعه مورد نظر تکثیر نشد. علاوه بر آن، در 24 نمونه از 40 نمونه جمع‌آوری شده (60 درصد)، قطعه 1097 جفت بازی ژن M ویروس نیوکاسل تکثیر شد. از مجموع 22 گله مورد بررسی با مرگ حاد، 15 گله یا به‌عبارتی 18/68درصد گله‌ها آلوده به ویروس نیوکاسل بودند. با توجه به نتایج این مطالعه می‌توان بیان داشت که در حال حاضر آلودگی با ORT در گله‌های شترمرغ در استان اصفهان منتفی است، اما ویروس نیوکاسل سهم بالایی در ایجاد تلفات حاد در گله‌های شترمرغ دارد.
چکیده انگلیسی:
With the purpose of identifying Ornithobacterium rhinotracheale (ORT) and Newcastle disease virus in acute death of ostriches, 40 tracheal samples from 22 ostrich farms with acute mortality were collected from all over the Isfahan province with the history recorded. After RNA and DNA extraction from tracheal tissue, the cDNA was prepared using reverse transcriptase kit. The extracted DNA was evaluated for amplification of 16srRNA gene of ORT using specific primers and cDNA was amplified by Newcastle disease specific primers based on M gene. The 16srRNA gene was amplified in positive control and a 784 bp fragment was reached but it was not amplified in any of the collected samples. Furthermore, in 24 of the 40 collected samples (60%) a 1097 bp fragment of the M gene of Newcastle disease virus was amplified. From a total of 22 evaluated flocks with acute mortality, 22 flocks (68.18%) were infected with Newcastle disease virus. By considering the results of this study, currently there is no infection with ORT in ostrich flocks of Isfahan province, but Newcastle disease virus has the main role in acute deaths in ostrich farms.
منابع و مأخذ:
Alexander, D.J. (2000). Newcastle disease in ostriches (Struthio camelus)- a review. Avian Pathology, 29(2): 95-100.
Anonymous. (1995). Ornithobacterium rhinotrachele in ostrich. Khangela Quarterly Report on Aniaml Diseases Surveillance, South Africa, pp: 11.
Cadman, H.F., Kelly, P.J., de Angelis, N.D., Rohde, C., Collins, N. and Zulu, T. (1997). Comparison of enzyme‐linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus). Avian Pathology, 26(2): 357-363.
Chin, R.P., van Empel, P.C.M. and Hafez, H.M. (2013). Ornithobacterium rhinotracheale Infection. In: Disease of Poultry. Swayne, D.E., Glisson, J.R., McDougald, R., Nolan, L.K, Suarez, D.L. and Nair, V.L. editors. 13th ed., USA: W.B. Publishing, Massachusetts, pp: 830-840.
Eroksuz, H., Ozbey, G., Cevik, A., Gencer Tarakci, B. and Balik, D.T. (2006). Immuno-histochemical, pathological, enzyme linked immunosorbent assay and polymerase chain reaction analysis of experimental Ornithobacterium rhinotracheale infection in quails (Coturnix coturnix japonica). Revue de Médicinae Véterinaria, 157(4): 197-202.
Ghalyanchi-Langeroudi, A., Hosseini, H., Madadgar, O., Karimi, V., Shahraeini, A. and Ghafari, M.M. (2011). Sequence analysis of fusion gene of Newcastle disease viruses isolated from ostrich (Struthio camelus) in Iran, 2012. Iranian Journal of Virology, 5(3): 12-17.
Ghiamirad, M., Pourbakhsh, A., Keyvanfar, H., Momayaz, R., Charkhkar, S. and Ashtari, A. (2010). Isolation and characterization of Newcastle disease virus from ostriches in Iran. African Journal of Microbiology Research, 4(23): 2492-2497.
Hafez, M.H. and Michael L. (2010). Ornithobacterium rhinotracheale in Nestling Falcons. Avian Diseases Digest, 5(1): e51-e52.
Huchzermeyer, F.W. and Gerdes, G.H. (1993). Newcastle disease virus isolated from ostriches in South Africa. Journal of the South African Veterinary Association, 46: 140.
Ley, E.C., Morishita, T.Y., Harr, B.S., Mohan R. and Brisker T. (2000). Serologic survey of slaughter-age ostriches (Struthio camelus) for antibodies to selected avian pathogens. Avian Diseases, 44(4): 989-992.
Miller, P.J. and Koch, G. (2013). Newcastle disease. In: Disease of Poultry. Swayne, D.E., Glisson, J.R., McDougald, R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., USA: W.B. Publishing, Massachusetts, pp: 92-120.
Mirzaei, S., Hassanzadeh, M., Bozorgmehrifard, M.H. and Banani, M. (2011). Isolation and characterization of Ornithobacterium rhinotracheale in the commercial turkey, quail flocks and domestic pigeons by bacteriological and molecular methods. Archives of Razi Institute, 66(2): 121-126.
Moreno, B., Chacón, G., Villa, A., Fernández, A., Vela, A.I., Fernández-Garayzábal, J.F., Ferré, S. and Gracia E. (2009). Nervous signs associated with otitis and cranial osteomyelitis and withOrnithobacterium rhinotracheale infection in red-legged partridges (Alectoris rufa). Avian Pathology, 38(5): 341-347.
Pagés, A., Foix, A., March, R. and Artigas, C. (1995). Estudio bacteriológico de un agente asociado a problemas respiratorios en aves de producción: Ornithobacterium rhinotracheale. Medicina Veterinaria, 12: 585-588.
Samberg, Y., Hadash, D.U., Perelman, B. and Meroz, M. (1989). Newcastle disease in ostriches (Struthio camelus): field case and experimental infection. Avian Pathology, 18(2): 221-226.
Shanawany, M.M. (1999). Ostrich production systems. Parts 1-2. Food and Agriculture Organization, pp: 256.
Vandamme, P., Segers, P., Vancanneyt, M., Van hofe, K., Mutters, R. and Hommez, J. (1994). Ornithobacterium rhinotracheale gen. nov. isolated from the avian respiratory tract. International Journal of Systemic Bacteriology, 44: 24-37.
Van Empel, P. and Hafez, H.M. (1999). Ornithobacterium rhinotracheale: a review. Avian Pathology, 28: 217-227.
Wehmann, E., Herczeg, J., Ballagi-Pordány, A. and Lomniczi, B. (1997). Rapid identification of Newcastle disease virus vaccine strains LaSota and B-1 by restriction site analysis of their matrix gene. Vaccine, 15(12-13): 1430-1433.
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Alexander, D.J. (2000). Newcastle disease in ostriches (Struthio camelus)- a review. Avian Pathology, 29(2): 95-100.
Anonymous. (1995). Ornithobacterium rhinotrachele in ostrich. Khangela Quarterly Report on Aniaml Diseases Surveillance, South Africa, pp: 11.
Cadman, H.F., Kelly, P.J., de Angelis, N.D., Rohde, C., Collins, N. and Zulu, T. (1997). Comparison of enzyme‐linked immunosorbent assay and haemagglutination inhibition test for the detection of antibodies against Newcastle disease virus in ostriches (Struthio camelus). Avian Pathology, 26(2): 357-363.
Chin, R.P., van Empel, P.C.M. and Hafez, H.M. (2013). Ornithobacterium rhinotracheale Infection. In: Disease of Poultry. Swayne, D.E., Glisson, J.R., McDougald, R., Nolan, L.K, Suarez, D.L. and Nair, V.L. editors. 13th ed., USA: W.B. Publishing, Massachusetts, pp: 830-840.
Eroksuz, H., Ozbey, G., Cevik, A., Gencer Tarakci, B. and Balik, D.T. (2006). Immuno-histochemical, pathological, enzyme linked immunosorbent assay and polymerase chain reaction analysis of experimental Ornithobacterium rhinotracheale infection in quails (Coturnix coturnix japonica). Revue de Médicinae Véterinaria, 157(4): 197-202.
Ghalyanchi-Langeroudi, A., Hosseini, H., Madadgar, O., Karimi, V., Shahraeini, A. and Ghafari, M.M. (2011). Sequence analysis of fusion gene of Newcastle disease viruses isolated from ostrich (Struthio camelus) in Iran, 2012. Iranian Journal of Virology, 5(3): 12-17.
Ghiamirad, M., Pourbakhsh, A., Keyvanfar, H., Momayaz, R., Charkhkar, S. and Ashtari, A. (2010). Isolation and characterization of Newcastle disease virus from ostriches in Iran. African Journal of Microbiology Research, 4(23): 2492-2497.
Hafez, M.H. and Michael L. (2010). Ornithobacterium rhinotracheale in Nestling Falcons. Avian Diseases Digest, 5(1): e51-e52.
Huchzermeyer, F.W. and Gerdes, G.H. (1993). Newcastle disease virus isolated from ostriches in South Africa. Journal of the South African Veterinary Association, 46: 140.
Ley, E.C., Morishita, T.Y., Harr, B.S., Mohan R. and Brisker T. (2000). Serologic survey of slaughter-age ostriches (Struthio camelus) for antibodies to selected avian pathogens. Avian Diseases, 44(4): 989-992.
Miller, P.J. and Koch, G. (2013). Newcastle disease. In: Disease of Poultry. Swayne, D.E., Glisson, J.R., McDougald, R., Nolan, L.K., Suarez, D.L. and Nair, V.L. editors. 13th ed., USA: W.B. Publishing, Massachusetts, pp: 92-120.
Mirzaei, S., Hassanzadeh, M., Bozorgmehrifard, M.H. and Banani, M. (2011). Isolation and characterization of Ornithobacterium rhinotracheale in the commercial turkey, quail flocks and domestic pigeons by bacteriological and molecular methods. Archives of Razi Institute, 66(2): 121-126.
Moreno, B., Chacón, G., Villa, A., Fernández, A., Vela, A.I., Fernández-Garayzábal, J.F., Ferré, S. and Gracia E. (2009). Nervous signs associated with otitis and cranial osteomyelitis and withOrnithobacterium rhinotracheale infection in red-legged partridges (Alectoris rufa). Avian Pathology, 38(5): 341-347.
Pagés, A., Foix, A., March, R. and Artigas, C. (1995). Estudio bacteriológico de un agente asociado a problemas respiratorios en aves de producción: Ornithobacterium rhinotracheale. Medicina Veterinaria, 12: 585-588.
Samberg, Y., Hadash, D.U., Perelman, B. and Meroz, M. (1989). Newcastle disease in ostriches (Struthio camelus): field case and experimental infection. Avian Pathology, 18(2): 221-226.
Shanawany, M.M. (1999). Ostrich production systems. Parts 1-2. Food and Agriculture Organization, pp: 256.
Vandamme, P., Segers, P., Vancanneyt, M., Van hofe, K., Mutters, R. and Hommez, J. (1994). Ornithobacterium rhinotracheale gen. nov. isolated from the avian respiratory tract. International Journal of Systemic Bacteriology, 44: 24-37.
Van Empel, P. and Hafez, H.M. (1999). Ornithobacterium rhinotracheale: a review. Avian Pathology, 28: 217-227.
Wehmann, E., Herczeg, J., Ballagi-Pordány, A. and Lomniczi, B. (1997). Rapid identification of Newcastle disease virus vaccine strains LaSota and B-1 by restriction site analysis of their matrix gene. Vaccine, 15(12-13): 1430-1433.
