Spectroscopic studies on the interaction of Fe3O4@CaAl LDH@Lamivudine with the calf thymus DNA

Subject Areas : Journal of Nanoanalysis

Nahid Shahabadi ¹ , Mahtab Razlansari ² , Avat (Arman) Taherpour ³

1 - Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran
2 - Inorganic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran
3 - Organic Chemistry Department, Faculty of Chemistry, Razi University, Kermanshah, Iran

Received: 2018-04-11 Accepted : 2018-08-11 Published : 2018-10-01

Keywords: Lamivudine, Fe3O4, CT-DNA, Layered Double Hydroxide, Groove Binding,

Abstract :

In this study, we were synthesized Fe3O4@LDH@Lamivudine and characterized by FT-IR spectroscopy,XRD and TEM. The interaction of this nanoparticle with CT-DNA was investigated by viscosity, circulardichroism (CD), Uv-Visible and fluorescence spectroscopy. Among all nanocarriers which applied as drugdelivery vectors, layered double hydroxides (LDHs) with exchangeable anions in the positive brucitelikeinterlayers have been attracting much attentions in the field of cellular delivery of anionic drug andother bio-functional molecules, due to their low toxicity, biocompatibility, high stability, pH dependentsolubility and enhanced cellular uptake behavior compared with the conventional drug carriers. UVVisibleabsorption studies indicated hyperchromism with the binding constant of 4.9×103 M-1. In thefluorimetric investigation, this nanocomposite can bind to DNA and creates a new non-fluorescenceadduct. The thermodynamic parameters (ΔHand nanocomposite is hydrogen bond and Vander-Waals force. The process of binding was spontaneous,in which Gibbs free energy change (ΔG) was negative. Furthermore, viscosity measurements did not showany changes by increasing the amount of the mentioned nanocomposite. In Circular dichroism, bothpositive and negative bands illustrate little changes, which imply a non-intercalative mode of binding. Theexperimental results demonstrated that Fe3O4@LDH@Lamivudine interact with DNA by groove bindingmode. As an evidenced, increasing the fluorescence intensity of Hoechst–DNA solutions in the presence ofdifferent amounts of Fe3O4@LDH@Lamivudine nanoparticles are able to displace the Hoechst molecules,which was grooved into DNA completely as to indicate groove binding mode.

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