In vitro Cytotoxicity Effect of Lactobacillus casei on Kyse-30 Human Esophageal Cancer Cells

Subject Areas : Journal of Chemical Health Risks

Malihe Hashemi Khah ¹ , Nazila Arbab Soleymani ² , Mohammad Mahdi Forghanifard ³ , Omid Gholami ⁴ , Saba Taheri ⁵

1 - Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
4 - Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
5 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran

Received: 2022-08-16 Accepted : 2022-11-23 Published : 2023-09-01

Keywords: Probiotic, L.casei, Esophageal cancer, Viability assay,

Abstract :

One of the top causes of cancer-related deaths globally is esophageal cancer. In investigations of cell toxicity, the MTT test is one of the most often used cell viability/cytotoxicity assays for cellular metabolic activity. Nowadays, lactobacilli with probiotic effectiveness are now acknowledged as a prophylactic agent against cancer. The anti-tumor product of these bacteria have been designated in numerous studies. This investigation examined the probiotic Lactobacillus casei's in vivo impact on esophageal cancer. The MTT technique was used in this work to evaluate the cytotoxicity of L. casei (supernatant and full cell culture) to 5fu on the cancer cell line Kyse30. L. casei was able to decrease cell survival in supernatant and full cell culture (Kyse30). The possible impact of L. casei, particularly their supernatant, on esophageal cancer was initially evaluated in this research. As a result, lactobacilli species show promise for future research and development as cancer treatments.

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