The effects of Xylose monosaccharide on Water Buffalo (Bubalus bubalis) epididymal sperm kinetic parameters at 37 ˚ C
Subject Areas : Journal of Basic and Clinical Veterinary Medicine
1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
2 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
Keywords: sperm, Buffalo, CASA, Xylose, TCM 199,
Abstract :
The objective of this study was to test xylose monosaccharide sugar on buffalo epididymal sperm kinetic parameters at 37 ˚ C, of up to 24 h. Testes with epididymis were collected after death at urmia industrial abattoir, testes were recovered within 30 min after slaughter. Paired cauda epididymides from each mature buffalo bull were sliced and sperms transferred into tubes containing tissue culture medium (TCM, 199) with 10% bovine serum albumin at 37 ˚ C. Different levels of xylose (0,1, 3, 5, 10, 15 mM) were added into TCM-199 containing sperms (40-50×106 sperm/ml), with 10%, bovine serum albumin. Then samples were incubated at 37 ˚ C. Thereafter, at 1,3,5,7,10,12 and 24 hours incubation times, kinetics of at least 1 thousand sperms per samples were recorded with computer assisted sperm analysis (CASA). Results revealed that the motility parameters till 12 hrs did not show any significant differences between groups, actually no significant difference was observed between control and xylose groups means .But at 24 h, after incubation the means of xylose levels were lower than that of the control specially at 15 mM of xylose level the difference with control was significant (p<0.05). It is concluded that the xylose at high levels significantly reduce the kinetic parameters of buffalo bull epididymal sperm, especially at 24 h after incubation. Therefore, the usage of xylose sugar and at the high levels is not recommended for the energy supply of Buffalo bull epididymal sperm at 37 ˚ C incubation in TCM 199.