ارزیابی رشد و پاسخ¬های فیزیولوژیکی و بیوشیمیایی گونه انحصاری توسکا‌ییلاقی (Alnus subcordata C. A. Mey.) به تنش خشکی
محورهای موضوعی : تنش
مکرم روانبخش
1
(گروه پژوهشی محیط¬زیست طبیعی، پژوهشکده محیط زیست جهاد دانشگاهی، رشت، ایران؛ گروه زیست¬شناسی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران، )
بابک باباخانی
2
(گروه زیست‌شناسی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران.)
محمود قاسم نژاد
3
(گروه باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران، )
کلید واژه: سطح ویژه برگ, فعالیت آنتی¬اکسیدانی, کم آبی, گونه چوبی, هیرکانی,
چکیده مقاله :
در این تحقیق اثر تنش خشکی بر پارامترهای ریخت¬شناسی، فیزیولوژیکی و بیوشیمیایی گونه توسکا‌ییلاقی(Alnus subcordata C. A. Mey.) از گونه‌های چوبی انحصاری هیرکانی ارزیابی شده است. آزمایش در قالب طرح کاملاٌ تصادفی بر روی نهال¬های یکساله و چهار سطح تنش خشکی (25% (تنش شدید)، 50% (تنش متوسط)، 75% (تنش خفیف) و 100% (بدون تنش) ظرفیت زراعی) در شرایط گلخانه انجام شد. نتایج نشان داد که خشکی میزان رشد و زی‌توده نهال¬ها را کاهش داد. میزان افت در تیمار تنش شدید نسبت به شاهد در مشخصه¬های ارتفاع، قطر یقه، زی‌توده¬کل، سطح¬ برگ و شاخص سطح¬ویژه برگ نهال¬ها به‌ترتیب 8/41، 40، 4/72 ،9/91 و 6/49 درصد بود و نسبت ریشه به اندام هوایی 3/53 درصد افزایش را نشان داد. با افزایش شدت تنش میزان کلروفیل¬کل، کلروفیلa، کلروفیل¬b، کاهش تقریبی 50 درصدی و میزان کارتنوئیدها کاهش 4/38 درصدی را نشان داد. محتوای نسبی آب نهال¬ها، افت50/33 و 9/24 درصد در تنش شدید و متوسط نسبت به شاهد داشتند. میزان درصد افزایش پرولین و مالون¬دی¬آلدئید در تیمار تنش نسبت به شاهد به ترتیب 8/169، 7/142 درصد بود. در تیمار تنش خفیف و متوسط میزان افزایش فعالیت آنزيم سوپر¬اکسید¬دیسموتاز به‌ترتیب 25و36 درصد و پراکسیداز 140 و 148 درصد مشاهده شد. داده‌های این تحقیق نشان می¬دهد که نهال‌های توسکا‌ییلاقی (گونه¬ای تا حدودی حساس به خشکی) توانسته¬اند در سطح تیمار تنش خفیف و متوسط به کمک مکانیسم‌های تحمل به خشکی مانند کاهش رشد و زی‌توده، افزایش نسبت ریشه به اندام هوایی و افزایش نسبی فعالیت¬های آنزیمی با تنش مقابله کنند.
This study investigates the effect of drought stress on the morphological, physiological and biochemical parameters of Alnus subcordata C. A. Mey., an endemic wood species to Hyrcanian forests. The experimental design was completely randomized on one-year seedlings and four levels of drought stress (25% (severe stress), 50% (moderate stress), 75% (mild stress) and 100% (no stress) of crop capacity. The results showed that drought reduced the growth and biomass of seedlings. The decrease in severe stress treatment compared to the control in seedling height, basal diameter, total biomass, leaf area and specific leaf area were 41.8, 40, 72.4, 91.9 and 49.6% respectively. The root to shoot ratio showed an increase of 53.3%. With the increase of stress, total chlorophyll, chlorophyll a, chlorophyll b decreased by 50% and carotenoid decreased by 38.4%. The relative water content of the seedlings decreased by 33.50 and 24.9% in severe and moderate stress compared to the control treatment. The percentage increase of proline and malondealdehyde in the stress treatment compared to the control treatment was 169.8% and 142.7%, respectively. The activity of superoxide dismutase increased by 25% and 36% and peroxidase by 140% and 148% respectively in the mild and moderate stress treatment. The result showed that Caucasian alder seedling (a rather drought-sensitive species) could coped with drought in mild and moderate treatment by tolerance mechanisms such as reducing growth and biomass, increasing of the root to shoot ratio, reducing photosynthetic pigments and relative increasing in enzyme activities.
Ahani, H., Jalilvand, H., Vaezi, J. and Sadati, S. E. (2018). Drought stress on Elaeagnus rhamnoides (L.) A. Nelson seedlings morphology. Journal of Plant Ecosystem Conservation, 5(2):191 – 204 (In Persian with English summary).
Akhani, H., Djamali, M., Ghorbanalizadeh, A. and Ramezani, E. (2010). Plant biodiversity of Hyrcanian relict forests, N Iran: an overview of the flora, vegetation, palaeoecology and conservation. Pakistan Journal of Botany, 42(1): 231-258.
Asgharpour E., Azadfar, D. and Saeedi, Z. (2017). Evaluation of Acer cappadocicum Gled seedlings to drought stress. Journal of Plant Research, 30(1): 1-11(In Persian with English summary).
Barshan, M., Tabari Kouchaksaraei, M., Sadati, S.E. and Shahhoseini, R. (2016). Growth and Survival of Willow Seedling (Salix alba L.) Under Water Deficit Stress. Journal of Forest and wood product, 69(2):249-257(In Persian with English summary).
Bates, L.S., Waldran R.P. and Teare, I.D. (1973). Rapid determination of free proline for water studies. Plant and Soil, 39: 205–208.
Bhusal, N., Lee, M., Han, A.R., Han, A. and .Kim, H.S. (2020). Responses to drought stress in Prunus sargentii and Larix kaempferi seedlings using morphological and physiological parameters. Forest Ecology and Management, 465:118099.
Boor, Z., Parad, G. A., Hosseini, S. M. and Ghanbary, E. (2021). Morphological, physiological, and enzymatic responses of Caucasian alder (Alnus subcordata C. A. Mey) seedlings to water deficit conditions by inoculation of Rhizophagus irregularis. Journal of Iranian Plant Ecophysiological Research, 16(61):80-93(In Persian with English summary).
Chakhchar, A., Wahbi, S., Lamaoui, M., Ferradous, A., El Mousadik, A., Ibnsouda-Koraichi, S., Filali-Maltouf, A. and El Modafar, C. (2015). Physiological and biochemical traits of drought tolerance in Argania spinosa. Journal of plant interactions, 10(1): 252-261.
Deligoz, A. and Bayar E. (2018). Drought stress responses of seedlings of two oak species (Quercus cerris and Quercus robur). Turkish Journal of Agriculture and Forestry, 42:114-123.
Díaz-López, L., Gimeno, V., Simón, I., Martínez, V., Rodríguez-Ortega, W. M. and García-Sánchez, F. (2012). Jatropha curcas seedlings show a water conservation strategy under drought conditions based on decreasing leaf growth and stomatal conductance. Agricultural water management, 105:48-56.
Du, N, Guo, W., Zhang, X. and Wang, R. (2010). Morphological and physiological responses of Vitex negundo L. var. heterophylla (Franch.) Rehd. to drought stress. Acta physiologiae plantarum, 32:839-848.
Fang, J., Wu, F., Yang, W., Zhang, J. and Cai, H. (2012). Effects of drought on the growth and resource use efficiency of two endemic species in an arid ecotone. Acta Ecologica Sinica, 32(4):195-201.
Ge, Y, He, X, Wang, J, Jiang, B, Ye, R and Lin, X. (2014). Physiological and biochemical responses of Phoebe bournei seedlings to water stress and recovery. Acta Physiologiae Plantarum, 36:1241-1250.
Geng, D.L., Lu, L.Y., Yan, M.J., Shen, X.X., Jiang, L.J., Li, H.Y., Wang, L.P., Yan, Y., Xu, J.D., Li, C.Y. and Yu, J.T. (2019). Physiological and transcriptomic analyses of roots from Malus sieversii under drought stress. Journal of Integrative Agriculture, 18(6):1280-1294.
Ghaffari, H., Tadayon, M.R., Nadeem, M., Cheema, M. and Razmjoo, J. (2019). Proline-mediated changes in antioxidant enzymatic activities and the physiology of sugar beet under drought stress. Acta physiologiae plantarum, 41: 22-35.
Giannopolitis, C.N. and Ries, S.K. (1977). Superoxide dismutases: II. Purification and quantitative relationship with water-soluble protein in seedlings. Plant physiology. 59, 315-8.
Guo, X., Guo, W., Luo, Y., Ta, N. X, Du, N. and Wang, R. (2013). Morphological and biomass characteristic acclimation of trident maple (Acer buergerianum Miq.) in response to light and water stress. Acta Physiologiae Plantarum, 35: 1149-1159.
Guo, X., Luo, Y.J., Xu, Z.W., Li, M.Y. and Guo, W.H. (2019). Response strategies of Acer davidii to varying light regimes under different water conditions. Flora, 257:151423.
Heidari, M., Attar Roshan, S. and Abdolahzade S. (2010). Determining the suitable irrigation period of Acer monspessulanum sapling in Dareh-Shahr nursery- Ilam. Journal of Renewable Natural Resources Research, 1(2):59-71.
Hosseini Nasr, S.M., Sagheb-Talebi, K. and Hojjati, S. M. (2013). Effect of drought stress induced by altitude, on four wild almond species, Iranian Journal of Forest and Poplar Research, 21(2):373- 86.
Hu, H, Chen, H, Hu, T. and Zhang, J. (2012). Adaptability comparison between the seedlings of Eucalyptus grandis and Alnus cremastogyne under the condition of continuous drought stress. Journal of Agricultural Science, 4: 75-86.
Jahanbazy Goujani H., Hosseini Nasr S. M., Sagheb-Talebi K. and Hojjati S.M. (2013). Effect of drought stress induced by altitude, on four wild almond species, Iranian Journal of Forest and Poplar Research, 21(2):373-386(In Persian with English summary).
Kunz, J, Räder, A. and Bauhus, J. (2016). Effects of drought and rewetting on growth and gas exchange of minor European broadleaved tree species. Forests, 7: 239.
Lei, Y., Yin, C. and Li, C. (2006). Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii. Physiologia Plantarum, 127:182-191.
Li, K.R., Wang, HH, Han G, Wang, Q.J. and Fan J. (2008). Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. New Forests, 35: 255-266.
Lichtenthaler, HK. (1987). Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology. 148:350–382.
Liu, B., Liang, J., Tang, G., Wang, X., Liu, F. and Zhao, D. (2019). Drought stress affects on growth, water use efficiency, gas exchange and chlorophyll fluorescence of Juglans rootstocks. Scientia Horticulturae, 250:230-235.
Mozaffarian, V. (2004). Trees and shrubs of Iran. Farhang- e Moaser Publications, Tehran, 1003 pp.
Norouzi haroni N. and Tabari koochksaraee M. (2015). Morpho-Physiological Responses of Black Locust (Robinia pseudoacacia L.) Seedlings to Drought Stress, Journal of forest and wood products, 68(3);715-727(In Persian with English summary).
Plewa, M.J., Smith, S.R. and Wagner, E.D. (1991). Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation research/fundamental and molecular mechanisms of mutagenesis, 247(1):57-64.
Rahimi, D., Kartoolinejad, D., Nourmohammadi, K. and Naghdi, R. (2017). The effect of carbon nanotubes on drought tolerance of Caucasian alder (Alnus subcordata CA Mey) seeds in germination stage. Iranian Journal of Seed Science and Technology, 6(2):17-28.
Saxton, K.E., Rawls, W.J., Romberger, J.S. and Papendick, R.I. (1986). Estimating generalized soil-water characteristics from texture, Soil Science Society of America Journal, 50:1031-1036.
Silva, E.C.D., Silva, M.F., Nogueira, R.J. and Albuquerque, M.B. (2010). Growth evaluation and water relations of Erythrina velutina seedlings in response to drought stress. Brazilian Journal of Plant Physiology, 22:225-233.
Tariq, A., Pan, K., Olatunji, O.A., Graciano, C., Li, Z., Sun, F., Zhang, L., Wu, X., Chen, W., Song, D. and Huang, D. (2018). Phosphorous fertilization alleviates drought effects on Alnus cremastogyne by regulating its antioxidant and osmotic potential. Scientific Reports, 8:1-11.
Toscano, S., Farieri, E., Ferrante, A. and Romano, D. (2016). Physiological and Biochemical Responses in Two Ornamental Shrubs to Drought Stress. Frontiers in Plant Science,7:645.
Wang, R., Gao, M., Ji, S., Wang, S., Meng, Y. and Zhou, Z. (2016). Carbon allocation, osmotic adjustment, antioxidant capacity and growth in cotton under long-term soil drought during flowering and boll-forming period. Plant Physiology and Biochemistry, 107:137-146.
Wu, F., Bao, W., Li F. and Wu, N. (2008). Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. Environmental and experimental botany, 63:248-255.
Wu, M., Zhang, W.H., Ma, C. and Zhou, J.Y. (2013). Changes in morphological, physiological and biochemical responses to different levels of drought stress in Chinese cork oak (Quercus variabilis Bl.) seedlings. Russian journal of plant physiology, 60:681-692.
Yang, F. and Miao, L. F. (2010). Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica, 44(1):23-37.
Zarafshar, M., Akbarinia, M., Hosseini, S.M. and Rahaie, M. (2016). Drought resistance of wild pear (Pyrus Boisseriana Buhse.). Journal of forest and wood products, 69(1):97-110 (In Persian with English summary).