برهم¬کنش تنش شوری و نانوذره کیتوزان بر روی برخی از خصوصیات بیوشیمیایی و عناصر ریزمغذی برگ و ریشه ذرت (.Zea mays L)
محورهای موضوعی : تنش
سیده نسرین وقار موسوی
1
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سارا سعادتمند
2
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رشید جامعی
3
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رضا درویش زاده
4
1 - دانشکده علوم و فناوری‌های هم‌گرا، واحد علوم تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی ،تهران، ایران.
3 - گروه زیست شناسی، دانشگاه ارومیه، ارومیه، ایران
4 - گروه تولید و ژنتیک گیاهی دانشکده کشاورزی و منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران
کلید واژه: تنش محیطی, ذرت, فعالیت پاداکسندگی, فنل و فلاونوئید, کیتوزان,
چکیده مقاله :
نظر به اینکه عملکرد ذرت تحت تأثیر شوری کاهش مییابد، در تحقیق حاضر اثر این تنش بر روی رقم سینگل کراس 201 ذرت بررسی و نقش تعدیلکنندگی نانوذره کیتوزان ارزیابی شد. بدین منظور، بذور در گلدانهایی کشت شدند و در مرحله چهار برگی تیمارهای مختلف یعنی تنش شوری در سه سطح 0، 05/0 و 1/0 مولار و اسپری نانو ذره کیتوزان nm 50 با غلظتهای 0، 05/0 و 1/0 گرم بر لیتر اعمال گردید. نتایج نشان داد که تیمارهای مورد مطالعه اثر معنیداری بر صفات ذرت داشتند. صفاتی مانند فنل، فلاونوئید، آنتوسیانین، جاروبکنندگی رادیکال DPPH و محتوای نیتروژن و روی در برگ ذرت به شکل معنیداری بیشتر از ریشه آن بود. در اکثر صفات اختلاف آماری معنیداری بین تیمارهای مورد مطالعه در بافت ریشه مشاهده نشد، در حالی که در بافت برگ تفاوت معنیدار بین میانگینها وجود داشت. میزان فنل و فلاونوئید، درصد جاروبکنندگی رادیکالهای آزاد DPPH و آنتوسیانین برگ ذرت با افزایش غلظت محلولپاشی نانوذره کیتوزان افزایش و با تشدید سطح شوری کاهش معنیداری نسبت به شاهد داشتند. همچنین با افزایش تنش شوری در هر دو بافت ریشه و برگ بهوضوح میزان کلر افزایش و محتوای آهن کاهش یافت که استفاده از محلولپاشی نانوذره کیتوزان به طور معنیداری این روند را بهبود داد. با توجه به عملکرد مطلوب نانوذره کیتوزان و خطرات پایین زیست‌محیطی آن توصیه میگردد در مطالعات آتی به این موضوع توجه بیشتری شود و ارزیابیها در مراحل انتهایی رشد ذرت نیز انجام گردد.
Considering that the maize yield decreases under salinity, the effect of this stress on single cross 201 maize cultivar was investigated in the present study, and the role of chitosan nanoparticles as a moderator was evaluated. For this purpose, the seeds were grown in pots, and at the four-leaf stage, different treatments were applied, i.e. salinity stress at three levels of 0, 0.05, and 0.1 M and 50 nm chitosan nanoparticle spray with concentrations of 0, 0.05, and 0.1 g/liter. The results showed that the studied treatments had a significant effect on maize traits. Characteristics such as phenol, flavonoid, anthocyanin, DPPH radical scavenging, nitrogen, and zinc content were significantly higher in maize leaves than in their roots. In most traits, no statistically significant difference was observed between the studied treatments in the root, while in the leaf we saw a significant difference between the averages. The amount of phenol, flavonoid, DPPH radical scavenging, and anthocyanin in maize leaf increased with chitosan nanoparticle spraying and with increasing salinity level, they had a significant decrease compared to the control. Also, with the increase of salinity in both root and leaf tissues, the amount of chlorine increased, the content of iron decreased, and the application of chitosan nanoparticles significantly improved this process. Considering the optimal performance of chitosan nanoparticles and their low environmental risks, paying more attention to this issue in future studies and conducting evaluations in the final stages of maize growth is recommended.
Al-Tawaha, A.R., Turk, M.A., Al-Tawaha, A.R.M., Alu'Datt, M.H., Wedyan, M., Al-Ramamneh, E.A.D.M. and Hoang, A.T. (2018). Using chitosan to improve growth of maize cultivars under salinity conditions. Bulgarian Journal of Agricultural Science. 24(3): 437-442.
Al-Tawaha, A.R.M. and Al-Ghzawi, A. L. A. (2013). Effect of chitosan coating on seed germination and salt tolerance of lentil (Lens culinaris L.). Research on Crops. 14(2): 489-491.
Arya, S.P., Mahajan, M. and Jain, P. (2000). Non-spectrophotometric methods for the determination of Vitamin C. Analytica Chimica Acta. 417(1): 1-14.
Arzhang, S., Darvishzadeh, R., Alipour, H., Maleki, H.H. and Dezhsetan, S. (2024). Genetic variability of maize (Zea mays) germplasm from Iran: genotyping with a maize 600K SNP array and genome-wide scanning for selection signatures. Crop and Pasture Science. 75(3).
Attaran Dowom, S., Karimian, Z., Mostafaei Dehnavi, M. and Samiei, L. (2022). Chitosan nanoparticles improve physiological and biochemical responses of Salvia abrotanoides (Kar.) under drought stress. BMC Plant Biology. 22(1): 364.
Attia, M.S., Osman, M.S., Mohamed, A.S., Mahgoub, H.A., Garada, M.O., Abdelmouty, E.S. and Abdel Latef, A.A.H. (2021). Impact of foliar application of chitosan dissolved in different organic acids on isozymes, protein patterns and physio-biochemical characteristics of tomato grown under salinity stress. Plants. 10(2): 388.
Badshah, I., Mustafa, N., Khan, R., Mashwani, Z. U. R., Raja, N.I., Almutairi, M. H., Aleya. L., Sayed, A.A., Zaman, S., Sawati, L. and Sohail. (2023). Biogenic titanium dioxide nanoparticles ameliorate the effect of salinity stress in wheat crop. Agronomy. (13):352.
Bandara, S., Du, H., Carson, L., Bradford, D. and Kommalapati, R. (2020). Agricultural and biomedical applications of chitosan-based nanomaterials. Nanomaterials. 10(10): 1903.
Behboudi, F., Tahmasebi Sarvestani, Z., Kassaee, M.Z., Modares Sanavi, S.A.M., Sorooshzadeh, A. and Ahmadi, S.B. (2018). Evaluation of chitosan nanoparticles effects on yield and yield components of barley (Hordeum vulgare L.) under late season drought stress. Journal of Water and Environmental Nanotechnology. 3(1): 22-39.
Behboudi, F., Tahmasebi Sarvestani, Z., Zaman Kassaee, M., Modares Sanavi1, S.A.M. and Sorooshzadeh, A. (2019a). The effect of foliar and soil application of chitosan nanoparticles on chlorophyll, photosynthesis, yield and yield components of wheat (Triticum aestivum L.) under drought stress after pollination. Plant Process and Function. 8(30): 271-285. [In Persian].
Behboudi, F., Tahmasebi-Sarvestani, Z., Kassaee, M.Z., Modarres-Sanavy, S.A.M., Sorooshzadeh, A. and Mokhtassi-Bidgoli, A. (2019b). Evaluation of chitosan nanoparticles effects with two application methods on wheat under drought stress. Journal of Plant Nutrition. 42(13): 1439-1451.
Bittelli, M., Flury, M., Campbell, G.S. and Nichols, E.J. (2001). Reduction of transpiration through foliar application of chitosan. Agricultural and Forest Meteorology. 107(3): 167-175.
Brand-Williams, W., Cuvelier, M.E. and Berset, C.L.W.T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology. 28(1): 25-30.
Chen, M., Yang, Z., Liu, J., Zhu, T., Wei, X., Fan, H. and Wang, B. (2018). Adaptation mechanism of salt excluders under saline conditions and its applications. International Journal of Molecular Sciences. 19(11): 3668.
Cho, M.H., No, H.K. and Prinyawiwatkul, W. (2008). Chitosan treatments affect growth and selected quality of sunflower sprouts. Journal of Food Science. 73(1): S70-S77.
ChunYan, L., GuoRui, M. and WenYing, H. (2003). Induction effect of chitosan on suppression of tomato early blight and its physiological mechanism. Journal of Zhejiang University (Agriculture and Life Sciences). 29: 280-286.
Dzung, N. A. (2007). Chitosan and their derivatives as prospective biosubstances for developing sustainable eco-agriculture. Advances in Chitin Science X. 453-459.
Farooq, M., Hussain, M., Wakeel, A., & Siddique, K. H. (2015). Salt stress in maize: effects, resistance mechanisms, and management. A review. Agronomy for Sustainable Development, 35, 461-481.
Guan, Y.J., Hu, J., Wang, X.J., and Shao, C.X. (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B. 10: 427-433.
Hassan, F.A.S., Ali, E., Gaber, A., Fetouh, M.I. and Mazrou, R. (2021). Chitosan nanoparticles effectively combat salinity stress by enhancing antioxidant activity and alkaloid biosynthesis in Catharanthus roseus (L.) G. Don. Plant Physiology and Biochemistry. 162: 291-300.
Hassanvand, A., Saadatmand, S., Lari Yazdi, H. and Iranbakhsh, A. (2023). Evaluation of photosynthetic pigments, fluorescence indexes, gas exchange, and some active flavonoid substances Pansy (Viola tricolor L.) under the effect of Bio-Silver Nanoparticles. Journal of Plant Environmental Physiology. 69(1): 80-98. [In Persian].
Hidangmayum, A. and Dwivedi, P. (2022). Chitosan based nanoformulation for sustainable agriculture with special reference to abiotic stress: a review. Journal of Polymers and the Environment. 30(4): 1264-1283.
Iftikhar, N., Perveen, S., Ali, B., Saleem, M.H. and Al-Sadoon, M.K. (2024). Physiological and Biochemical Responses of Maize (Zea mays L.) Cultivars Under Salinity Stress. Turkish Journal of Agriculture and Forestry. 48(3): 332-343.
Iqbal, S., Hussain, S., Qayyaum, M.A., Ashraf, M. and Saifullah, S. (2020). The response of maize physiology under salinity stress and its coping strategies. Plant Stress Physiology. 1-25.
Kalra, N., Jain, M.C., Joshi, H.C., Choudhary, R., Harit, R.C., Vatsa, B.K., Sharma, S.K. and Kumar, V. (1998). Flyash as a soil conditioner and fertilizer. Bioresource Technology. 64(3): 163-167.
Li, C., Du, H., Wang, L., Shu, Q., Zheng, Y., Xu, Y., Zhang, J., Zhang, J., Yang, R. and Ge, Y. (2009). Flavonoid composition and antioxidant activity of tree peony (Paeonia section Moutan) yellow flowers. Journal of Agricultural and Food Chemistry. 57(18): 8496-8503.
Ma, L., Li, Y., Yu, C., Wang, Y., Li, X., Li, N., Chen, Q. & Bu, N. (2012). Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress. Protoplasma. 249: 393-399.
Malerba, M. and Cerana, R. (2016). Chitosan effects on plant systems. International Journal of Molecular Sciences. 17(7): 996.
Ministry of Jihad Agriculture. (2023). Agricultural statistics. Vol. 1: Crops. Statistics and information technology office, deputy of planning and economy. Ministry of Jihad Agriculture. [In Persian].
Munns, R. and Tester, M. (2008). Mechanisms of salinity tolerance. Annual Review of Plant Biology. 59(1): 651-681.
Nadernejad, N., Ahmadimoghadam, A., Hossyinifard, J. and Poorseyedi, S. (2013). Study of the rootstock and cultivar effect in PAL activity, production of phenolic and flavonoid compounds on flower, leaf and fruit in Pistachio (Pistacia vera L.). Journal of Plant Biological Sciences. 5(15): 95-110.
Oliveira, H.C., Gomes, B.C., Pelegrino, M.T. and Seabra, A.B. (2016). Nitric oxide-releasing chitosan nanoparticles alleviate the effects of salt stress in maize plants. Nitric Oxide. 61: 10-19.
Quitadamo, F., De Simone, V., Beleggia, R. and Trono, D. (2021). Chitosan-induced activation of the antioxidant defense system counteracts the adverse effects of salinity in durum wheat. Plants. 10(7): 1365.
Rahimian, M.H. and Gholami, H. (2022). An analysis of the salinity status of water resources in use in the agricultural sector. Journal of Water and Sustainable Development. 9(3): 107-116. [In Persian].
Razavizadeh, R., Adabavazeh, F. and Komatsu, S. (2020). Chitosan effects on the elevation of essential oils and antioxidant activity of Carum copticum L. seedlings and callus cultures under in vitro salt stress. Journal of Plant Biochemistry and Biotechnology. 29: 473-483.
Román-Doval, R., Torres-Arellanes, S.P., Tenorio-Barajas, A.Y., Gómez-Sánchez, A. and Valencia-Lazcano, A.A. (2023). Chitosan: Properties and its application in agriculture in context of molecular weight. Polymers. 15(13): 2867.
Sabagh, A.E., Çiğ, F., Seydoşoğlu, S., Battaglia, M.L., Javed, T., Iqbal, M.A. and Awad, M. (2021). Salinity stress in maize: Effects of stress and recent developments of tolerance for improvement. Cereal Grains. 1: 213.
Sen, S.K., Chouhan, D., Das, D., Ghosh, R. and Mandal, P. (2020). Improvisation of salinity stress response in mung bean through solid matrix priming with normal and nano-sized chitosan. International Journal of Biological Macromolecules. 145: 108-123.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16(3): 144-158.
Soni, A.T., James, E., Rookes, E. and Arya.S. (2023). Chitosan Nanoparticles as Seed Priming Agents to Alleviate Salinity Stress in Rice (Oryza sativa L.) Seedlings. Polysaccharides (4): 129–141.
Thambiliyagodage, C., Jayanetti, M., Mendis, A., Ekanayake, G., Liyanaarachchi, H. and Vigneswaran, S. (2023). Recent advances in chitosan-based applications—a review. Materials. 16(5): 2073.
Yousefi, K., Jamei, R. and Darvishzadeh, R. (2022). Study of some physiological and biochemical parameters of Okra plant (Abelmoschus esculentus L.) under salt stress in the presence of chitosan nanoparticles. Iranian Journal of Plant Biology. 14(1): 2322-2204. [In Persian].
Zayed, M.M., Elkafafi, S.H., Zedan, A.M. and Dawoud, S.F. (2017). Effect of nano chitosan on growth, physiological and biochemical parameters of Phaseolus vulgaris under salt stress. Journal of Plant Production. 8(5): 577-585.
Zhang, G., Wang, Y., Wu, K., Zhang, Q., Feng, Y., Miao, Y. and Yan, Z. (2021). Exogenous application of chitosan alleviate salinity stress in lettuce (Lactuca sativa L.). Horticulturae. 7(10): 342.