The Combined Effects of Eccentric Resistance Traning and Blood Flow Restriction on the Level of Interleukin 15 and Tumor necrosis factor-alpha on Non-Athletes
Subject Areas : Exercise Physiology and Performance
Mohammad Keshavarz
1
,
Farzaneh Taghian
2
,
Fatemeh Zahra Abdollahi
3
,
Sara Norouzi
4
1 - Secretary of Physical Education, Department of Education, Shahin Shahr District, Shahin Shahr, Iran.
2 - Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
3 - Department of Sports Physiology, Faculty of Sports Sciences, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
4 - Department of Plant Biotechnology, Medicinal Plants Research Centre, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Keywords: Eccentric resistance exercise, Interleukin 15, Training with Blood Flow restriction, Tumor necrosis factor.,
Abstract :
Background: The present study aimed to determine the effect of a session eccentric resistance exercise with and without blood flow restriction on Interleukin 15 and Tumor necrosis factor-alpha in nonathlete People. Methods: In a crossover research design, 36 male non-athletes (with an average mean of 25± 2.9 age, 22± 1.39 kg/m2 mass body index) were asked to participate in the study randomly. The exercise consisted of one lower limb resistance session and one set until exhaustion. The exercise consisted of one lower limb resistance session and one set until exhaustion. The exercise intensity in each group was 30% of One Repetition Maximum Respectively. The blood flow Restriction was done by the proximal pressure gauge on the thigh. The blood samples were taken 48 hours before the test. The blood samples were analyzed to determine Interleukin 15 and Tumor necrosis factor-alpha. The statistical analysis was examined based on the study's objectives through descriptive statistics, correlative, and independent tests(P=0.05). Results: The result showed there is a meaningful increase in Interleukin 15 after one session of eccentric resistance exercise accompanied by blood flow(P=0.000), but in comparison to the control group, the Tumor necrosis factor-alpha in the experiment group showed a meaningful decrease (P=0.000). Conclusion: It seems low-intensity eccentric resistance exercise for one session accompanied by blood flow Restriction could have an appropriate effect on the discharge rate of muscle growth factor and a decrease in inflammatory factors derived from physical activity.
1. Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to resistance exercise and training. Sports medicine. 2005;35:339-61.
2. Wernbom M, Paulsen G, Nilsen TS, Hisdal J, Raastad T. Contractile function and sarcolemmal permeability after acute low-load resistance exercise with blood flow restriction. European journal of applied physiology. 2012;112:2051-63.
3. Fujita S, Abe T, Drummond MJ, Cadenas JG, Dreyer HC, Sato Y, et al. Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis. Journal of applied physiology. 2007;103(3):903-10.
4. Anderson JE. A role for nitric oxide in muscle repair: nitric oxide–mediated activation of muscle satellite cells. Molecular biology of the cell. 2000;11(5):1859-74.
5. Reeves GV, Kraemer RR, Hollander DB, Clavier J, Thomas C, Francois M, et al. Comparison of hormone responses following light resistance exercise with partial vascular occlusion and moderately difficult resistance exercise without occlusion. Journal of applied physiology. 2006;101(6):1616-22.
6. Takarada Y, Nakamura Y, Aruga S, Onda T, Miyazaki S, Ishii N. Rapid increase in plasma growth hormone after low-intensity resistance exercise with vascular occlusion. Journal of applied physiology. 2000;88(1):61-5.
7. Loenneke JP, Fahs C, Rossow L, Abe T, Bemben M. The anabolic benefits of venous blood flow restriction training may be induced by muscle cell swelling. Medical hypotheses. 2012;78(1):151-4.
8. Bodine SC, Stitt TN, Gonzalez M, Kline WO, Stover GL, Bauerlein R, et al. Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo. Nature cell biology. 2001;3(11):1014-9.
9. Frigeri A, Nicchia GP, Balena R, Nico B, Svelto M. Aquaporins in skeletal muscle: reassessment of the functional role of aquaporin‐4. The FASEB Journal. 2004;18(7):905-7.
10. Patterson SD, Leggate M, Nimmo MA, Ferguson RA. Circulating hormone and cytokine response to low-load resistance training with blood flow restriction in older men. European journal of applied physiology. 2013;113:713-9.
11. Quinn LS. Interleukin-15: a muscle-derived cytokine regulating fat-to-lean body composition. Journal of animal science. 2008;86(suppl_14):E75-E83.
12. Ciciliot S, Schiaffino S. Regeneration of mammalian skeletal muscle: basic mechanisms and clinical implications. Current pharmaceutical design. 2010;16(8):906-14.
13. Andersson H, Bøhn S, Raastad T, Paulsen G, Blomhoff R, Kadi F. Differences in the inflammatory plasma cytokine response following two elite female soccer games separated by a 72‐h recovery. Scandinavian journal of medicine & science in sports. 2010;20(5):740-7.
14. Pedersen BK. Muscles and their myokines. Journal of Experimental Biology. 2011;214(2):337-46.
15. Quinn LS, Anderson BG, Drivdahl RH, Alvarez B, Argilés JM. Overexpression of interleukin-15 induces skeletal muscle hypertrophy in vitro: implications for treatment of muscle wasting disorders. Experimental cell research. 2002;280(1):55-63.
16. Carbo N, Lopez-Soriano J, Costelli P, Busquets S, Alvarez B, Baccino FM, et al. Interleukin-15 antagonizes muscle protein waste in tumour-bearing rats. British journal of cancer. 2000;83(4):526-31.
17. Pistilli EE, Siu PM, Alway SE. Interleukin-15 responses to aging and unloading-induced skeletal muscle atrophy. American Journal of Physiology-Cell Physiology. 2007;292(4):C1298-C304.
18. Pedersen BK, Febbraio MA. Muscle as an endocrine organ: focus on muscle-derived interleukin-6. Physiological reviews. 2008;88(4):1379-406.
19. Paulsen G, Ramer Mikkelsen U, Raastad T, Peake JM. Leucocytes, cytokines and satellite cells: what role do they play in muscle damage and regeneration following eccentric exercise? Exercise immunology review. 2012;18.
20. Thiebaud RS, Yasuda T, Loenneke JP, Abe T. Effects of low-intensity concentric and eccentric exercise combined with blood flow restriction on indices of exercise-induced muscle damage. Interventional Medicine and Applied Science. 2013;5(2):53-9.
21. Brown SJ, Child RB, Day SH, Donnelly AE. Indices of skeletal muscle damage and connective tissue breakdown following eccentric muscle contractions. European journal of applied physiology and occupational physiology. 1997;75:369-74.
22. Pedersen BK, Åkerström TC, Nielsen AR, Fischer CP. Role of myokines in exercise and metabolism. Journal of applied physiology. 2007.
23. Khan SS, Smith MS, Reda D, Suffredini AF, McCoy Jr JP. Multiplex bead array assays for detection of soluble cytokines: comparisons of sensitivity and quantitative values among kits from multiple manufacturers. Cytometry Part B: Clinical Cytometry: The Journal of the International Society for Analytical Cytology. 2004;61(1):35-9.
24. Nieman DC, Dumke CL, Henson DA, McAnulty SR, Gross SJ, Lind RH. Muscle damage is linked to cytokine changes following a 160-km race. Brain, behavior, and immunity. 2005;19(5):398-403.
25. Gleeson M. Immune function in sport and exercise. Journal of applied physiology. 2007.
26. Nielsen AR, Mounier R, Plomgaard P, Mortensen OH, Penkowa M, Speerschneider T, et al. Expression of interleukin‐15 in human skeletal muscle–effect of exercise and muscle fibre type composition. The Journal of physiology. 2007;584(1):305-12.
27. Riechman SE, Balasekaran G, Roth SM, Ferrell RE. Association of interleukin-15 protein and interleukin-15 receptor genetic variation with resistance exercise training responses. Journal of Applied Physiology. 2004;97(6):2214-9.
28. Louis E, Raue U, Yang Y, Jemiolo B, Trappe S. Time course of proteolytic, cytokine, and myostatin gene expression after acute exercise in human skeletal muscle. Journal of applied physiology. 2007;103(5):1744-51.
29. Nielsen JL, Aagaard P, Bech RD, Nygaard T, Hvid LG, Wernbom M, et al. Proliferation of myogenic stem cells in human skeletal muscle in response to low‐load resistance training with blood flow restriction. The Journal of physiology. 2012;590(17):4351-61.
30. Nielsen AR, Hojman P, Erikstrup C, Fischer CP, Plomgaard P, Mounier R, et al. Association between interleukin-15 and obesity: interleukin-15 as a potential regulator of fat mass. The Journal of Clinical Endocrinology & Metabolism. 2008;93(11):4486-93.