تاثير مصرف جيرههای مكملشده با سطوح مختلف گوانيدينواستيكاسيد بر صفات لاشه، برخی فراسنجههای بيوشيميايی خون، فعاليت آنزیمهای كبدی، جمعيت ميكروبی و ريختشناسی روده در بلدرچینهای گوشتی ژاپنی
محورهای موضوعی : فصلنامه زیست شناسی جانوریرضا علي پور فيل آبادي 1 , مصطفی فغانی 2 * , فرشید خیری 3
1 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - گروه علوم دامی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
کلید واژه: گوانيدينو استيك اسيد, فراسنجههاي بيوشيمايي خون, آنزیم كبدي, ريخت شناسي روده, بلدرچين ژاپني,
چکیده مقاله :
به منظور بررسي اثر مصرف جيرههاي مكمل شده با سطوح مختلف گوانيدينواستيكاسید بر عملكرد، برخي فراسنجههاي بيوشيميايي خون، جمعيت ميكروبي و ريختشناسي روده در بلدرچینهاي گوشتي ژاپني، تعداد 288 قطعه بلدرچین ژاپنی در سن 1-35 روزگی با 4 تیمار آزمایشی شامل گروه شاهد و مصرف کننده گوانيدينواستيكاسيد با سطوح 5/0 و 1 و 5/1 گرم/کیلوگرم از خوراک استاندارد با 6 تکرار و 12 قطعه بلدرچین تخمگذار در هر تکرار استقاده گرديد. در انتهاي دوره (35 روزگي) پس از كشتار دو قطعه پرنده از هر تكرار، صفات لاشه بلدرچینها، فراسنجههاي خوني نظير كلسترول، تريگليسيريد، لیپوپروتئین با چگالی بسیار کم (VLDL) و لیپوپروتئین با چگالی بالا (HDL) و همچنين فعاليت آنزیمهاي كبدي نظير آلكالين فسفاتاز (ALP)، آلانينترانسآميناز (ALT) و آسپارتاتترانسآميناز (AST) بررسي شد. برای ارزيابي بافت و جمعيت فلور ميكروبي روده باريك، نمونهبرداري صورت گرفت. نتايج نشان داد مصرف جيرههاي مكمل شده با گوانيدينواستيك اسيد سبب بهبود معنيدار صفات لاشه در بلدرچینهاي تحت مطالعه شد (05/0 ≤ p). مكملسازي جيره با گوانيدينواستيك اسيد سبب كاهش معنيدار بر غلظت سرمي فراسنجههاي خوني كلسترول، تري گليسريد و LDL و متعاقبا افزايشHDL شد (05/0 ≤ p). بهبود وضعيت فعاليت آنزیمهاي كبدي تحت تاثير مصرف سطوح گوانيدينواستيكاسيد مشاهده گرديد (05/0 ≤ p). افزايش معنيدار جمعيت ميكروبي مفيد لاكتوباسيلوسها و در مقابل كاهش جمعيت كلوني باكتريهاي بيماريزاي اشرشیاكلي و سالمونلا انتريكا در روده همراه با افزايش سطح ويليها و تراكم تعداد سلولهاي گابلت نشاندهنده اثر مكملسازي سطوح مختلف گوانيدينو استيك اسيد بر بهبود وضعيت سلامت روده در بلدرچینهاي تحت مطالعه بود (05/0 ≤ p). نتايج اين مطالعه ننشانگر سودمندي مصرف سطوح مختلف گوانيدينو استيكاسيد بر صفات لاشه، فراسنجههاي بيوشيميايي خون، آنزیمهاي كبدي، ريختشناسي و جمعيت ميكروبي روده در بلدرچینهاي ژاپني بود.
In order to investigate the effect of consuming diets supplemented with different levels of guanidinoacetic acid on performance, some biochemical parameters of blood, microbial population and intestinal morphology in Japanese meat quails, 288 Japanese quails aged 1-35 days were used with 4 experimental treatments including control group and consuming guanidinoacetic acid with levels of 0.5, 1 and 1.5 g/kg of standard feed with 6 replications and 12 laying quails in each replication. At the end of the period (35 days of age), after slaughtering two birds from each replicate, carcass traits of quails, blood parameters such as cholesterol, triglyceride, very low density lipoprotein (VLDL) and high density lipoprotein (HDL) as well as the activity of liver enzymes such as alkaline phosphatase (ALP), alanine transaminase (ALT) and aspartate transaminase (AST) were examined. Sampling was performed to evaluate the tissue and population of the small intestine microbial flora. The results showed that the consumption of diets supplemented with guanidinoacetic acid significantly improved carcass traits in the quails under study (p ≤ 0.05). Supplementation of the diet with guanidinoacetic acid caused a significant decrease in serum concentrations of blood parameters of cholesterol, triglycerides and LDL and subsequently increased HDL (p ≤ 0.05). Improvement in liver enzyme activity was observed under the influence of guanidinoacetic acid levels (p ≤ 0.05). A significant increase in the beneficial microbial population of Lactobacilli and, on the contrary, a decrease in the colony population of pathogenic bacteria Escherichia coli and Salmonella enterica in the intestine, along with an increase in the level of villi and the density of the number of goblet cells, indicated the effect of supplementation with different levels of guanidinoacetic acid on improving intestinal health in the quails under study (p ≤ 0.05). The results of this study showed the benefits of consuming different levels of guanidinoacetic acid on carcass traits, blood biochemical parameters, liver enzymes, morphology, and gut microbial population in Japanese quails.
.
1. Ahmadipour, B., Khajali, F., Sharifi, M.R. 2018. Effect of guanidinoacetic acid supplementation on growth performance and gut morphology in broiler chickens. Poultry Science Journal, 6:19-24.
2. Al-Daraji, H.J., Salih, A.M. 2012. Effect of dietary L-arginine on carcass traits of broilers. Animal Veterinary Science, 2:40-44.
3. AOAC. 2005. Official methods of analysis, Association of official analytical chemists. AOAC Press, Gaithersburg, USA.
4. Darmankohi, H., Tavakoli, P., Mohit, A., Mohammadpour, F. 2017. Effects of dietary supplementation of Guanidino acetic acid and L-arginine on performance, blood metabolites and carcass characteristics of Japanese quails. Animal Production Research, 8.9.2:1-10.
5. Davis, S.L. 2011. Plasma levels of prolactin, growth hormone, and insulin in sheep following the infusion of arginine, leucine and phenylalanine. Endocrinology, 91:549-555.
6. Dilger, R.N., Bryant-Angeloni, K., Payne, R.L., Lemme, A., Parsons, C.M. 2013. Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks. Poultry Science, 92:171-177.
7. Duncan, D.B. 1995. Multiple range test and F-test. Biometrics, 11:1-42.
8. Ebrahimi, M., Zare, A., Shivazad, M., Ansari Pirsaraei, Z., Tebyanian, M., Adib Moradi, M., nourijelyani, K.2015. The Effects of L-arginine Supplement on Growth, Meat Production, and Fat Deposition in Broiler Chickens. Iranian Journal of Animal Science Research, 5(4):281-290.
9. Fathi., M. 2015. Effects of L-Arginine levels supplementation on some blood parameters, thyroid hormones, mortality and performance of broilers with cold-induced ascites. Research Journal of Livestock Science, 107:195-206.
10. Fernandes, J.I.M., Murakami, A.E., Martins, E.N., Sakamoto, M.I., Garcia, E. R.M. 2009. Effect of arginine on the development of the pectoralis muscle and the diameter and the protein: deoxyribonucleic acid rate of its skeletal myofibers in broilers. Poultry Science, 88:1399-1406.
11. Fouad, A.M., El-Senousey, H.K., Yang, X.J., Yao, H. 2013. Dietary L-arginine supplementation reduces abdominal fat content by modulating lipid metabolism in broiler chickens. Animal, 7:1239-1245.
12. Halle, I., M. Henning and P. Kohler. 2006. Studies of the effects of creatine on performance of laying hens, on growth and carcass quality of broilers. Landbauforschung Volkenrode, 56:11-18.
13. Khalil, S., Saenbungkhor, N., Kesnava, K., Sivapirunthep, P., Sitthigripong, R., Jumanee, S., Chaosap, C. 2021. Effects of guanidinoacetic acid Supplementation on Productive performance, pectoral myopathies, and meat quality of broiler chickens. Animals, 11(11):3180.
14. Khajali, F., Wideman R.F. 2010. Dietary arginine: Metabolic, environmental, immunological andphysiological interrelationships. World’s Poultry Science Journal, 66: 751-766.
15. Lemme, A., Ringel, J., Rostagno, H.S., Redshaw, M.S. 2007. Supplemental guanidino acetic acid improved feed conversion, weight gain, and breast meat yield in male and female broilers.16th European Symposium on Poultry Nutrition, pp:26-30.
16. Lev, R., Spicer, S.S. 1964. Specific staining of sulphate groups with Alcian blue at low pH. Journal of Histochemical and Cytochemistry, 12:309-310.
17. Michiels, J., Maertens, L., Buyse, J., Lemme, A., Rademacher, M., Dierick, N.A. De Smet, S. 2012. Supplementation of guanidinoacetic acid to broiler diets: Effects on performance, carcass characteristics, meat quality, and energy metabolism. Poultry Science, 91:402-412.
18. Mousavi, S.N., Afsar, A., Lotfollahian, H. 2013. Effects of guanidinoacetic acid supplementation to broiler diets with varying energy contents. Poultry Science, 22:47-54.
19. Nasiroleslami, M., Torki, M., Saki, A.A., Abdolmohammadi A.R. 2018. Effects of dietary guanidinoacetic acid and betaine supplementation on performance, blood biochemical parameters and antioxidant status of broilers subjected to cold stress. Journal of Applide Animal Research, 46:1016-1022
20. NRC., National Research Council. 1994. Nutrient requirements of poultry.9th Rev.Ed., Washington, DC. National Academy Press.
21. Ostojic, S.M. 2015. Advanced physiological roles of guanidinoacetic acid. European Journal of Nutrition, 54:1211-1215.
22. Rahimian, Y., Kheiri, F., Faghani, M. 2024. Evaluation the effect of dietary vitamin E, sesamin and thymoquinone bioactive compounds on immunological response, intestinal traits and MUC-2 gene expression in broiler Japanese quails (Coturnix japonica). Animal Biotechnology, 35(1):1-11.
23. Raei, A., Karimi, A., Sadeghi, A. 2019. Performance, antioxidant status, nutrient retention and serum profile responses of laying Japanese quails to increasing addition levels of dietary guanidinoacetic acid. Italian Journal of Animal Science, 19(1):75-85.
24. Ringel, J., Lemme, A., Araujo, L.F. 2008. The effect of supplemental guanidinoacetic acid in Brazilian type broiler diets at summer conditions. Poultry Science, 87:154.
25. SAS Institute. 2001. SAS.STAT user’s guide for personal computer. Release 6.12 SAS Institute, Inc., Cary, N.C., USA.
26. Tossenberger, J., Rademacher, M., Nemeth, K., Halas, V., Lemme, A. 2016. Digestibility and metabolism of dietary guanidinoacetic acid fed to broilers. Poultry Science, 95:2058-2067.
27. Vali N. 2008. The Japanese quail. A Review. International Journal of Poultry Science, 7:925-931.
28. Wang, L., Shi, B., Shan, A., Zhang, Y. 2012. Effects of guanidinoacetic acid on growth performance, meat quality and antioxidation in growing-finishing pigs. Journal of Animal Veterinary Advances, 11:631-636.
29. Wu, L.Y., Fang, Y.J., Guo, X.Y. 2011. Dietary L-arginine supplementation beneficially regulates body fat deposition of meat type ducks. British Poultry Science, 52: 221-226.
30. Wyss, M., Kaddurah-Daouk, R. 2000. Creatine and creatinine metabolism. Physiological Review, 80:1107-1213.