اثر حفاظتی کورکومین بر میزان ترانس‌آمینازهای کبدی و آلکالین فسفاتاز در موش های صحرایی نر به دنبال دریافت نانوذرات نیکل
محورهای موضوعی : فصلنامه زیست شناسی جانوریرحیمه خضری مطلق 1 , اکبر وحدتی 2 , سیدابراهیم حسینی 3 , محمدامین عدالت منش 4
1 - گروه زیست شناسی، پردیس واحد علوم و تحقیقات فارس، دانشگاه آزاد اسلامی، فارس، ایران|گروه زیست شناسی،واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
2 - گروه زیست شناسی،واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
3 - گروه زیست شناسی،واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
4 - گروه زیست شناسی،واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
کلید واژه: ﮐﻮرﮐﻮﻣﯿﻦ, نانوذرات نیکل, آﺳﭙﺎرﺗﺎت آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز, آﻻﻧﯿﻦ آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز و آﻟﮑﺎﻟﯿﻦ ﻓﺴﻔﺎﺗﺎز,
چکیده مقاله :
نانوذرات نیکل به صورت گسترده در محیط زیست پراکنده است که مواجهه شدن با آن باعث آسیب به بافت های مختلفی بدن از جمله شش، کبد، بیضه و کلیه می شود، که تاثیرات عمده آن بر بافت کبد می باشد، بنابراین هدف از این مطالعه بررسی اثر حفاظتی کورکومین بر میزان آنزیم های کبدی ALT، AST و ALP به دنبال دریافت نانوذرات نیکل در موش های صحرایی نر می باشد. در این مطالعه تجربی، 50 سر موش صحرایی نر نژاد ویستار به صورت تصادفی در 5 گروه (کنترل، شاهد، گروه دریافت کننده (50 میلی گرم بر کیلوگرم نیکل)، گروه دریافت کننده (50 میلی گرم بر کیلوگرم نیکل+ 150 میلی گرم بر کیلوگرم کورکومین) و گروه دریافت کننده (50 میلی گرم بر کیلوگرم نیکل+ 300 میلی گرم بر کیلوگرم کورکومین) قرار گرفتند. تیمار موش های صحرایی به مدت 28 روز ادامه داشت. در پایان پس از خونگیری از موش ها، آنزیم های (ALT، AST و ALP) بررسی شد. نانوذره نیکل ﺑﺎﻋﺚ اﯾﺠﺎد ﺗﻔﺎوت ﻣﻌﻨﯽ دار در ﻣﯿﺰان ﺳﺮﻣﯽ آﻧﺰﯾﻢﻫﺎی آﺳﭙﺎرﺗﺎت آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز، آﻻﻧﯿﻦ آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز و آﻟﮑﺎﻟﯿﻦ ﻓﺴﻔﺎﺗﺎز در سطح 0001/0> p < /em> ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل شد. در حالی که کورکومین در دوزﻫﺎی ﻣﺨﺘﻠﻒ ﺑﺎﻋﺚ ﮐﺎﻫﺶ ﻣﻌﻨﯽدار ﺳﻄﺢ ﺳﺮﻣﯽ آﻧﺰﯾﻢﻫﺎی ﺑﯿﺎن ﺷﺪه در سطح 0001/0>p < /em> ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﮐﻨﺘﺮل گردید. تیمار با ﮐﻮرﮐﻮﻣﯿﻦ موجب تخفیف آسیب کبدی القا شده با نانوذره نیکل می شود. همچنین می توان گفت ﻣﺼﺮف ﻫﻤﺰﻣﺎن ﮐﻮرﮐﻮﻣﯿﻦ ﺑﺎ نانوذره نیکل ﺑﻪ دﻟﯿﻞ ﺧﻮاص آﻧﺘﯽاﮐﺴﯿﺪاﻧﯽ ﮐﻮرﮐﻮﻣﯿﻦ، ﺑﺎﻋﺚ ﮐﺎﻫﺶ این ﻓﻌﺎﻟﯿﺖ می شود.
Nickel nanoparticles are widely distributed in the environment, resulting in damage to various tissues of body, including the lung, liver, testis, and kidney which is the major effect on the liver tissue. Therefore, this study aims to investigate Curcumin protective effect on the number of enzymes (ALT, AST, and ALP) to obtain nickel nanoparticles in rats. In this experimental study, 50 Wistar adult male rats were randomly selected and divided in to five groups (control, witness, receiver group (nickel 50 mg/kg), receiver group (nickel 50 mg/kg + Curcumin 150 mg/kg), and receiver group (nickel 50 mg/kg + Curcumin 300 mg/kg). The treatment of rats continued for 28 days. In the end, after bloodletting of rats, (ALT, AST and ALP) enzymes were investigated. Nickel nanoparticles caused a significant difference in Serum level of Aspartate Aminotransferase, Alanine aminotransferase, and Alkaline Phosphatase enzymes in the class of p < /em> ˂ 0.0001 than the control group. While, Curcumin in different dosages caused a significant reduction in the serum level of enzymes expressed at the class of p < /em> ˂ 0.0001 ratio to the control group. The treatment by Curcumin results in reducing the liver damage induced by nickel nanoparticles. Also, we can say that the concurrent consumption of Curcumin with nickel nanoparticle due to the antioxidant properties of Curcumin reduces this activity
1. Abubakar K., Mailafiya M., Chiroma M., 2020. Ameliorative effect of curcumin on lead‐induced hematological and hepatorenal toxicity in a rat model. Journal of Biochemical and Molecular Toxicology, 36(4): 1-15.
2. Abudayyak M., Guzel, E., Ozhan, G., 2017. Nickel oxide nanoparticles induce oxidative DNA damage and apoptosis in kidney cell line (NRK-52E). Journal Biological Trace Element Research, 178(1):98-104.
3. Ahmad J., Alhadlaq H.A., Siddiqui, M.A., Saquib Q., Al-Khedhairy A.A., Musarrat J., Ahamed M., 2015. Concentration-dependent induction of reactive oxygen species, cell cycle arrest and apoptosis in human liver cells after nickel nanoparticles exposure. Journal Environtal Toxicology, 30(2):137-148.
4. Afrasiabie M, Mokhtari M., 2016. Effect of Dianthus carryophyllu extract on the induced hepatotoxicity by Gentamicin in Wistar Rats. Journal of Gorgan University of Medical Sciences, 18:22-9.
5. Aggarwal B.B., 2008. Prostate cancer and curcumin: add spice to your life. Journal Cancer Biology Therapy. 7(9): 1436-1440.
6. El-Wakf AM., Elhabiby ESM., El-kholy WM, El-Ghany EA., 2011. Use of Tumeric and Curcumin to Alleviate Adverse Reproductive Outcomes of Water Nitrate Pollution in Male Rats. Journal Nature and Science. 9(7): 229-39.
7.Fallahi S., Hooshmandi Z., Setorki S., 2017. [The effects of Fe4NiO4Zn nanoparticles on thyroid tissue and serum level of T3 and T4 and TSH]. J Shahrekord University of Medical Sciences, 18:115-2.
8. Fernando S., Wijewickrama A., Gomes L., Punchihewa C.T., Madusanka S.D.P., Dissanayake H, 2016. Patterns and causes of liver involvement in acute dengue infection. Journal part of springer nature Infectious Diseases, 16: 319-326.
9. Gressner OA., Weiskirchen R., Gressner A.M., 2007. Biomarkers of liver fibrosis: Clinical translation of molecular pathogenesis or based on liver-dependent malfunction tests. International Journal of Clinical Chemistry and Diagnostic Laboratory Medicine. 381 (2): 107-113.
10. Gu Y., Wang Y., Zhou Q., Bowman L., Mao G., Zou B., Xu J., Liu Y., Liu K., Zhao J., Ding M, .2016. Inhibition of Nickel Nanoparticles-Induced Toxicity by Epigallocatechin-3-Gallate in JB6 Cells May Be through. Journal peer-reviewed open access scientific One. 4:11(3) ;55-62.
11. Ioannou G.N., Weiss N.S., Boyko E.J., Mozaffarian D., Lee S.P., 2006. Elevated Serum Alanine Aminotransferase Activity and Calculated Risk of Coronary Heart Disease in the United States. National Center for Biotechnology Information, 43: 1145-1151.
12. Jacob S.E., Moennich J.N., McKean B.A., Zirwas M.J, Taylor J.S, 2009. Nickel allergy in the United States: a public health issue in need of a ‘‘nickel directive’’. Journal of the American Academy of Dermatology; 60(6):1067–69.
13. Jayaprakasha G.K., Jaganmohan L.J., Sakariah K.K., 2006. Antioxidant activities of curcumin, demethoxycurcumin and bisdemethoxycurcumin. Journals Food Chem, 98(4): 720-724.
14. Jian T., Reddy M., Morales M, 2008. Biodistribution clearance and biocompability of Iron oxide magnetic nanoparticle in Rat. National Center for Biotechnology Information, 5:127-316.
15. Johnston R.L, 2012. Wilcoxon J. Metal Nanoparticles and Nanoalloys. Nanobiotechnology, P.1-5.
16. Katsnelson B.A., Minigaliyeva I.A., Panov V.G., Privalova L.I., Varaksin A.N., Gurvich V.B, 2015. Some patterns of metallic nanoparticles' combined subchronic toxicity as exemplified by a combination of nickel and manganese oxide nanoparticles. Journal Food and Chemical Toxicol, 86:351-6.
17. Mailafiya M., Abubakar K., Chiroma S., Danmaigoro A., 2020. Curcumin-loaded cockle shell-derived calcium carbonate nanoparticles: A novel strategy for the treatment of lead-induced hepato-renal toxicity in rats. Journal Pre-proofs, 45(2):42-56.
18. Magaye R, Yue X., Zou B., Shi H., Yu H., Liu K., 2014. Acute toxicity of nickel nanoparticles in rats after intravenous injection. National Journal Nanomedicine, 9: 1393–1402.
19.Mamonova I.A., 2013. Study of the antibacterial action of metal nanoparticles on clinical strains of gramnegative bacteria. World Journal Medical Sciences, 8(4): 3147.
20. Marzban A., Seyedalipour B., Mianabadi M., 2017. Taravati A. Investigation of the Enzyme activities of Alkaline Phosphatase, Lactate Dehydrogenase, Transaminase and Histopathological Changes of Liver after Exposure to NiO and NiO Nanoparticles in Rats. Journal of Shahid Sadoughi, 25(5): 381-395.
21. Munoz A., Costa M., 2012. Elucidating the mechanisms of nickel compound uptake: A review of particulate and nanonickel endocytosis and toxicity. Journal Toxicology and Applied Pharmacology, 260: 1-16.
22.Nie J., Pan Y., Shi J., Guo Y., Yan Z., Duan X., 2015. A Comparative study on the uptake and toxicity of nickel added in the form of different salts to maize seedlings. International Journal of Environmental Research and Public Health,12(12): 15075-15087.
23. Oh S.Y., Cho Y.K., Kang M.S., Yoo T.W., Park J.H., Kim H.J., Park D.I., Sohn C.I., Jeon W.K., Kim B.I., 2006. The association between increased alanine aminotransferase activity and metabolic factors in nonalcoholic fatty liver disease. Journal National Center for Biotechnology Information,55: 1604-1609.
24. Ozer J., Ratner M., Shaw M., Bailey W., Schomaker S., 2008. The current state of serum biomarkers of hepatotoxicity. Journal Biomarkers of Toxicity, 245(3): 194-205.
25. Patlolla, A.K., Kumari, S.A., Tchounwou, P.B., 2019. A comparison of poly ethylene glycol coated and uncoated gold nanoparticle mediated hepatotoxicity and oxidative stress in Sprague Dawleyrats. International Journal of Nanomedicine, 14: 639–647.
26. Raju S.B.G., Battu R.G., Manju latha Y.B., Srinivas K., 2012. Antihepatotoxic activity of smilax china roots on CCL4 induced hepatic damage in rats. International Journal of Pharmacy and Pharmaceutical Sciences, 4: 494-496.
27. Rivera-Espinoza Y., Muriel P., 2009. Pharmacological actions of curcumin in liver diseases or damage. National Center for Biotechnology Information, 29(10): 1457-1466.
28. Yang C., Zhang X., Fan H., Liu Y., 2009. Curcumin upregulates transcription factor Nrf2, HO expression and protects rat brains against focal ischemia. National Center for Biotechnology Information, 1282: 133-141.
29. Zarei M., Shivanandappa T., 2013. Amelioration of cyclophosphamide-induced hepatotoxicity by the root extract of Decalepis hamiltonii in mice. Journal Food Chemistry and Toxicology, 57: 179-184
_||_