Elmira Ziya Motalebipour 134*, Akbar Pirestani 234 1Department of Agronomy and Plant Breeding, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. 2Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. 3Medicinal plants research center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. 4Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran. *Corresponding Author E-mail:*e.ziyamotalebipour@khuisf.ac.ir. Received: 20 October 2022 Accepted: 8 January 2023 Abstract Wide variety of medicinal plants showed the high amount of secondary metabolites. Flavonoids are a class of important secondary metabolites with medicinal benefits. One of the well-known traditional medicinal herb is Salvia genus with a broad diversity of flavonoids. Flavonol synthase (FLS) is one of the flavonoid biosynthesis-related gene were identified in some medicinal plant of Lamiacea family. However, flavonol synthase (FLS) have not been phylogeny and evolutionary divergence analyzed in different Saliva species. In this study, Arabidopsis thaliana FLS gene (NCBI accession NM_120951) was select and used as a query sequence for performing a BLASTN search. Six sequences query which was found by NCBI platform was selected for further analysis. Phylogenetic trees of full length sequences of different FLS gene of Salvia genus were constructed by the Maximum Likelihood method using MEGA software. This study may be the reference value for the genetic relationship among different FLS gene in Salvia genus species. This variation provides us the possibility the design the primer to achieve us to find the exact FLS gene and also showed the evolutionary divergence between salvia species. پرونده مقاله