Easy operation, eco-friendliness, and high capacity are the advantages of using hydrogelbased membranes in water treatment, medicine, drug delivery, wound care products, and protective clothing. In the present work, we tried to prepare bio-films with antimicrobial ability by incorporating of Cu ions in chitosan and poly vinyl poly Pyrrolidone. The prepared film was characterized by the thermal gravimetric analysis (TGA), Fourier transforms infrared spectroscopy (FT IR), scanning electron microscopy with X-ray microanalysis (SEM-EDS), and X-ray powder diffraction. The antimicrobial ability of the prepared film was confirmed due to the analysis of antibacterial assay results by using gram-positive and gram-negative bacteria. Based on the achieved data, the prepared multifunctional bio-film could be a considerable candidate in packing industry, especially in medical compounds, tissue engineering, and pharmacology. پرونده مقاله