In this research, to identify the trace amount of doxorubicin, a syringe-to-syringe dispersive liquidphase microextraction (SS-DLPME) procedure combined with fluorescence spectrometry was applied. The syringe-to-syringe process was used to speed up the formation of the acceptable cloudy solution by a low volume of extraction solvent, which reduced the equilibrium time and increased the extraction efficiency. To optimize the parameters affecting the procedure, pH, type and volume of extraction solvent, the effect of salt, the number of injections, and the centrifuge speed were investigated. Under optimal conditions, the linear range of doxorubicin was found to be3.0-300.0 ng mL-1. The limit of detection, the limit of quantification, inter-day, and intra-day precision (RSD%) were 0.76, 2.55 ng mL-1, 0.82-2.11% and 1.20- 2.37%, respectively. The proposed method was successfully applied for pre-concentration and determination of the doxorubicin in urine samples. پرونده مقاله

Graphene oxide/polydopamine-polyacrylamide nanocomposite was synthesized by a simple method and used as adsorbent for dispersive micro-solid-phase extraction of phenobarbital in plasma samples. The adsorbent was characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy–energy dispersive spectroscopy and thermal gravimetric analysis. The results show that functionalization of graphene oxide by polymeric materials can enhance the sorption properties and thermal stability of the prepared adsorbent. Influential parameters on the extraction efficiency of Phenobarbital including adsorbent amount, elution solvent and its volume, sorption and desorption times and pH of sample solution were investigated and optimized. Under the optimized conditions, limits of detection and quantitation values were 1.4 and 5ng/mL, respectively. Relative recovery data for several real samples were obtained within the range of 84.0-98.0% with a relative standard deviation less than 7.2%. The proposed method was successfully applied to quantitative determination of phenobarbital in plasma samples. پرونده مقاله