In this study, the free vibration behavior of orthotropic rectangular graphene sheet embedded in an elastic medium under biaxial pre-load is studied. Using the nonlocal elasticity theory, the governing equation is derived for single-layered graphene sheets (SLGS). Differential quadrature method (DQM) has been used to solve the governing equations for various boundary conditions. To verify the accuracy of the present results, a Navier’s approach is also developed. DQM results are successfully verified with those of the Navier’s approach. The results are subsequently compared with valid result reported in the literature. The effects of the small scale, pre-load, Winkler and Pasternak foundations and material properties on natural frequencies are investigated. The results are shown that with the decrease of in-plane pre-loads the curves isotropic and orthotropic non-dimensional frequency in approaches close to each other. پرونده مقاله