Background & Aim: The present study was aimed to evaluate and compare the antibacterial activity of Schrophularia striata Boiss. andStachys schtschegleevii Sosn. Extracts against seven clinical isolates. The test isolates were Streptococcus agalactiae, Enterococcus faecalis, Staphylococcus saprophyticus, E. coli, Klebsiella pneumoniae, Proteus mirabilis and Enterobacter dissolvens. E. coli (ATCC 25922) and S.aureus (ATCC 29213) were used as quality control strains. Experimental: Clinical isolates were identified using standard microbiological methods.The methanol extracts from the aerial parts of these plants were assessed using agar well diffusion and broth microdilution methods. Considering the wide application of ciprofloxacin in treatment of Urinary Tract Infections (UTIs), susceptibility test was performed towards antibiotic. Results & Discussion: The methanol extracts of Schrophularia striata and Stachys schtschegleevii exhibit both high bacteriostatic and bactericidal activities. The Minimum Inhibitory and Bactericidal Concentration (MIC and MBC) values of Schrophularia striata extract ranged between 0.39-12.5 mg/ml and 0.78-25 mg/ml, respectively. The extract of Stachys schtschegleevii was effective in MIC and MBC ranges of 1.56-12.5 mg/ml and 3.12-50 mg/ml, respectively. On comparing the efficiency of the two extracts, Stachys schtschegleevii extract exhibited the highest activity against gram-negative bacteria, whereas, the extract of Schrophularia striata inhibited the growth of Streptococcus agalactiae and Staphylococcus spp. Industrial and practical recommendations: On considering antibacterial activity of both the extracts, these herbal extracts may treat urinary tract infections caused by some of the test isolates. پرونده مقاله