In the present work CuBi2O4 nano-spinel has been synthesized via mild hydrothermal method at 180°C for 10 h. The synthesized nanomaterials were characterized by several techniques to emphasis the structure and properties of produced materials. The crystal structure was investigated by X-ray powder diffraction method and the values of refined unit cell volume and the structure properties were studied by using the Rietveld analysis is done using fullprof program. The results shew the formation of tetrahedral structure with space group P4/ncc for this sample. Also, the morphologies of the synthesized materials were figured out by field emission scanning electron microscope (FE-SEM). According to the FESEM images, several nano cubic form particle grew on micro spherical particles. As well, the catalytic performance of obtained CuBi2O4 was studied in Biginelli reaction. The reaction conditions of this study optimized by experimental design method. This experiment stablished high catalytic performance of copper bismuth oxide in compare with some other metal oxide catalysts.Also, the results shew this product is reusable homogenous catalyst. پرونده مقاله