Introduction: The researchers believe that muscle growth and hypertrophy are influenced by different factors of myogenin and myostatin. The purpose of this study was to investigate the effect of running in shallow water, deep water and land on serum levels of myostatin and myogonin in young men. Methods: In this quasi-experimental study, nine young men were selected and ran for 24 minutes in shallow water, deep water and land in three different days with one-week interval. Blood samples were taken in three steps, before, immediately after and 8 hours after each training session. To analyze the findings of the research, Kolmogorov-Smirnov tests, two-way analysis of variance along with Bonferroni's post hoc test were used. (p≤0.05). Results: Immediately after running and eight hours after running, serum levels of myogenin increased and serum levels of myostatin decreased (p≤0.05). Nevertheless, myogenin and myostatin changes in different running conditions and at different periods were not significant (p≥0.05). Conclusion: It seems that one session of running in shallow water, deep water and land can increase serum levels of myogenin and decrease serum levels of myostatin in young men. Also, running in shallow water, deep water and land has the same effects on the increase of myogenin and the reduction of myostatin. پرونده مقاله