BACKGROUND: Nitrogen is a crucial component of plant nutrition, and its deficiency limits productivity of crops more than any other element. Organic fertilizers have an effect on the quantity and quality of carbon and soil capacity in storing and releasing nutrients needed for plant growth during process of decomposition and mineralization. OBJECTIVES: This study was done to assessment effect of different levels of amino acid and Nitrogen macro nutrient on seed yield and its components of Wheat crop. METHODS: Current research was carried out via factorial experiment based on randomized complete blocks design (RCBD) with three replications along 2022-2023 agronomic year. The treatments included Amino acid (nonuse or control, 1.5, 3 and 4.5 L.ha-1) and Nitrogen fertilizer from urea source (100% equal 300 kg.ha-1, 70% equal 210 kg.ha-1 and 40% equal 120 kg.ha-1). RESULT: Result of analysis of variance revealed effect of different level of Amino acid, Nitrogen fertilizer and interaction effect of treatments (instead harvest index) on all studied characteristics was significant. Assessment mean comparison result of interaction effect of treatments on all measured traits indicated the maximum amount of number of spike per m-2 (390.5), number of seed per spike (41.30), 1000 seed weight (42.75 g), seed yield (460.70 g.m-2) and biologic yield (1377.44 g.m-2) were noted for 4.5 lit.ha-1 amino acid and 100% Nitrogen (Also it doesn’t had significant differences with 70% Nitrogen) and lowest amount of mentioned traits belonged to nonuse of amino acid and 40% Nitrogen treatment. CONCLUSION: The use of foliar application of amino acids along with nitrogen chemical fertilizer can reduce the consumption of nitrogen chemical fertilizer in addition to producing sufficient crops, which significantly contributes to the health of the environment and is an important strategy in the direction of moving towards sustainable agriculture. Therefore, by using 70% of urea fertilizer along with 4.5 liters per hectare of amino acid, it is possible to save the consumption of chemical fertilizers and produce economic yield in studied region. پرونده مقاله