A novel soft polycaprolactone-alginate nanofiber plasma-modified with sufficient cell attachment for tissue engineering
محورهای موضوعی : Journal of NanoanalysisElham Azizifard 1 , َAzadeh Asefnejad 2 , Sedigheh Joughehdoust 3 , Hadi Baharifar 4
1 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
کلید واژه: Sodium alginate, Wound, Tissue Engineering, Polycaprolactone, Light microscopy,
چکیده مقاله :
Degradable polymers belonging to the aliphatic polyester family are currently the most attractive group of synthetic polymers. Natural and synthetic materials used in tissue engineering scaffolds should have properties such as proper biocompatibility and biodegradability with controllable degradation and adsorption rate. Synthetic polymers provide the mechanical support required by the system and the tensile strength for cell attachment and growth. Compared to synthetic polymers, natural polymers are more compatible and reduce the likelihood of tissue rejection after transplantation. In this article, sodium alginate (SA), polyvinyl alcohol (PVA) and polycaprolactone (PCL) were used to produced porous scaffold. For this purpose, different percentages of SA and PVA were prepared for electrospinning technique. The PCL/80PVA: 20SA scaffold was evaluated by fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), light microscopy (LM), swelling, biodegradability and biocompatibility analyzes after plasma process. Morphological examination showed the fiber diameter was about 299 nm and the inflation and degradation were reported at 92% and 18%, respectively. The contact angle created is equal to 42 °and the biocompatibility study for the scaffold showed 93% survival rate. The obtained results showed that PCL/80PVA: 20SA scaffold after plasma can be used in tissue engineering.
Degradable polymers belonging to the aliphatic polyester family are currently the most attractive group of synthetic polymers. Natural and synthetic materials used in tissue engineering scaffolds should have properties such as proper biocompatibility and biodegradability with controllable degradation and adsorption rate. Synthetic polymers provide the mechanical support required by the system and the tensile strength for cell attachment and growth. Compared to synthetic polymers, natural polymers are more compatible and reduce the likelihood of tissue rejection after transplantation. In this article, sodium alginate (SA), polyvinyl alcohol (PVA) and polycaprolactone (PCL) were used to produced porous scaffold. For this purpose, different percentages of SA and PVA were prepared for electrospinning technique. The PCL/80PVA: 20SA scaffold was evaluated by fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), light microscopy (LM), swelling, biodegradability and biocompatibility analyzes after plasma process. Morphological examination showed the fiber diameter was about 299 nm and the inflation and degradation were reported at 92% and 18%, respectively. The contact angle created is equal to 42 °and the biocompatibility study for the scaffold showed 93% survival rate. The obtained results showed that PCL/80PVA: 20SA scaffold after plasma can be used in tissue engineering.
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