بررسی فیتوشیمیایی و آنتیاکسیدانی عصاره میوه گیاه دارویی سنجدL. Elaeagnus angustifolia در رویشگاههای مختلف منطقه شاهرود
محورهای موضوعی : گیاهان داروییاسماعیل باباخانزاده سجیرانی 1 , سید جواد موسوی زاده 2 , خدیجه مظفری 3
1 - دانشگاه آزاد اسلامی، واحد تهران شمال، باشگاه پژوهشگران جوان و نخبگان، تهران، ایران
2 - استادیار گروه علوم باغبانی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران.
3 - کارشناسی ارشد باغبانی،دانشگاه آزاد اسلامی واحد دامغان
کلید واژه: آنتیاکسیدان, فنل و فلاونویید, سنجد (Elaeagnus angustifolia L.), رویشگاههای مختلف شاهرود,
چکیده مقاله :
گیاه سنجد (Elaeagnus angustifolia L.) به علت وجود متابولیتهای ثانوی فلاونوئید و فنول از خواص آنتی اکسیدانی برخوردار است و به این جهت دارای عملکردهای دارویی فراوان است. از آنجایی که کمیت و کیفیت آن مواد در شرایط آب و هوایی مختلف متغیر میشود، این تحقیق با هدف بررسی تغییرات فیتوشیمیایی و آنتیاکسیدانی عصاره میوههای گیاه سنجد در رویشگاههای مختلف منطقه شاهرود (مجن، میامی و کلامو 1000 - 1400 متر) انجام گرفت. عصاره متانولی میوهها با استفاده از روش خیساندن، میزان فنل و فلاونویید کل در عصارهها با استفاده از روشهای اسپکتروفوتومتری، ارزیابی و مقایسه عملکرد آنتیاکسیدانی با استفاده از روش DPPH انجام گرفت . نتایج نشان داد با افزایش ارتفاع در منطقه کوهستانی مجن (1400 متر)، میزان ترکیبات فنولی، فلاونوییدی و فعالیت آنتیاکسیدانی میوه گیاه افزایش معنیداری پیدا میکند و این موضوع در تایید اثر متقابل تنشهای محیطی موجود در ارتفاعات مختلف رویشگاهی، بر کمیت و کیفیت مواد موثره دارویی گیاهی قابل بحث است.
Elaeagnus angustifolia L. is one of the most important medicinal fruits which had a lot of secondary metabolites such as phenols and flavonoid and has been used in traditional medicine. The presences of flavonoids, phenols and antioxidant properties have been reported in fruits of Elaeagnus angustifolia L. In this research the ripe fruits of plant were collected from different regions of Shahrood (1000-1400m). The methanol extracts were obtained by maceration, total phenol and flavonoids contents were measured by spectrophotometery and antioxidant activities were analyzed by DPPH method. The results were showed that in higher region (1400m) the fruit extract of plant had the greatest amount of total phenol, flavonoids and antioxidant activity. So we observed that there is the correlation between altitude ot the region with quality and quantities of medicinal metabolites and antioxidant properties.
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