اثر محلول پاشی متانول بر برخی خصوصیات بیوشیمیایی و فیزیولوژیکی سویا (Glycine max L.) تحت تنش خشکی
محورهای موضوعی : ژنتیکبهزاد امرایی 1 , فرزاد پاک نژاد 2 , محمدعلی ابراهیمی 3 , حمید سبحانیان 4
1 - گروه زیست‌شناسی دانشگاه پیام نور، ایران
2 - گروه زراعت دانشگاه آزاد اسلامی واحد کرج، ایران
3 - گروه بیوتکنولوژی دانشگاه پیام نور، ایران
4 - گروه زیست‌شناسی دانشگاه پیام نور، ایران
کلید واژه: پراکسید هیدروژن, تنش خشکی, پرولین, ترکیبات فنلی, پروتئین کل, متانول, محتوای رطوبت نسبی,
چکیده مقاله :
به‌منظور بررسى اثرمحلول پاشى متانول وتنش خشکى بر برخى صفات بیوشیمیایی سویا (Glaycine max L.) آزمایشى گلدانی در محیط مزرعه انجام گرفت. آزمایش به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار انجام شد. تیمار‌های مورد بررسی شامل سه سطح آبیاری نرمال (آبیاری پس از 40 درصد تخلیه رطوبت قابل دسترس خاک)، تنش متوسط (آبیاری پس از 60 درصد تخلیه رطوبت قابل دسترس خاک) و تنش شدید (آبیاری پس از 70 درصد تخلیه رطوبت قابل دسترس خاک) به‌عنوان عامل اصلی و سطوح متانول به صورت محلول پاشی برگی شامل محلول‌های شاهد( محلول پاشی آب بدون مصرف متانول) و محلول‌های 7، 14 و 21 درصد حجمی متانول به عنوان عامل فرعی بود. نتایج تحقیق نشان داد بین سطوح مختلف متانول اختلاف معنى دارى در میزان کلروفیل و ترکیبات کاروتنوئیدی، محتوای رطوبت نسبی، ترکیبات فنلی، مقدار پروتئین کل، پرولین و میزان پراکسید هیدروژن برگ در سطح احتمال خطای یک درصد مشاهده شد. با شروع تنش رطوبتی از تنش ملایم تا تنش شدید کاربرد متانول 14درصد تاثیر بیشتری بر میزان کلروفیل کل، کلروفیل a، کلروفیل b، داشت. همچنین در شرایط تنش ملایم و تنش شدید با کاربرد بیشترین مقدار از سطح متانول مقدار تولید پراکسید هیدروژن به کمترین میزان رسید و مقدار ترکیبات فنلی با افزایش کاربرد متانول از 7 به 14درصد افزایش یافت. طبق نتایج به‌دست آمده با کاربرد 14 درصد متانول افزایش بیشتری در کارایی پرولین در شرایط تنش شدید مشاهده شد. با افزایش کاربرد حجمی متانول از 7 به 14 درصد مقدار آب نسبی بیشتر در گیاه در شرایط تنش حفظ شد. در مورد پروتئین با شدت یافتن تنش خشکی، تاثیر کاربرد متانول 14درصد در مقایسه با متانول 21 درصد به یک میزان بود. بنابراین با توجه به نتایج به دست آمده می توان نتیجه گرفت که متانول مقاومت گیاه در برابر تنش خشکی را بهبود بخشید.
In order to investigate the effect of foliar application of methanol and drought stress on some biochemical properties of soybean (Glaycine max L.), a pot experiment was done in farm environment. The experiments were done in factorial form based on a completely random design with 3 repetitions. Treatments included three irrigation levels of normal (irrigation after 40% depletion of available soil moisture), average stress (irrigation after 60% depletion of available soil moisture), and severe stress (irrigation after 70% depletion of available soil moisture) as the main factor and levels of methanol in the form of foliar application including control solutions (foliar application without use of methanol) and solutions of 14.7% and 21% of methanol as secondary factor. Results obtained from the study showed that there were significant differences between various levels of methanol in content of chlorophyll and carotenoid compounds, relative water content, phenolic compounds, total protein content, proline, and leaf peroxide hydrogen in (P≤0.01). With the application of stress from mild to severe, application of 14% methanol showed more pronounced effects on total chlorophyll content, chlorophyll a and chlorophyll b. Moreover, under mild and severe stress conditions, with application of the highest value of methanol, production of peroxide hydrogen reached lowest level and the content of phenolic compounds increased with the increased application of methanol from 7% to 14%. According to the obtained results, with application of 14% methanol, more increase was observed in the efficiency of proline under severe stress conditions. Increasing the volume of methanol from 7 to 14%, the relative water content was preserved under stress conditions. For protein, with increased drought stress, the effect of application of 14% methanol was the same in comparison with 21% methanol. Therefore, according to the obtained results, it is concluded that methanol could improve plant resistance against drought stress.
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