In vitro behavior of silk fibroin-coated calcium magnesium silicate scaffolds

محورهای موضوعی : Finite Element Modeling

Masoud Hafezi ¹ (Nanotechnology and Advanced Materials Division, Materials and Energy Research Center, Iran)
Hossein Mohammadi ² (School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Penang, Malaysia)
Ali Nadernezhad ³ (Biomaterials Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Alborz, Iran)
Pardis Fazlali ⁴ (Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur)
Noor Azuan Abu Osman ⁵ (Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur)

تاریخ دریافت : 1394/11/21 تاریخ پذیرش : 1394/12/22 تاریخ انتشار : 1394/12/11

کلید واژه: coating, In vitro, Scaffold, Calcium magnesium silicate, Silk,

چکیده مقاله :

Bioceramic scaffolds such as silicate bioceramics have been widely used for bone tissue engineering. However, their high degradation rate, low mechanical strength and surface instability are main challenges compromising their bioactivity and cytocompatibility which further negatively affect the cell growth and attachment. In this study, we have investigated the effects of silk fibroin coating on the tricalcium magnesium silicate scaffolds in term of biological behavior for bone tissue engineering. The microstructure, morphology, cell adhesion and chemical composition of coated scaffolds were analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy. Also, MTT assay test showed that both coated and uncoated scaffolds supported growth of mouse embryonic fibroblast cell. However, the coated scaffold revealed a higher cell proliferation than uncoated one. All the results postulated that silk fibroin was successfully coated on the scaffold and improved the biological properties of scaffold indicating a promising biomaterial for bone tissue engineering application.

منابع و مأخذ:

[1] P.V. Giannoudis, H. Dinopoulos, E. Tsiridis, "Bone substitutes: An update", Injury, Vol. 36 Suppl 3, No.  2005, pp. S20-7.

[2] R. Dimitriou, E. Jones, D. McGonagle, P.V. Giannoudis, "Bone regeneration: Current concepts and future directions", BMC med., Vol. 9, No.  2011, pp. 66.

[3] S. Ni, J. Chang, L. Chou, W. Zhai, "Comparison of osteoblast-like cell responses to calcium silicate and tricalcium phosphate ceramics in vitro", J Biomed. Mater. Res. Part B, Vol. 80B, No. 1, 2007, pp. 174-183.

[4] P. Kasten, R. Luginbühl, M. van Griensven, T. Barkhausen, C. Krettek, M. Bohner, U. Bosch, "Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, β-tricalcium phosphate and demineralized bone matrix", Biomaterials, Vol. 24, No. 15, 2003, pp. 2593-2603.

[5] Y. Zhu, C. Wu, Y. Ramaswamy, E. Kockrick, P. Simon, S. Kaskel, H. Zreiqat, "Preparation, characterization and in vitro bioactivity of mesoporous bioactive glasses (mbgs) scaffolds for bone tissue engineering", Microporous Mesoporous Mater., Vol. 112, No. 1–3, 2008, pp. 494-503.

[6] S.N. Khan, E. Tomin, J.M. Lane, "Clinical applications of bone graft substitutes", The Orthopedic clinics of North America, Vol. 31, No. 3, 2000, pp. 389-98.

[7] K. Rezwan, Q.Z. Chen, J.J. Blaker, A.R. Boccaccini, "Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering", Biomaterials, Vol. 27, No. 18, 2006, pp. 3413-3431.

[8] M. Hafezi-Ardakani, F. Moztarzadeh, M. Rabiee, A.R. Talebi, "Synthesis and characterization of nanocrystalline merwinite (ca3mg(sio4)2) via sol–gel method", Ceram. Int., Vol. 37, No. 1, 2011, pp. 175-180.

[9] M. Hafezi-Ardakani, F. Moztarzadeh, M. Rabiee, A.R. Talebi, M. Abasi-shahni, F. Fesahat, F. Sadeghian, "Sol-gel synthesis and apatite-formation ability of nanostructure merwinite (ca3mgsi2o8) as a novel bioceramic", J. Ceram. Process. Res., Vol. 11, No.  2010, pp. 765-768.

[10]  J. Ou, Y. Kang, Z. Huang, X. Chen, J. Wu, R. Xiao, G. Yin, "Preparation and in vitro bioactivity of novel merwinite ceramic", Biomed. mater. (Bristol, England), Vol. 3, No. 1, 2008, pp. 015015.

[11]  J. Zhao, Z. Zhang, S. Wang, X. Sun, X. Zhang, J. Chen, D.L. Kaplan, X. Jiang, "Apatite-coated silk fibroin scaffolds to healing mandibular border defects in canines", Bone, Vol. 45, No. 3, pp. 517-527.

[12]  H.J. Kim, U.-J. Kim, G.G. Leisk, C. Bayan, I. Georgakoudi, D.L. Kaplan, "Bone regeneration on macroporous aqueous-derived silk 3-d scaffolds", Macromol. Biosci., Vol. 7, No. 5, 2007, pp. 643-655.

[13]  C. Wu, Y. Zhang, Y. Zhu, T. Friis, Y. Xiao, "Structure–property relationships of silk-modified mesoporous bioglass scaffolds", Biomaterials, Vol. 31, No. 13, 2010, pp. 3429-3438.

[14]  M. Hafezi, M. Abbasi-shahnib, A. Zamanian, S. Hesaraki, "Preparation and characterization of whitlockite-merwinite nanocomposite", J. Ceram. Process. Res., Vol. 14, No. 1, 2013, pp. 96-99.

[15]  M. Hafezi, N. Nezafati, A. Nadernezhad, M. Yasaei, A. Zamanian, S. Mobini, "Effect of sintering temperature and cooling rate on the morphology, mechanical behavior and apatite-forming ability of a novel nanostructured magnesium calcium silicate scaffold prepared by a freeze casting method", J. Mater. Sci., Vol. 49, No. 3, 2014, pp. 1297-1305.

[16]  U.-J. Kim, J. Park, H. Joo Kim, M. Wada, D.L. Kaplan, "Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin", Biomaterials, Vol. 26, No. 15, 2005, pp. 2775-2785.

[17]  I.C. Um, H. Kweon, Y.H. Park, S. Hudson, "Structural characteristics and properties of the regenerated silk fibroin prepared from formic acid", Int. J. Biol. Macromol., Vol. 29, No. 2, 2001, pp. 91-97.

[18]  G.M. Nogueira, A.C. Rodas, C.A. Leite, C. Giles, O.Z. Higa, B. Polakiewicz, M.M. Beppu, "Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material", Bioresour. Technol., Vol. 101, No. 21, 2010, pp. 8446-8451.

[19] 

17

M. Li, W. Tao, S. Kuga, Y. Nishiyama, "Controlling molecular conformation of regenerated wild silk fibroin by aqueous ethanol treatment", Polym. Adv. Technol., Vol. 14, No. 10, 2003, pp. 694-698.

[20]  H.J. Kim, U.-J. Kim, G. Vunjak-Novakovic, B.-H. Min, D.L. Kaplan, "Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells", Biomaterials, Vol. 26, No. 21, 2005, pp. 4442-4452.

[21]  S.-M. Lien, L.-Y. Ko, T.-J. Huang, "Effect of pore size on ecm secretion and cell growth in gelatin scaffold for articular cartilage tissue engineering", Acta Biomater., Vol. 5, No. 2, 2009, pp. 670-679.

[22]  F. Shang, L. Ming, Z. Zhou, Y. Yu, J. Sun, Y. Ding, Y. Jin, "The effect of licochalcone a on cell-aggregates ecm secretion and osteogenic differentiation during bone formation in metaphyseal defects in ovariectomized rats", Biomaterials, Vol. 35, No. 9, 2014, pp. 2789-2797.

[23]  I.D. Xynos, A.J. Edgar, L.D.K. Buttery, L.L. Hench, J.M. Polak, "Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor ii mrna expression and protein synthesis", Biochem. Biophys. Res. Commun., Vol. 276, No. 2, 2000, pp. 461-465.

[24]  C. Wu, Y. Ramaswamy, A. Soeparto, H. Zreiqat, "Incorporation of titanium into calcium silicate improved their chemical stability and biological properties", J. Biomed. Mater. Res. Part A, Vol. 86A, No. 2, 2008, pp. 402-410.

[25]  A. John, H.K. Varma, T.V. Kumari, "Surface reactivity of calcium phosphate based ceramics in a cell culture system", J. Biomater. Appl., Vol. 18, No. 1, 2003, pp. 63-78.

[26]  C. Wu, Y. Ramaswamy, Y. Zhu, R. Zheng, R. Appleyard, A. Howard, H. Zreiqat, "The effect of mesoporous bioactive glass on the physiochemical, biological and drug-release properties of poly(dl-lactide-co-glycolide) films", Biomaterials, Vol. 30, No. 12, 2009, pp. 2199-2208.

[27]  Q. Lu, X. Hu, X. Wang, J.A. Kluge, S. Lu, P. Cebe, D.L. Kaplan, "Water-insoluble silk films with silk i structure", Acta Biomater., Vol. 6, No. 4, 2010, pp. 1380-1387.

[28]  Q. Lu, X. Wang, S. Lu, M. Li, D.L. Kaplan, H. Zhu, "Nanofibrous architecture of silk fibroin scaffolds prepared with a mild self-assembly process", Biomaterials, Vol. 32, No. 4, 2011, pp. 1059-1067.

[29] 

18

D.W. Hutmacher, "Scaffolds in tissue engineering bone and cartilage", Biomaterials, Vol. 21, No. 24, 2000, pp. 2529-2543.

[30]  S.I. Roohani-Esfahani, Z.F. Lu, J.J. Li, R. Ellis-Behnke, D.L. Kaplan, H. Zreiqat, "Effect of self-assembled nanofibrous silk/polycaprolactone layer on the osteoconductivity and mechanical properties of biphasic calcium phosphate scaffolds", Acta Biomater., Vol. 8, No. 1, 2012, pp. 302-312.