تأثیر پرایمینگ تغذیه ای بذر با سولفات آهن و سولفات روی بر خصوصیات جوانه‌زنی و رشد گیاهچه بذرهای عدس
محورهای موضوعی : فیزیولوژی و متابولیسم بذر
محمد وحدانی رشوانلویی
1
,
مجید جامی الاحمدی
2
,
محمد حسن سیاری زهان
3
,
هادی شوریده
4
,
مسلم مصطفایی
5
1 - دانشجوی کارشناسی ارشد، گروه مهندسی تولید و ژنتیک گیاهی، دانشگاه بیرجند
2 - استاد گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه بیرجند
3 - دانشیار گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه بیرجند
4 - استادیار. مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان شمالی
5 - دانشجوی دکتری فیزیولوژی گیاهان زراعی، گروه مهندسی تولید و ژنتیک گیاهی، دانشگاه بیرجند
کلید واژه: پیش‌تیمار تغذیه‌ای, تقویت بذر, سرعت جوانه‌زنی, عناصر ریزمغذی,
چکیده مقاله :
در یک مطالعه آزمایشگاهی، اثر پیش‌تیمار تغذیه‌ای بذر بر صفات مربوط به جوانه‌زنی و رشد گیاهچه عدس (Lens culinaris Med.) در قالب دو آزمایش جداگانه در قالب طرح کاملاً تصادفی با سه تکرار برای دو ماده پیش‌تیمار {پرایم با سولفات آهن (FeSO4) و سولفات روی (ZnSO4)} بررسی شد. در هر آزمایش و برای هر ماده پرایم، پنج سطح از غلظت ماده پرایم (30، 60، 90، 120 و 150 میلی مولار) در نظر گرفته شد. صفات مورداندازه‌گیری عبارت بودند از درصد و سرعت جوانه‌زنی، طول ریشه‌چه و ساقه‌چه وزن تر و خشک گیاهچه و شاخص بنیه طولی گیاهچه. نتایج داده‌ها نشان داد که از نظر نوع ماده پیش‌تیمار، سولفات آهن نسبت به سولفات روی برتری نشان داد و باعث بهبود غالب شاخص‌های جوانه-زنی، به‌استثنای سرعت جوانه‌زنی، شد. همچنین با افزایش غلظت از 30 به 60 میلی‌مولار، بالاترین شاخص‌های جوانه زنی و رشد و بنیه گیاهچه به دست آمد. هرچند با افزایش بیشتر غلظت ماده پیش‌تیمار ، یک روند کاهشی در تمامی صفات اندازه-گیری مشاهده شد که می‌تواند احتمالاً به دلیل ایجاد سمیت در بذرهای تحت غلظت‌های بالای فلزات باشد. در کل با توجه به نتایج کسب‌شده، به نظر می‌رسد استفاده از این دو ماده، به‌ویژه سولفات آهن، حداکثر با غلظت 60 میلی‌مولار برای بهبود ویژگی‌های جوانه‌زنی و تغذیه‌ای بذرهای سودمند باشد.
In a laboratory study, the effect of seed nutritional pretreatment on germination and seedling growth traits of lentil (Lens culinaris Med.) in the form of two separate experiments in the form of a completely randomized design with three replications for two pretreatment materials [prime with iron sulfate (FeSO4) and zinc sulfate (ZnSO4) ] were investigated. In each experiment and for each prime material, five levels of prime material concentration (30, 60, 90, 120 and 150 mM) were considered. The measured traits were germination percentage and speed, length of root and shoot, wet and dry weight of seedling, and longitudinal root index of seedling. The results of the data showed that in terms of the type of pretreatment, iron sulfate was superior to zinc sulfate and caused the major improvement of the germination indicators, except for the germination speed. Also, by increasing the concentration from 30 to 60 mM, the highest indicators of germination and seedling growth were obtained. However, with a further increase in the concentration of the pre-treatment material, a decreasing trend was observed in all the measured traits, which could possibly be due to the toxicity in the seeds under high concentrations of metals. In general, according to the obtained results, it seems that the use of these two substances, especially iron sulfate, with a maximum concentration of 60 mM, is beneficial for improving the germination and nutritional characteristics of seeds.
Abdoli, M., and Esfandiari, E. 2014. Effect of zinc foliar application on the quantitative and qualitative yield and seedlings growth characteristics of bread wheat (cv. Kohdasht). Iranian Dryland Agronomy Journal. 3(1): 77-90. (in Persian with English abstract). https://doi.org/10.22092/idaj.2014.100557
Abdul-Baki, A.A., and Anderson, J.D. 1973. Vigor Determination in Soybean Seed by Multiple Criteria. Crop Science 13: 630-633. https://doi.org/10.2135/cropsci1973. 0011183X001300060013x
Aboutalebian, M.A., and Mohagheghi, A. 2015. Influence of different seed priming treatments on yield and yield components of lentil under terminal drought stress condition. Journal of Crop Production and Processing 5(15):129-141. (in Persian with English abstract). https://doi.org/10.18869/acadpub.jcpp.5.15.129
Adhikari, T., Kundu, S., and Rao, A.S. 2016. Zinc delivery to plants through seed coating with nano-zinc oxide particles. Journal of Plant Nutrition. 39(1): 136-146 https://doi.org/10.1080/01904167.2015.1087562
Afzal, S., Sharma, D., and Singh, N.K. 2021. Eco-friendly synthesis of phytochemical-capped iron oxide nanoparticles as nano-priming agent for boosting seed germination in rice (`ryza sativa L.). Environmental Science and Pollution Research. 28(30): 40275-40287. https://doi.org/10.1007/s11356-020-12056-5
Ahmadi, K., Parsa, S., Mahmoodi, S., and Gazanchian, G. 2016. Evaluation of effect of nutrient priming on the Galbanum (Ferula gummosa Boiss.) germination and seedling growth. Seed Ecophysiology Journal. 1(2): 137-151. (in Persian with English abstract). https://doi.org/10.22077/sej.2016.426
Al-Mudaris, M.A., and Jutzi, S.C. 1999. The Influence of Fertilizer-based Seed Priming Treatments on Emergence and Seedling Growth of Sorghum bicolor and Pennisetum glaucum in Pot Trials under Greenhouse Conditions. Journal of Agronomy and Crop Science 182: 135-142. https://doi.org/10.1046/j.1439-037x.1999.00293.x
Ameri, A., Fatemi, H., Aroiee, H., and Teixeira da Silva, J.A. 2011. What's the Effect of Saline Priming on Germination Factors of Capsicum annuum var.'California Wonder'Seeds. Seed Science and Biotechnology. 5(1): 47-49.
Arif, M., Ali, S., Shah, A., Javad, N. and Rashid, A. 2005. Seed priming maize for improving emergence and seedling growth. Sarhad Journal of Agriculture. 21: 539-543.
Arif, M., Waqas, M., Nawab, K., and Shahid. M., 2007. Effect of seed priming in Zn solutions on chickpea and wheat. African Crop Science Conference Proceedings. 8: 237-240.
Asgedom, H., and Becker, M. 2001. Effects of seed priming with nutrient solutions on germination, seedling growth and weed competitiveness of cereals in Eritrea. In: Proceeding of Deutscher Tropentag 2001, University of Bonn and ATSAF, Margraf Publishers Press, Weickersheim, 282p.
Atar, B., Uygur, V., and Sukuşu, E. 2020. Effects of priming with copper, zinc and phosphorus on seed and seedling composition in wheat and barley. Turkish Journal of Agricultural and Natural Sciences. 7(1): 104-111. https://doi.org/10.30910/turkjans.680021
Badiri, A. 2013. The effect of seed priming with micronutrient elements on the germination indicators, growth and yield of the medicinal plant plantain. MSc. Thesis. Islamic Azad University, Karaj Branch, Karaj, Iran. (In Persian).
Bradford, J. K. 1995. Water relations in seed germination. In: J. Kigel and G. Galili (Eds.).Seed development and germination. Marcel Dekker, New York. pp. 351-396.
Cakmak, I. 2008. Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant and Soil. 302: 1-17. https://doi.org/10.1007/s11104-007-9466-3
Erskine, W., Sarker, A., and Kumar, S. 2011. Crops that feed the world 3. Investing in lentil improvement toward a food secure world. Food Security. 3: 127–139. https://doi.org/10.1007/s12571-011-0124-5
Farooq, M., Wahid, A., and Siddique, K.H. 2012. Micronutrient application through seed treatments – a review. Journal of Soil Science and Plant Nutrition 12(1): 125-142. https://doi.org/10.4067/S0718-95162012000100011
Ghaderifar, F., Kamkar, B., and Soltani, A. 2014. Seed Science and Technology. Jahad University Press of Mashhad, 512 p. (In Persian).
Gorzi, A., Omidi, H., and Bostani, A. 2020. Effect of Stevia (Stevia rebaudiana) Seed Priming Treatments with Salicylic Acid, Iron, and Zinc on Some Germination Traits and Photosynthetic Pigments under Drought Stress. Iranian Journal of Seed Research. 6(2): 125-135. (in Persian with English abstract). https://doi.org/10.29252/yujs.6.2.125
Harris, D., Rashid, A., Miraj, G., Arif, M., and Shah, H. 2007. ‘On-farm’ seed priming with zinc sulphate solution—A cost-effective way to increase the maize yields of resource-poor farmers. Field Crops Research. 102(2): 119-127. https://doi.org/10.1016/j.fcr.2007.03.005
Hoseinpur Askarian, E., Abbasi Surki, A., Danesh Shahraki, A. 2019. Effect of Seed Priming with ZnSO4 and FeSO4 on Dormancy Break Optimization and Germination Traits of Shallot (Allium hirtifolium). Iranian Journal of Seed Research. 6(1): 33-49. (in Persian with English abstract). https://doi.org/10.29252/yujs.6.1.33
Imran, M., Neuman, G., and Rombeld, V. 2008. Nutrient seed priming improves germination rate and seedling growth under subemergence stress at low temperature. In: Proceedings of Tropentag 2008, Competition for Resources in a Changing World: New Drive for Rural Development. October 7-9, 2008. Hohenheim, Germany.
Johnson, S., Layren, J., Welch, R., and Duxbury, J. 2005. A comparison of the effects of micronutrient seed priming and soil fertilization on the mineral nutrition of chickpea (Cicer arietinum), Lentil (Lens culinaris), rice (Oryza sativa) and wheat (Triticum aestivum) in Nepal. Experimental Agriculture. 41(4): 427-448. https://doi.org/10.1017/ S0014479705002851
Jokar, L., Ronaghi, A., Karimian, N., and Ghasemi-Fasaei, R. 2015. Effects of different Fe levels from Fe-nano-chelate and Fe-EDDHA sources on growth and some nutrients concentrations in cowpea in a calcareous soil. Journal of Soil and Plant Interactions 6(2): 9-19. (in Persian with English abstract). https://doi.org/10.18869/acadpub.ejgcst.6.2.9
Kamkar, B., Safahani Langeroudi, A.R., and Mohammadi, R. 2012. The Use of Nutrients in Crop Plants. Jahad University Press of Mashhad, Iran. 500 p. (In Persian).
Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop Science. 2: 176-177. https://doi.org/10.2135/ cropsci1962.0011183X000200020033x
Makkizadeh Tafti, M., Tavakol Afshari, R., Majnoon Hosseini, N., Naghdi Badi, H., and Mehdizadeh, A. 2006. Effect of Osmopriming on Seed Germination of Borage (Borago officinalis L.). Iranian Journal of Medicinal and Aromatic Plants Research. 22(3): 216-222. (in Persian with English abstract).
Malakouti, M.J., Keshavarz, P., and Karimian, N. 2008. A Comprehensive Approach towards Identification of Nutrient Deficiencies and Optimal Fertilization for Sustainable Agriculture. 7th ed. Tarbiat Modares University Publication. Tehran, Iran. (in Persian).
Marschner, P. 2012. Marschner's Mineral Nutrition of Higher Plants. Third Edition. Academic Press, London. https://doi.org/10.1016/C2009-0-63043-9
Mirshekari, B. 2015. Effects of seed priming with microelements of Fe and B on some germination parameters and yield of marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants Research, 30(6): 879-888. https://doi.org/10.22092/ijmapr.2015.11923
Mirshekari, B., 2013. Effect of seed priming with microelements on germination speed, seedling vigor and flower yield of marigold (Calendula officinalis L.). Agroecology Journal. 9(4): 69- 76. (in Persian with English abstract).
Mohammad, W., Shah, S.M., Nawas, H., and Iqbal, M.M. 1999. Interactive effect of nitrogen, zinc and boron on yield and nutrient uptake by rapeseed. Pakistan Journal of Soil Science. 16(1-2): 111-114.
Ozturk, L., Yazici, M.A., Yucel, C., Torun, A., Cekic, C., Bagci, A., Ozkan, H., Braun, H.-J., Sayers, Z. and Cakmak, I. 2006. Concentration and localization of zinc during seed development and germination in wheat. Physiologia Plantarum. 128: 144-152. https://doi.org/10.1111/j.1399-3054.2006.00737.x
Prasad, A.S. 2003. Zinc deficiency. British Medical Journal 326: 409-410. https://doi.org/10.1136/bmj.326.7386.409
Rahimi, M., Heidari, M., and Eftekhari, S.A. 2012. Effects of Iron sulphate and iron chelate on seed germination and early seedling growth of Vigna unguiculata. 1st National Conference on Agriculture in Hard Environmental Conditions, May 9, 2012. Islamic Azad University, Ramhormoz Branch, Iran. (in Persian with English abstract).
Shinde, P., Doddagoudar, S.R., Vasudevan S.N. 2016. Influence of seed polymer coating with micronutrients and foliar sprayon seed yield of chickpea (Cicer arietinum L.). Legume Research. 40(4): 704-709. https://doi.org/10.18805/lr.v0iOF.10760
Soltani, A., Gholipoor, M., and Zeinali, E. 2001. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55(1): 195-200. https://doi.org/10.1016/j.envexpbot.2004.10.012
Tahmasebi, R., Sajedi, N.A., and Shoaei, S. 2017. Evaluation effect of different solutions and seed priming treatments on germination, agronomic and quality characteristics of red bean genotypes. Iranian Journal Pulses Research. 8(1): 60-72. (in Persian with English abstract). https://doi.org/10.22067/ijpr.v8i1.26145
Vojodi, M.L., Hassanpouraghdam, M.B., Ebrahimzadeh, A., and Valizadeh, K.R. 2016. Effects of ZnSO4 foliar application on vegetative growth and phenolic and essential oil content of geranium (Pelargonium odoratissimum L.). Journal of Ornamental Plants. 6: 193-199.
