The effects of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)
محورهای موضوعی : Nano Biophotonics
Habib Tajalli
1
(Biophotonics Research Center,Tabriz Branch, Islamic Azad University)
Masoud Maleki
2
(Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran)
Esmail Safavi
3
(Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Azad Islamic University, Tabriz, Iran)
Reza Shahi
4
(Department of Mathematics and Statistics, Tabriz branch, Islamic Azad University, Tabriz, Iran)
Fatemeh Firoozi
5
(Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran)
Zahra Akbarpour
6
(Department of Basic Science, Biotechnology Research Center, Tabriz Branch, Azad Islamic University, Tabriz, Iran)
Alireza Sotoudeh Khyaban
7
(Research Center of Biophtonics, Islamic Azad university, Tabriz Branch, Tabriz, Iran)
کلید واژه: Infertility, Spermatogenesis, azoospermia, Laser therapy,
چکیده مقاله :
In this paper we investigate the effect of low power laser on spermatogenesis in testicular tissue of azoospermia mouse model in-vivo. In this experimental work, 112 adult male Syrian mice were randomly divided into three main groups: negative control group, positive (Azoospermia control) group, and experimental group, but to determine the best dose of laser radiation three experimental groups were tested. To create azoospermia control group, Busulfan was used at a dose of 30mg/kg, for 21 days by intraperitoneal injection. In the experimental groups after Busulfan treatment, they were applied by the low power diode laser (wavelength of 808nm) with three different energy densities of 2, 4, and 8 J/cm2. The employment of a laser with an energy density of 8 J/cm2 was shown to be beneficial in boosting germ cell and sperm production.
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