ارزیابی تاثیر اسید¬آمینه متیونین بر شاخص¬های حرکتی اسپرم اپیدیدیمی بز طی ماندگاری در دمای ¬5¬ درجه سلسیوس با استفاده از سیستم رایانه¬ای CASA

محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی

مهران لطفی ¹ , کیوان عبدی ²

1 - دانش‌آموخته دامپزشکی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.
2 - استادیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران.

تاریخ دریافت : 1402/07/05 تاریخ پذیرش : 1403/11/06 تاریخ انتشار : 1403/05/30

کلید واژه: حرکت اسپرم, اپیدیدیم, بز, متیونین, نرم افزار کاسا.,

چکیده مقاله :

ذخیره اسپرم به صورت مایع،  با اکسیداسیون چربی­های غشاء و تولید گونه­های اکسیژن واکنشی (reactive oxygen species; ROS) الگوی حرکتی اسپرم  را مختل می­سازد. اسیدآمینه متیونین به عنوان آنتی‌اکسیدان، در پاکسازی ROS و بهبود تحرک اسپرم موثر است. هدف از انجام تحقیق حاضر، بررسی تاثیر 4 غلظت متیونین بر الگوی حرکتی اسپرم اپیدیدیمی بز طی ماندگاری در دمای 5 درجه سلسیوس بود. به این منظور 30 جفت بیضۀ بز نر بالغ از کشتارگاه صنعتی ارومیه جمع­آوری و کنار یخ خشک به آزمایشگاه منتقل گردید. پس از بذل دم اپیدیدیم، اسپرم­ها به میکروتیوب­های حاوی محیط ­HTF (Human Tubal Fluid)­، با 10 درصد آلبومین سرم گاوی منتقل شد. پس از تهیه رقت 30 الی 40 میلیون اسپرم در میلی لیتر محیط HTF، غلظت­های 1، 2، 4 و 6 میلی­مول متیونین به اسپرم اضافه گردید. در زمان­های 1، 12، 24، 48 و 60 ساعت بعد از نگه‌داری در دمای 5 درجه سلسیوس، نمونه­ها پس از 5 دقیقه گرمخانه‌گذاری در دمای 37 درجه سلسیوس، از نظر الگوی حرکتی با سیستم رایانه­ای CASA (Computer Assisted Sperm Analysis) ارزیابی گردید. داده­ها با استفاده از نرم­افزار SPSS و انجام آنالیز آماری با آزمون توکی، ثبت گردید. نتایج نشان داد که شاخص­های کاسا مثل حرکت پیش­رونده، سرعت خط مستقیم، متوسط سرعت مسیر حرکت، سرعت خط منحنی، مستقیم­الخط بودن و درصد لرزش مسیر حرکت، در غلظت 2 میلی­مول متیونین و در زمان 60 ساعت، به طور معنی­داری از شاهد بیشتر بوده است (p<0/05). بنابراین، طبق نتایج به­دست­آمده می­توان از اسید آمینه متیونین در بهبود کیفیت اسپرم اپیدیدیمی بز بهره جست.

 

چکیده انگلیسی:

Sperm storage in liquid form disrupts sperm motility by oxidation of membrane lipids and production of reactive oxygen species (ROS). The amino acid methionine as an anti-oxidant is effective in clearing ROS and improving sperm motility. The aim of this study was to investigate the effect of four concentrations of methionine on the motility pattern of goat epididymal sperm during preservation at 5 °C. For this purpose, 30 pairs of testicles of adult male goats were collected from Urmia industrial slaughterhouse and transferred to the laboratory next to ice pack. In the laboratory, after incisions on the epididymal tail, sperm were transferred to micro-tubes containing HTF (Human Tubal Fluid) medium with 10% bovine serum albumin. After preparing a dilution between 30 and 40 million sperm per ml of HTF medium, 4 levels of methionine (1, 2, 4 and 6 mmol) were added to the micro-tubes containing culture medium and sperms. After 5 minutes of incubation at 37 °C, the samples were evaluated in terms of motility pattern with computer assisted sperm analysis (CASA). Data were analyzed using SPSS software and the Tukey test. The results showed that CASA parameters including progressive motility (class A+B, %), straight line velocity (VSL, µm/s), average path velocity (VAP, µm/s), curvilinear velocity (VCL, µm/s), straightness (STR, %) and wobble (WOB, %) were significantly higher in methionine treatment at 60 hours than in the control (p<0.05). Therefore, methionine amino acid can be used to improve the quality of goat epididymis sperm.

 

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