جداسازی کلستریدیوم پرفرینجنس تیپA از شیردان 3 رأس بره مبتلا به التهاب شیردان
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
مصطفی عبداللهی
1
,
غلامرضا عبداله پور
2
,
ایرج اشرافی تمای
3
,
جمشید رزمیار
4
,
امیر اصغری باغخیراتی
5
1 - دستیار تخصصی گروه بیماری‌های داخلی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
2 - استاد گروه بیماری‌های داخلی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
3 - کارشناس آزمایشگاه، گروه میکروبیولوژی و ایمنولوژی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
4 - دانشیار گروه بیماری‌های پرندگان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
5 - دستیار تخصصی گروه بیماری‌های پرندگان، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران.
تاریخ دریافت : 1400/05/31
تاریخ پذیرش : 1400/10/19
تاریخ انتشار : 1400/08/01
کلید واژه:
کلستریدیوم پرفرینجنس,
بره,
التهاب شیردان,
چکیده مقاله :
در اسفند ماه سال 1398، سه رأس بره 5 تا 15 روزه نژاد مخلوط به بیمارستان دامپزشکی دانشگاه تهران ارجاع داده شدند. شکایت دامدار از وجود تلفات 20 درصدی (42 رأس) در بره های 4 تا 17 روزه گله خود که تنها با شیر مادر تغذیه می شدند، بود. در مشاهده و معاینه بالینی، زمین گیری، افسردگی، ضعف، دمای 40 درجه سلسیوس، اتساع شیردان با مایعات، تاکی پنه، تاکیکاردی و پرخونی مخاطات ثبت گردید. بره ها پس از معاینات بالینی، تحت بررسی خو ن شناسی قرارگرفته و سپس در شرایط بهداشتی کالبدگشایی شدند. همچنین خون، مغز استخوان، مغز، ریه، قلب، کبد، کلیه، روده و شیردان مورد کشت های باکتریایی هوازی و بی هوازی قرار گرفتند. در بررسی خون شناسی، آنمی نرموسیتیک نرموکرومیک، لکوپنی، لنفوپنی، انحراف به چپ، حضور شیستوسیت، کراتوسیت، میلوسیت و متامیلوسیت مشاهده شد. در کالبدگشایی، زخم های سرسنجاقی در مخاط شیردان، حضور توده های خونی شبیه به دانه قهوه در محتویات شیردان و رنگ پریدگی کلیه ها وجود داشت. در رنگ آمیزی گرم گسترش مستقیم شیردان، تعداد زیادی باکتری میله ای شکل گرم مثبت مشاهده شد. همچنین از شیردان و روده دام های بیمار، کلستریدیوم پرفرینجنس تیپA و اشریشیا کولای غیرپاتوژن جدا گردید. مطالعه حاضر نشان داد که باکتری کلستریدیوم پرفرینجنس تیپA ممکن است در بیماری التهاب شیردان بره ها دخیل باشد.
چکیده انگلیسی:
In March 2020, three 5-15 day old mixed breed lambs were referred to the University of Tehran Veterinary Hospital. The farmer complained of 20% mortality rate (42 heads) in 4-17 day old lambs of his flock that they were exclusively breastfed. In observation and clinical examination, recumbency, depression, weakness, 40 ° C temperature ,abomasal dilation with fluid, tachypnea, tachycardia and hyperemia of the mucosa were recorded. After clinical examination, the lambs were examined by hematology and then necropsied under sterile conditions. In hematologic examination, normocytic-normochromic anemia, leukopenia, lymphopenia, left shift, the presence of schistocytes, keratocytes, myelocytes and metamyelocytes were observed. In necropsy, there were abomasal petechiae hemorrhages, coffee grain-like blood masses in the contents of the abomasum and pale kidneys. The direct gram smear from the abomasum had a large number of positive gram rods. All organs were subjected to aerobic and anaerobic bacterial cultures. Clostridium perfringens type A and non-pathogenic E coli were isolated from the abomasum and intestine of patients. The highest antibiotic susceptibility of Clostridium perfringens A isolate was to ceftiofur and ceftriaxone antibiotics. This study showed that Clostridium perfringens A may be involved in the pathogenesis of abomasitis in lambs. . . . . . .
منابع و مأخذ:
Abdollahi, M., Mohammadi, H.R., Ahmadi-Hamedani, M., Abdollahi, M. and Shahbazi, V. (2019). The effect of oral administration of Folus (Cassia fistula) fruit aqueous extract on abomasal emptying rate in neonatal lambs. Journal of Veterinary Clinical Pathology, 13(49): 67-106. [In Persian]
Ahsani, M., Bafti, M.S., Esmailizadeh, A. and Mohammadabadi, M. (2011). Genotyping of isolates of Clostridium perfringens from vaccinated and unvaccinated sheep. Small Ruminant Research, 94(1): 65-69.
Chapman, T.A., Wu, X.Y., Barchia, I. and Bettelheim, K.A. (2006). Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine. Applied and Environmental Microbiology, 72(7): 4782-4795.
Christodoulopoulos, G., Scott, P.R., Jehl, N., Filioussis, G. and Smith, S.H. (2013). Clinical, microbiological and histological findings in lambs affected by ‘salivary abomasum disease’. Veterinary Record, 172(4): 100-105.
Goekce, H.I., Genç, O., Soezmen, M. and Gökçe, G. (2007). Determination of Clostridium perfringens toxin-types in sheep with suspected enterotoxemia in Kars Province, Turkey. The Turkish Journal of Veterinary and Animal Sciences, 31(1): 355-360.
Greco, G., Madio, A., Buonavoglia, D., Totaro, M., Corrente, M. and Martella, V. (2005). Clostridium perfringens toxin-types in lambs and kids affected with gastroenteric pathologies in Italy. The Veterinary Journal, 170(3): 346-350.
Itodo, A., Adesiyun, A., Adekeye, J. and Umoh, J. (1986). Toxin-types of Clostridium perfringens strains isolated from sheep, cattle and paddock soils in Nigeria. Veterinary Microbiology, 12(4): 93-96.
JahedDashliboron, O. and Hassanpour, A. (2013). Survey of risk factors for the prevalence of leptospiral infection in horses of Gonbad area. Journal of Veterinary Clinical Pathology, 7(26): 1844-1907. [In Persian]
Kheirkhah, B. and Hatam-Jahromi, M. (2016). Genotypic of Clostridium perfringens in cold area of Kerman province. Journal of Microbial World, 9(2): 169-175.
Khodadad, S., Mohajeri, D. and Kaffashi Elahi, R. (2018). The effect of hydroalcoholic extract of Brasscia rapa. L root on methotrexate induced hepatotoxicity in the rat. Journal of Veterinary Clinical Pathology, 12(46): 167-191. [In Persian]
Lotfollahzadeh, S., Abdollahi, M., Noshirvani, P., Mohammadi, H.R. and Abdollahi, M. (2020). Comparison of the effect of Microvit supplementation and Ferrodrop oral drop on primary parameters of hemogram and serum minerals in 20-day-old healthy and Pica affected lambs. Journal of Veterinary Clinical Pathology, 14(53): 25-35. [In Persian]
Mohammadzadeh, A., Mahmoodi, P., Ashrafi tamai, I. and Sharifi, A. (2017). Molecular analysis of virulence genes stx1, stx2, eaeA and hlyA in Escherichia coli isolated from cloacal samples in wild pigeons (Columba livia) and determination of their antibiotic resistance. Journal of Veterinary Research, 72(2): 219-225.
Poursoltani,, Mohsenzadeh, M., Razmyar, J. and Peighambari. S.Y. (2014). Toxinotyping of Clostridium perfringens Strains Isolated from Packed Chicken Portions. Iranian Journal of Medical Microbiology, 8(1): 8-17. [In Persian]
Roeder, B.L., Changappa, M.M., Nagaraja, T.G., Avery, T.B. and Kennedy, G.A. (1988). Experimental induction of abomasal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of Clostridium perfringens type A in neonatal calves. American Journal of Veterinary Research, 49(1): 201-207.
Smith, B.P. (2014). Large Animal Internal Medicine. 5th, Edinburgh: Elsevier Health Sciences. pp: 723-725.
Songer, J.G. and Miskimins, D.W. (2005). Clostridial abomasitis in calves: Case report and review of the literature. Anaerobe, 11(5): 290-294.
Timoney, J.F., Gillespie, J.H., Scott, F.W. and Barlough, J.E. (1988). Hagan and Bruner’s microbiology and infectious diseases of domestic animals. 1st, US, New York, Ithaca, Comstock Publishing Associates. pp: 43-51.
Van Kruiningen, H.J., Nyaoke, C.A., Sidor, I.F., Fabis, J.J., Hinckley, L.S. and Lindell, K.A. (2009). Clostridial abomasal disease in Connecticut dairy calves. Canadian Veterinary Journal, 50(3): 857-860.
Vatn, S. and Ulvund, M.J. (2000). Abomasal bloat, haemorrhage and ulcers in young Norwegian lambs. Veterinary Record, 146(2): 35-39.
Vatn, S., Sjaastad, O.V. and Ulvund, M.J. (2000). Histamine in lambs with abomasal bloat, haemorrhage and ulcers. Journal of Veterinary Medicine, A, Physiology, Pathology, Clinical Medicine, 47(4): 251-255.
Yadegar, F., Nakhaei, P., Hashemtabar, G., Kalidari, G., Rashtibaf, M. and Razmyar, J. (2018). Major and minor toxins of Clostridium perfringens isolated from healthy and diseased sheep. Small Ruminant Research, 168(1): 1-5.
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Abdollahi, M., Mohammadi, H.R., Ahmadi-Hamedani, M., Abdollahi, M. and Shahbazi, V. (2019). The effect of oral administration of Folus (Cassia fistula) fruit aqueous extract on abomasal emptying rate in neonatal lambs. Journal of Veterinary Clinical Pathology, 13(49): 67-106. [In Persian]
Ahsani, M., Bafti, M.S., Esmailizadeh, A. and Mohammadabadi, M. (2011). Genotyping of isolates of Clostridium perfringens from vaccinated and unvaccinated sheep. Small Ruminant Research, 94(1): 65-69.
Chapman, T.A., Wu, X.Y., Barchia, I. and Bettelheim, K.A. (2006). Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine. Applied and Environmental Microbiology, 72(7): 4782-4795.
Christodoulopoulos, G., Scott, P.R., Jehl, N., Filioussis, G. and Smith, S.H. (2013). Clinical, microbiological and histological findings in lambs affected by ‘salivary abomasum disease’. Veterinary Record, 172(4): 100-105.
Goekce, H.I., Genç, O., Soezmen, M. and Gökçe, G. (2007). Determination of Clostridium perfringens toxin-types in sheep with suspected enterotoxemia in Kars Province, Turkey. The Turkish Journal of Veterinary and Animal Sciences, 31(1): 355-360.
Greco, G., Madio, A., Buonavoglia, D., Totaro, M., Corrente, M. and Martella, V. (2005). Clostridium perfringens toxin-types in lambs and kids affected with gastroenteric pathologies in Italy. The Veterinary Journal, 170(3): 346-350.
Itodo, A., Adesiyun, A., Adekeye, J. and Umoh, J. (1986). Toxin-types of Clostridium perfringens strains isolated from sheep, cattle and paddock soils in Nigeria. Veterinary Microbiology, 12(4): 93-96.
JahedDashliboron, O. and Hassanpour, A. (2013). Survey of risk factors for the prevalence of leptospiral infection in horses of Gonbad area. Journal of Veterinary Clinical Pathology, 7(26): 1844-1907. [In Persian]
Kheirkhah, B. and Hatam-Jahromi, M. (2016). Genotypic of Clostridium perfringens in cold area of Kerman province. Journal of Microbial World, 9(2): 169-175.
Khodadad, S., Mohajeri, D. and Kaffashi Elahi, R. (2018). The effect of hydroalcoholic extract of Brasscia rapa. L root on methotrexate induced hepatotoxicity in the rat. Journal of Veterinary Clinical Pathology, 12(46): 167-191. [In Persian]
Lotfollahzadeh, S., Abdollahi, M., Noshirvani, P., Mohammadi, H.R. and Abdollahi, M. (2020). Comparison of the effect of Microvit supplementation and Ferrodrop oral drop on primary parameters of hemogram and serum minerals in 20-day-old healthy and Pica affected lambs. Journal of Veterinary Clinical Pathology, 14(53): 25-35. [In Persian]
Mohammadzadeh, A., Mahmoodi, P., Ashrafi tamai, I. and Sharifi, A. (2017). Molecular analysis of virulence genes stx1, stx2, eaeA and hlyA in Escherichia coli isolated from cloacal samples in wild pigeons (Columba livia) and determination of their antibiotic resistance. Journal of Veterinary Research, 72(2): 219-225.
Poursoltani,, Mohsenzadeh, M., Razmyar, J. and Peighambari. S.Y. (2014). Toxinotyping of Clostridium perfringens Strains Isolated from Packed Chicken Portions. Iranian Journal of Medical Microbiology, 8(1): 8-17. [In Persian]
Roeder, B.L., Changappa, M.M., Nagaraja, T.G., Avery, T.B. and Kennedy, G.A. (1988). Experimental induction of abomasal tympany, abomasitis, and abomasal ulceration by intraruminal inoculation of Clostridium perfringens type A in neonatal calves. American Journal of Veterinary Research, 49(1): 201-207.
Smith, B.P. (2014). Large Animal Internal Medicine. 5th, Edinburgh: Elsevier Health Sciences. pp: 723-725.
Songer, J.G. and Miskimins, D.W. (2005). Clostridial abomasitis in calves: Case report and review of the literature. Anaerobe, 11(5): 290-294.
Timoney, J.F., Gillespie, J.H., Scott, F.W. and Barlough, J.E. (1988). Hagan and Bruner’s microbiology and infectious diseases of domestic animals. 1st, US, New York, Ithaca, Comstock Publishing Associates. pp: 43-51.
Van Kruiningen, H.J., Nyaoke, C.A., Sidor, I.F., Fabis, J.J., Hinckley, L.S. and Lindell, K.A. (2009). Clostridial abomasal disease in Connecticut dairy calves. Canadian Veterinary Journal, 50(3): 857-860.
Vatn, S. and Ulvund, M.J. (2000). Abomasal bloat, haemorrhage and ulcers in young Norwegian lambs. Veterinary Record, 146(2): 35-39.
Vatn, S., Sjaastad, O.V. and Ulvund, M.J. (2000). Histamine in lambs with abomasal bloat, haemorrhage and ulcers. Journal of Veterinary Medicine, A, Physiology, Pathology, Clinical Medicine, 47(4): 251-255.
Yadegar, F., Nakhaei, P., Hashemtabar, G., Kalidari, G., Rashtibaf, M. and Razmyar, J. (2018). Major and minor toxins of Clostridium perfringens isolated from healthy and diseased sheep. Small Ruminant Research, 168(1): 1-5.
