تاثیر هشت هفته تمرین تناوبی و ژل رویالn کروموزومی بر آنزیم های کبدی و شاخص مقاومت به انسولین رت های چاق دیابتی نوع دو
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستیسحر ریاستی 1 , حسین عابدنطنزی 2 , ماندانا غلامی 3
1 - دانشجوی دکتری تخصصی فیزیولوژی ورزشی، گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - استادیار، گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - گروه تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: تمرین تناوبی شدید, ژل رویال , آنزیم کبدی, دیابت نوع دو, موش ,
چکیده مقاله :
زمینه و هدف : دیابت نوع دو شایع¬ترین بیماری درون¬ریز است که به¬دلیل عدم تحمل گلوکز دراثر برهم¬خوردن تعادل بین ذخایر و تقاضای انسولین رخ می¬دهدکه با تغییرات ساختاری و عملکردی در کبد منجر به تغییرات در آنزیم های کبدی می شود. هدف پژوهش حاضر مطالعه تغییرات آنزیم های کبدی و شاخص مقاومت ¬به¬انسولین پس از انجام تمرین تناوبی و ژل رویال در رت¬های چاق دیابتی نوع دو بود. مواد و روش‌ها: نمونه آماری پژوهش حاضر 36 سر موش¬های صحرایی نر چاق با میانگین وزن 409 گرم بودند. پس از20 هفته تغذیه با رژیم پرچرب با تزریق درون صفاقی 25 میلی‌گرم STZ به ازای کیلوگرم وزن موش‌ها دیابتی شدند. موش‌هایی با گلوکز ناشتای آنها بین150 تا400 میلی‌گرم‌دردسی‌لیتر، دیابتی نوع دوم در نظر گرفته شد. موش‌های دیابتی در 4 گروه کنترل (6 سر)، تمرین تناوبی (8 سر)، ژل رویال (7 سر) ، تمرین تناوبی شدید- ژل رویال ( 8 سر) گروه بندی و پروتکل تمرینی و گاواژ ژل رویال روی آنها به مدت 8 هفته اجرا شد. هشت هفته تمرین تناوبی شدید ، پنج جلسه در هفته با تناوب شدید 2 دقیقه‌ای80 تا 90 درصد VO2max و تناوب استراحت یک دقیقه‌ای با 50 تا 56 درصدVO2max اجرا شد. ژل رویال بصورت گاواژ به میزان 100 میلی گرم برکیلوگرم 5 روز در هفته داده شد. پس از پایان پروتکل خونگیری انجام شد و آنزیم های کبدی و گلوکز با استفاده از اتوآنالیزر و انسولین با استفاده از کیت آزمایشگاهی اندازه گیری و با استفاده از آزمون تحلیل واریانس یکطرفه و دوعاملی و آزمون تعقیبی تجزیه و تحلیل شدند. نتایج: نشان داد در مقایسه با گروه کنترل، تمرین تناوبی شدید به کاهش معنی¬دار گلوکز و شاخص مقاومت به انسولین و تمرین تناوبی به کاهش آنزیم های کبدی ALT و AST و ALP در مقایسه با گروه کنترل منجر شد که در مورد ALP کاهش معنی دار بود (P=0.001). نتیجه گیری: به نظر می‌رسد تمرین تناوبی شدید و مصرف ژل رویال ، بتوانند درکاهش آنزیم‌های کبدی و بهبود وضعیت کبد در موش‌های صحرایی تاثیر گذار باشند.
Background & Aim: Type 2 diabetes is the most common endocrine disease that occurs due to glucose intolerance due to imbalance between reserves and insulin demand, which leads to be changes in liver enzymes with structural and functional changes in the liver. The aim of this study was to study changes in liver enzymes and insulin resistance index after HIIT and royal jelly in obese rats with type 2 diabetes. Materials & Methods: The statistical sample of the present study was 36 obese male rats with an average weight of 409 gr. After 20 weeks of high-fat diet, rats became diabetic by intraperitoneal injection of 25 mg/kg STZ body weight. Mice with fasting glucose between 150 and 400 mg /dl were considered to have type 2 diabetes. Diabetic rat in 4 groups of control (N=6), HIIT (N=8), royal gel (N=7), HIIT& Royal gel (N=8) group done and Accomplished Eight weeks of HIIT, 5d/w with intense 2-minute intervals of 80 to 90% VO2max and one-minute rest intervals with 50 to 56% VO2max. Royal jelly was given by gavage at the rate of 100 mg / kg 5 days a week. At the end of the protocol, blood samples were taken and liver enzymes and glucose were measured using an auto-analyzer and insulin was measured using a laboratory kit and analyzed using one-way and two-way analysis of variance and post hoc test. Results: showed that in comparison with the control group, HIIT led to a significant decrease in glucose and insulin resistance index and intermittent exercise resulted in a decrease in liver enzymes ALT, AST and ALP compared to the control group. The ALP decrease was significant (P = 0.001). Conclusion: It seems that HIIT and consumption of royal jelly can help reduce liver enzymes and improve liver status in rats.
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