اثر محلول پاشی نانو دی اکسید تیتانیوم و سالیسیلیک اسید بر برخی ویژگی های بیوشیمیایی و تولید دانه ذرت سینگل کراس 704 تحت رژیم های آبیاری
محورهای موضوعی :
اکوفیزیولوژی گیاهان زراعی
فائزه شرقی
1
(فرهیخته ی کارشناسی ارشد زراعت، دانشکده کشاورزی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران)
ابراهیم خلیلوند بهروزیار
2
(استادیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران)
تاریخ دریافت : 1397/11/16
تاریخ پذیرش : 1398/02/25
تاریخ انتشار : 1398/09/20
کلید واژه:
پرولین,
پراکسیداز,
گایاکول پراکسیداز,
پلی فنل اکسیداز,
کربوهیدرات های محلول,
چکیده مقاله :
به‌منظور بررسی اثر محلول پاشی نانو ذرات دی اکسید تیتانیوم و سالیسیلیک اسید بر برخی از ویژگی های بیوشیمیایی و تولید دانه ذرت هیبرید سینگل کراس 704 تحت رژیم های آبیاری، آزمایشی به صورت اسپلیت پلات فاکتوریل در قالب طرح بلوک های کامل تصادفی در سه تکرار در ایستگاه تحقیقاتی دانشکده کشاورزی دانشگاه آزاد اسلامی واحد تبریز در سال زراعی 1397-1396 به اجرا در آمد. عامل اصلی تنش کمبود آب در سه سطح 50، 75 و 100 درصـد رطوبت قابل دسترس و ترکیب فاکتوریلی کاربـرد نانو دی اکسید تیتانیوم (n-TiO2) (در سه سطح صفر، 01/0 و 03/0 درصد) و سالیسیلیک اسید (SA) (در دو سطح صفر و نیم درصد) به عنوان عامل فرعی در نظر گرفته شدند. بیشترین فعالیت آنزیم پراکسیداز، پلی فنل اکسیداز و کمترین فعالیت مالون دی آلدئید در اثر محلول پاشی با 5/0 درصد SA در شرایط آبیاری 50 درصد رطوبت قابل دسترس بود. محلول پاشی با 01/0 درصد TiO2بیشترین فعالیت آنزیم پراکسیداز و کمترین فعالیت مالون دی آلدئید را به خود اختصاص داد. در شرایط کاربرد و عدم کاربرد SA، محلول پاشی با 01/0 درصد TiO2تاثیر مثبتی بر فعالیت آنزیم پلی فنل اکسیداز داشت. محلول پاشی با 01/0 درصد TiO2و 5/0 درصد SA تحت رطوبت 50 درصد رطوبت قابل دسترس نیز بیشترین مقدار کربوهیدرات های محلول و پرولین را داشت. بر اساس نتـایج با افزایش سطح تنش کمبود آب فعالیت آنزیم گایاکول پراکسیداز نیـز افزایش یافت به طوری که در آبیاری 50 درصد رطوبت قابل دسترس بیشترین فعالیت این آنزیم مشاهده شد. با توجه به نقش صفات مورد مطالعه در همکاری با یکدیگر در ممانعت از تولید گونه های فعال اکسیژن و کاهش اثرات مخرب تنش کمبود آب، کابرد سالیسیلیک اسید و نانو دی اکسید تیتانیوم با افزایش فعالیت آنزیم پراکسیداز، پلی فنل اکسیداز، مقدار کربوهیدرات های محلول، پرولین همزمان با کاهش میزان مالون دی آلدئید موجب کاهش اثرات منفی تنش کمبود آب گردید. با توجه به این که نانو ذرات دی اکسید تیتانیوم و اسید سالیسیلیک بر آنزیم های تاثیرگذار بر فیزیولوژی گیاه اثرات مثبتی داشتند از این رو میزان تولید دانه ذرت نسبت به شاهد و شرایط کم آبیاری افزایش قابل ملاحظه ای نشان داد.
چکیده انگلیسی:
In order to investigate the effects of water deficit stress and nano-TiO2 and salicylic acid foliar application on some biochemical traits of corn704 single crossplant, an experiment was conducted in split plot factorial based on RCBD in three replications at the Research Station of the Islamic Azad University, Tabriz Branch, during growing seasons of 2017-2018. Treatments were water deficit stress in three levels contained: 50, 75 and 100% filed capacity (FC) as well as thefactorial combination of nano-TiO2 (n-TiO2) foliar application in three levels contains: non application (control), 0.01 and 0.03 and salicylic acid (SA) foliar application in two levels contain: non application and 0.5%. Result showed that 0.5% SA foliar application under 50% water deficit stress had the highest peroxidase, polyphenol oxidase and lowest malondialdehyde activity. Furthmore, 0.01% n-TiO2 foliar application had the highest effect on peroxidase and lowest effect on malondialdehyde activity. Use and non-use condition of SA, 0.01% n-TiO2 foliar application had the positive effect on polyphenol oxidase activity. Foliar application of 0.01% n-TiO2 and0.5% SA under water deficit stress had the highest effect on soluble carbohydrates and proline. Based on the results, the guaiacol peroxidase enzyme activity increased with increasing water deficit stress level, so that the most activity of this enzyme was observed in irrigation with 50 % filed capacity. Considering the role of the traits studied in cooperation with each other in preventing the production of active oxygen species and reducing the effects of water deficit stress, SA and n-TiO2, increasing the activity of enzyme peroxidase, polyphenol oxidase, soluble carbohydrates, Proline and in contrast to the reduction of malondialdehyde reduced the negative effects of water deficit stress. Since SA and n-TiO2 had positive effects on the enzymes affecting plant physiology, the seed yield increased significantly compared to control and water deficiet conditions.
منابع و مأخذ:
· Abdel Latef, A.A.H., A.K. Srivastava, M.S., Abd El-Sadek, M. Kordrostami, and L.P. Tran. 2017. Titanium dioxide nanoparticles improve growth and enhance tolerance of broad bean plants under saline soil conditions. Land Degradation and Development. 29(4): 1065-1073.
· Abedi, T., and H. Pakniyat. 2012. Antioxidant enzyme changes in response to drought stress in ten cultivar of oil seed rap (Brassica napus L.). Genetics and Plant Breeding. 46(4): 27-34.
· Aldesuquy, H.S., M.A. Abass, S.A. Abo-Hamed, and A.H. Elhakem. 2013. Does glycine betaine and salicylic acid ameliorate the negative effect of drought on wheat by regulating osmotic adjustment through solutes accumulation? Journal of Stress Physiology and Biochemistry. 9(3): 5-22.
· Amirjani, M. R., M. Askari, and P. Askari. 2014. The effect nano oxide zinc on the amount of alkaloids, enzymatic and nonenzymatic antioxidants and some indicators of physiology Catharantus roseu. Cells and Tissues Journal. 5(2): 173-183. (In Persian).
· Amiri, A., S.R. Parsa, M. Nezami, and A. Ganjeali. 2011. The effects of drought stress at different phonological stages on growth indices of chickpea (Cicer arietinum L.) in greenhouse conditions. Pulses Research 1: 69-84. (In Persian).
· Aref, I., H. El Atta, M. El Obeid, A. Ahmed, P. Khan, and M. Iqbal. 2013. Effect of water stress on relative water and chlorophyll contents of Juniperus procera Hochst. ex Endlicher in Saudi Arabia. Life Science Journal. 10(4): 681–685.
· Ashraf, M., and A. Iram. 2005. Drought stress induced changes in some organic substances in nodules and other plant parts of two potential legumes differing in salt tolerance. Flora. 200: 446-535
· Azarpanah, A., O. Alizadeh, and H. Dehghanzadeh. 2013. Investigation on proline and carbohydrates accumulation in Zea mays L. under water stress condition. Extreme life, biospeology and asterobiology. International Journal of the Bioflux Society. 5 (1): 47-54.
· Blum, A. 2017 Osmotic adjustment is a prime drought stress adaptive engine in support of plant production: Osmotic adjustment and plant production. Plant Cell Environment. 40, 4–10.
· Bundig, C., T.H. Vu, P. Meise, S. Seddig, A. Schum, T. Winkelmann. 2016. Variability in Osmotic Stress Tolerance of Starch Potato Genotypes (Solanum tuberosum L.) as Revealed by an In Vitro Screening: Role of Proline, Osmotic Adjustment and Drought Response in Pot Trials. Joyrnal of Agronomy and Crop Science. 203, 206–218.
· Daneshmand, F. 2014. Response of antioxidant system of tomato to water deficit stress and its interaction with ascorbic acid. Iranian Journal of Plant Biology. 6(19): 57-72. (In Persian).
· Dazy, M., V. Jung, J. Ferard, and J. Masfaraud. 2008. Ecological recovery of vegetation on a coke-factory soil: Role of plant antioxidant enzymes and possible implication in site restoration. Chemosphere. 74: 57-63.
· Dubios, M., K.A. Gilles, J.K. Hamilton, P.A. Rebers, and F.S. Ith.1956. Calorimetric method for determination of sugars and related substances. Chemosphere. 28: 350- 356.
· Ghanati, F., A. Morita, and H. Yokota. 2002. Induction of suberin and increase of liginin content by exess boron in Tabacco cell. Soil Science, Plant Nutrition. 48(3): 357-364.
· Gregersen, P.L., A. Culetic, L. Boschian, and K. Krupinska. 2013. Plant senescence and crop productivity. Plant Molecular Biology. 82: 603–622.
· Hashemi-Dehkourdi, E., M. Mousavi, N. Moallemi, and M.H. Ghafariyan-Moghareb. 2017. Effect of nanoparticles of titanium dioxide (anatase) on physiological characteristics of strawberry (Fragaria ananassa c.v.Queen Elisa) in hydroponic condition. Journal of Plant Process and Function. 5(16): 1-8. (In Persian).
· Hamidzadeh Moghadam, S.H., S. Jahanbakhsh Godekahriz, and A. Ebadi. 2015. Salicylic acid effect on variation of carbohydrate content, total protein and antioxidant enzymes during programmed cell death in two wheat cultivars. Journal of Crop Production. 7(2): 97-112. (In Persian).
· Hayat, S., and A. Ahmad. 2007. Salicylic acid: A plant hormone. Springer, Dordrecht, Netherlands Pp: 97-99
· Heath, R.L., and L. Packer. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125: 189–198.
· Hemeda, H.M., and B.P. Klein. 1990. Effects of naturally occurring antioxidants on peroxidase activity of vegetable extracts. Journal of Food Science. 55: 184–185.
· Israr, M., and S.V. Sahi. 2006. Antioxidative responses to mercury in the cell cultures of Sesbania drummondii. Plant Physiology Biochemical. 44: 590-595
· Hong, F., J. Zhou, C. Liu, F. Yang, C. Wu, L. Zheng, and P. Yang. 2005. Effects of nano TiO2 on photochemical reaction of chloroplasts of Spinach. Journal Biological Trace Element Research. 105: 269-279.
· Jaberzadeh, A., Moaveni, P., Tohidi Moghadam, H., Moradi, A., 2010. Effect of nano titanium dioxide particles spraying on some agronomy traits of wheat under drought stress condition. Crop Ecophysiology. 2(4), 259-301. (In Persian).
· Khalilvand Behrouzyar, E. 2017. Effect of seed priming with ethanol, methanol, boron and manganese on some of morpho-physiological characteristics of rapeseed (Brassica napus L.) under water deficit stress. Journal of Crop Ecophysiology. 11(4): 805-820. (In Persian).
· Khan, M.N., M. Mobin, Z.K. Abbas, K.A. AlMutairim, and Z.H. Siddiqui. 2017. Role of nanomaterials in plants under challenging environments. Plant Physiology and Biochemistry. 110: 194–209.
· Khater, M.S. 2015. Effect of titanium nanoparticles (TiO2) on growth, yield and chemical constituents of coriander plants. Arab Journal of Nuclear Science and Applications. 48(4): 187-194
· Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. International Journal of Agriculture and Biology. 6: 5-8.
· Lang-Mladek, C., O. Popova, K. Kiok, M. Berlinger, B. Rakic, and W. Aufastez. 2010. Transgenerational inheritance and resetting of stress-induced loss of epigenetic in Arabidopsis. Molecular Plant. 3: 594-602.
· Lei, Z., S. Mingyu, W. Xiao, L. Chao, Q. Chunxiang, C. Liang, H. Hao, L. Xiao-qing, and H. Fashui. 2008. Antioxidant stress is promoted by nano-anatase in spinach chloroplasts under UV-B radiation. Biological Trace Element Research. 121: 69–79
· Luxmore, B. 1990. Methods of soil Analysis. Part II, 3th Edition, pp.493-59.
· Mohammadi, H., M. Esmailpour, and A. Gheranpaye. 2017. Effects of TiO2 nanoparticles and water deficit stress on morpho-physiological characteristics of dragonhead (Dracocephalum moldavica L.) plants. Acta Agriculturae Slovenica. 107(2): 385-396.
· Mohammadi, M., and H. Kazemi. 2002. Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science. 162: 491-498.
· Moussa, H., and S.M. Abdel-Aziz. 2008. Comparative response of drought tolerant and drought sensitive maize genotypes to water stress. Australian Journal of Crop Science. 1: 31-36.
· Mazarie, A., S.M. Mousavi-nik, A. Ghanbari, and L. Fahmideh. 2019. Effect of different spraying concentrations of jasmonic acid and titanium dioxide nanoparticles on some physiological traits and antioxidant system activity of Sage (Salvia officinalis L.). Iranian Journal of Plant Biology. 11(1): 1-22. (In Persian).
· Newman, S., D. Tantasawat, and J. Steffens. 2011. Tomato polyphenol oxidase B is spatially and temporally regulated during development. Molecules. 6: 493-517
· Rao, S.R., A. Qayyum, A. Razzaq, M. Ahmad, I. Mahmood, and A. Sher. 2012. Role of foliar application of salicylic acid and L-Tryptophan in drought tolerance on maize. The Journal of Animal and Plant Sciences. 22(3): 768-772.
· Rosi, H.S. 2018. Kalyanasundaram, synthesis, characterization, structural and optical properties of titanium-dioxide nanoparticles using Glycosmis cochinchinensis leaf extract and its photocatalytic evaluation and antimicrobial properties. World News of Natural Sciences. 17: 1-15.
· Sheraphti chaleshtari, F., R. Sheraphti chaleshtari, and M. Momeni. 2008. The antimicrobial effects of aqueous extract and ethanol plant Scrophularia striata on E. coli in Laboratory. Journal of Medical Sciences (ShaherKord University). 10(4): 32-37. (In Persian).
· Shoghiyan, M., and A. Rozbahani. 2017. The effect of salicylic acid foliar application on morphological traits, yield and yield components of red bean under drought tension conditions. Crop Physiology Journal. 34(9): 131-147. (In Persian).
· Soltani, M., P. Moaveni, and H. Noori. 2013. The effect of foliar application of nanoparticles of titanium dioxide on yield and antioxidant enzyme activities in lentil (Lens culinaris Medik.). Journal of Plant Ecophysiology Research, Special Issue, 78-88. (In Persian)
· Sugio, A., R. Dreos, F. Aparicio, and A.J. Maule. 2009. The cytosolic protein response as a subcomponent of the wider heat shock response in Arabidopsis. Plant Cell. 21: 642-645
· Sunkar, R. 2010. Plant stress tolerance methods and protocols, Humana Press, Oklahoma.
· Wang, L.J., and S.H.H. Li. 2006. Salicylic acid induced heat or cold tolerance in relation to Ca2+ homeostasis and antioxidant system in young grape plants. Plant Science. 170: 685-694.
· Yang, F., F. Hong, W. You, C. Liu, F. Gao, C. Wu, and P. Yang. 2006. Influence of nanoanatase TiO2 on the nitrogen metabolism of growing spinach. Biological Trace Element Research. 110(2): 179-190
· Zheng, L., M. Su., C.H. Liu, C. Li, H. Huang, X. Wu, X. Liu, X. Yang., F. Gao, and F. Hong. 2007. Effects of nanoanatase TiO2 on photosynthesis of spinach chloroplasts under different light Illumination. Biological Trace Element Research. 119: 68–76.
· Zlatev, Z., and F.C. Lidon. 2012. An overview on drought induced changes in plant growth, water relations and photosynthesis. Emirates Journal of Food and Agriculture. 24: 57-72.
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