Growth and Biochemical Properties of Green Basil (Ocimum basilicum L.) Affected by Foliar Application of Biostimulants
محورهای موضوعی : Journal of Crop Nutrition ScienceMozhgan Alibakhshi 1 , Hossein Ali Asadi-Gharneh 2
1 - M.Sc. Student, Department of Horticulture, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
2 - Associated Professor, Department of Horticulture, Faculty of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
کلید واژه: Plant height, Ascorbic acid, Carotenoid, <i>Leaf area, Total phenolic content</i>,
چکیده مقاله :
BACKGROUND: Green basil is a nutritional and medicinal plant due to its valuable properties. Improving qualitative and quantitative traits of plants with appropriate methods in sustainable agricultural were emphases. Biostimulants derived from organic materials and stimulate natural process in plants and improve crop performance. OBJECTIVES: In this research the effects of some biostimulants (amino acids, humic acid and seaweed extract) on morpho-physiological properties of green basil were evaluated. METHODS: This research was carried out under greenhouse condition. The experiment was conducted based on Completely Randomized Design with ten treatments and three replications. The treatments included control (distilled water), seaweed extract (0.5, 1 and 2 gr.l-1), amino acid (1, 2 and 3 gr.l-1) and humic acid (1, 2 and 3 ml.l-1). RESULT: The results showed that application of different biostimulants positive effects on vegetative parameters and biochemical properties of green basil plants. The highest leaf numbers (21.67) was measured in amino acid application at 1 g.l-1 concentration. The effect of humic acid was more noticeable on increased plant height (37.00- 47.67 cm) in compare to other biostimulants. The plants that received biostimulants had higher plant fresh weight than control treatment. Seaweed extract and humic acids increased significantly carotenoid content of plants. The highest ascorbic acid content was measured in treated plants at levels of 2 and 3 gr.l-1 amino acid. Application of all biostimulants were used in this study, had positive effects on concentration of total phenolic content of basil plants. CONCLUSION: According to the results of the present study, it can be concluded that, application of biostimulants at proper concentration due to positive effect on quality and quantity of growth parameters and biochemical properties of green basil plants are recommended.
Abdel-Mawgoud, A., A. Tantaway, M. M. Hafez. and H. A. Habib. 2010. Seaweed extract improves growth, yield quality of different watermelon hybrids. Res. J. Agri. Biol. Sci. 6(2): 161-168.
AOAC. Association of Official Analytical Chemists. 1984. Official Methods of Analysis of the Association of Official Analytical Chemists (14th edition). Arlington. Virginia. USA.
Azza, S. M. and R. Yousef. 2015. Response of Basil Plant (Ocimum sanctum L.) to Foliar Spray with Amino Acids or Seaweed Extract. J. Horticultural Sci. Ornamental Plants. 7(3): 94-106.
Barker, A. V. and D. J. Pilbeam. 2007. Handbook of plant nutrition. CRC Press. Coral Gables. FL.
Bashir, A., M. Rizwan, S. Ali, M. Z. Rehman, W. Ishaque, M. A. Riaz. and A. Maqbool. 2018. Effect of foliar-applied iron complexes with lysine on growth and cadmium (Cd) uptake in rice under Cd stress. Environ Sci. Pollut. Res. 25: 20691-20699.
Bhatla, S. C. and M. A. Lal. 2018. Plant physiology, development and metabolism. Springer Nature Singapore. 1251 pp.
Chanwitheesuk, A., A. Teerawutgulrag. and N. Rakariyatham. 2005. Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand. Food Chem. 92: 491–497.
Delshadi, S., M. Ebrahimi. and E. Shirmohammadi. 2017. Plant growth promoting bacteria effects on growth, photosynthetic pigments and root nutrients uptake of Avena sativa L. under drought stress. Desert. 22-1: 107-116.
Dixon, M. W. 2018. Chemical fertilizer in transformations in world agriculture and the state system, 1870 to interwar period. J. Agrar. Chang. 18: 768-786.
Du Jardin, P. 2015. Plant biostimulants: Definition, concept, main categories and regulation. Sci. Hortic. 196: 3–14. Doi: 10.1016/j.scienta.2015.09.021.
El-Afifi, S. T., M. M. Zaghloul, M.B.I. El-Sawy. and A. M. A. Hasham. 2014. Effect of starter solutions in soil and foliar spray with some stimulants on growth and productivity of Chinese cabbage. J. Plant Prod. 5(1): 21–40.
El-Sayed, A. A., S. H. El-Hanafy. and R. A. El-Ziat. 2015. Effect of chicken manure and humic acid on herb and essential oil production of Ocimum sp. Am. Eur. J. Agric. Environ. Sci. 15(3): 367-379.
Erulan, V., P. Soundarapandian, G. Thirumaran. and G. Ananthan. 2009. Studies on the effect of Sargassum Polycystum (C. Agardh, 1824) extract on the growth and biochemical composition of Cajanus Cajan (L.) Mill Sp. Am. Eur. J. Agri. Environ. Sci. 6(4): 392-399.
González, A., J. Castro, J. Vera. and A. Moenne. 2013. Seaweed oligosaccharides stimulate plant growth by enhancing carbon and nitrogen assimilation, basal metabolism, cell division. J. Plant Growth Regul. 32: 443–448.
Hatice, A. and F. Gusler. 2005. The effects of sulfur and humic acid on yield component and macronutrient contents of Spinach (Spinacia oleracea var. spinoza). Biol. Sci. 5(6): 801–804.
Hernández-Herrera, R. M., F. Santacruz-Ruvalcaba, M. A. Ruiz-Lopez, J. Norrie. and G. Hernández-Carmona. 2014. Effect of liquid seaweed extracts on growth of tomato seedlings (Solanum lycopersicum L.). J Appl. Phycol. 26: 619–628.
Huang, W. Y., Y. Z. Cai. and Y. Zhang. 2009. Natural phenolic compounds from medicinal herbs 300 and dietary plants: potential use for cancer prevention. Nutr. Cancer 62(1): 1-20.
Ji, S. H., J. S. Kim, C. H. Lee, H. S. Seo, S. C. Chun, J. Oh, E. H. Choi. and. G. Park. 2019. Enhancement of vitality and activity of a plant growth promoting bacteria (PGPB) by atmospheric pressure non-thermal plasma. Sci. Rep. 9(1044): 1-16.
Khan, W., U. P. Rayirath, S. Subramanian, M. N. Jithesh, P. Rayorath, D. M. Hodges, A. T. Critchley, J. S. Craigie, J. Norrie. and B. Prithiviraj. 2009. Seaweed extracts as biostimulants of plant growth and development. J. Plant Growth Regulation. 28(4): 386- 399.
Li, G., Sh. Wan, J. Zhou, Zh. Yang. and P. Qin. 2009. Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinus commonis L.) seedlings to salt stress levels. Industrial Crops and Products. 31: 13-19.
Lin, D., M. Xiao, J. Zhao, Z. Li, B. Xing, X. Li. and S. Chen. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules. 21(10): 1374.
Lola-Luz, T., F. Hennequart, M. Gaffney. 2013. Enhancement of phenolic and flavonoid compounds in cabbage (Brassica oleraceae) following application of commercial seaweed extracts of the brown seaweed (Ascophyllum nodosum). Agri. Food Sci. 22: 288-295.
Maach, M., K. Boudouasar, M. Akodad, A. Skalli, A. Moumen. and M. Baghour. 2020. Application of biostimulants improves yield and fruit quality in tomato. Intl. J. Vegetable Sci. https://doi.org/10.1080/19315260.2020.1780536.
Maach, M., K. Boudouasarb, M. Akodada, A. Skallia, A. Moumena. and M. Baghour. 2020. Application of biostimulants improves yield and fruit quality in tomato. Intl. J. Vegetable Sci. doi.org/10.1080/19315260.2020.1780536.
Mafakheri, S. and B. Asghari. 2018. Effect of seaweed extract, humic acid and chemical fertilizers on morphological, physiological and biochemical characteristics of Trigonella foenum-graecum L. J. Agr. Sci. Tech. 20: 1505-1516.
Mahfouz, S. and M. Sharaf-Eldin. 2007. Effect of mineral Vs. biofertilizer on growth, yield, and essential oil content of Fennel (Foeniculum Vulgare Mill.). Intl. Agrophysics. 21(4): 361-366.
Mzibra, A., A. Aasfar, M. Khouloud, Y. Farrie, R. Boulif, I. M. Kadmiri, A. Bamouh. and A. Douira. 2021. Improving growth, yield, and quality of tomato plants (Solanum lycopersicum L.) by the application of Moroccan seaweed-Based biostimulants under greenhouse conditions. Agronomy. 11: 1373.https://doi.org.10.3390/agronomy11071373.
Nardi, S., M. Schiavon. and O. Francioso. 2021. Chemical structure and biological activity of humic substances define their role as plant growth promoters. Molecules. 26: 2256.
Pise, N. M. and A. B. Sabale. 2010. Effect of seaweed concentrates on the growth and biochemical constituents of Trigonella foenum-graecum L. J. Phytol. 2(4): 50-56.
Putievsky, E. and B. Galambosi. 1999. Production systems of sweet basil. In R. Hiltunen and Y. Holm (Eds.). Basil: The genus Ocimum. Vol. 10. pp: 39–65. Amsterdam: Harwood Acad. Pub.
Poursakhi, S. R. and H. A. Asadi-Gharneh. 2019. Phytochemical responses of white savory (Satureja mutica Fisch and C. A. Mey.) to foliar application of seaweed extract and ecormon fertilizer. J. Crop. Nutr. Sci. 5(4): 23-31.
Shehata, A. M. and W. S. Nosir. 2019. Response of sweet basil plants grown under salinity stress to spraying seaweed extract. The Future J. Biol. 2(1): 16-28.
Singleton, V. L. and J. A. Rossi. 1965. Colorimetry of total phenolics phosphomolybdic phosphotungstic acid reagents. Am. J. Enol. Vitic 16: 144-158.
Thambiraj, J., K. Lingakumar. and S. Paulsamy. 2012. Effect of seaweed liquid fertilizer prepared from Sargassum wightii and Hypnea musciformis on the growth and biochemical constituents of the pulse, Cyamopsis tetragonoloba (L.). J. Agric. Res. 1: 65-70.
Vasantharaja, R., L. S. Abraham. D. Inbakandan. R. Thirugnanasambandam, T. Senthilvelan, S. A. Jabeen. and P. Prakash. 2019. Influence of seaweed extracts on growth, phytochemical contents and antioxidant capacity of cowpea (Vigna unguiculata L. Walp). Biocatal Agri. Biotechnol. 17: 589-594.