بررسی دزهای کشنده جدایه ایرانی قارچ Beauveria bassiana در کنترل میکروبی بید آرد (Lep.: Pyralidae) Ephestia kuehniella روی
محورهای موضوعی : گیاه پزشکینگار بهمنی 1 , هادی استوان 2 , مسعود لطیفیان 3 , بهار راد 4
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کلید واژه: خرما, date palm, کنترل میکروبی, بید آرد, Microbial control, flour moth,
چکیده مقاله :
بید آرد Ephestia kuehniella از آفات مهم انباری می باشد. این تحقیق به منظور بررسی کارایی جدایه ایرانی مناسب قارچ Beauveria bassiana در کنترل میکروبی شب پره آردEphestia kuehniella روی خرمای رقم سایر انجام شد. برای تعیین قدرت کشندگی قارچ روی مراحل تخم، لارو و شفیره، پنج دُز 104،104×5، 105، 105×5 و 106اسپور در میلی لیتر به روش غوطه ورسازی استفاده شد. داده ها با استفاده از روش لگاریتم-پروبیت برازش گردیدند. نتایج نشان داد که بیش ترین دُز کشنده 50 درصد مربوط به جدایه IRAN441Cروی شفیره 104 × 76/1 و کمترین دُز کشنده 50 درصد مربوط به مرحله تخم و معادل103×49/3 اسپور در میلی لیتر بود. بالاترین و پایین‌ترین زمان50 درصد کشندگی به ترتیب برای شفیره و تخم و معادل 67/3 و 38/3 روز بود. به طورکلی نتایج تحقیقات در فاز آزمایشگاهی که قدم نخست در امکان‌سنجی کاربرد این قارچ در کنترل میکروبی بید آرد می باشد، نشان داد که قارچ عامل بیمارگر از پتانسیل کاربرد بالایی برخوردار است.
The Ephestia kuehniella is one of the important pest of storage Date. This research was conducted to evaluating the efficiency the suitable Iranian isolate of Fungus Beauveria bassiana for the microbial control the flour moth on the Sayer date cultivar. The larvae were treated by 5 doses of fungi including 104,5×104,105, 5×105 and 106spores/ml to determine the lethal power, with dropping method. Data were calculated by using Log-Probit analysis methods. Results showed that the highest and lowest LC50 were for pupa and egg equivalent to 1.76×104 and 3.49×103 spores/ml respectively. The lowest and highest LT50were assessed for pupa and egg equivalent to 3.67 and 3.38 days respectively. The research results of in the laboratory phase as the first step of feasibility of fungus application in microbial control showed that fungal pathogen had high potential for application on control of flour moth in date
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