تاثیر سه ماه تمرین هوازی اینتروال و مکمل کوئرستین بر غلظت Baxو Bcl-2 در هیپوکامپ موش های صحرایی نر مدل پارکینسونی
محورهای موضوعی : فیزیولوژی تجربی و آسیب شناسی
سیروس کیان مهر
1
,
جبار بشیری
2
,
رقیه پوزش جدیدی
3
,
حسن پوررضی
4
,
میر علیرضا نورآذر
5
1 - گروه علوم ورزشي، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
3 - گروه تربیت بدنی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران
4 - فیزیولوژی ورزشی، دانشگاه بين المللي امام خميني، قزوين، ایران.
5 - دانشکده دامپزشکي، گروه علوم پايه، واحد علوم پزشکي تبريز، دانشگاه آزاد اسلامي، تبريز، ایران.
کلید واژه: تمرین هوازی اینتروال, کوئرستین, هیپوکامپ, Bax, Bcl-2,
چکیده مقاله :
زمینه و هدف: بیماری پارکینسون یک اختلال پیشرونده عصبی ناشی از مرگ نورون ها در جسم سیاه، انحطاط انتقال عصبی دوپامینرژیک و حضور آلفاسینوکلئین و اجزاء پروتئینی در جسم سلولی نورون هاست. هدف از انجام تحقیق حاضر تبیین تاثیر سه ماه تمرین هوازی اینتروال و مکمل کوئرستین بر غلظت Baxو Bcl-2 در هیپوکامپ موش های صحرایی نر مدل پارکینسونی بود.
مواد و روشها: برای انجام تحقیق آزمایشی حاضر 28 رت نر نژاد ویستار تهیه و به 5 گروه کنترل سالم 5 رت، کنترل پارکینسونی 5 رت، تمرین پارکینسونی: 6 رت ، ملاتونین پارکینسونی: 6 رت و تمرین + مکمل پارکینسونی: 6 رت) تقسیم شدند. سپس گروههای تمرین به مدت 12 هفته برنامه تمرین اینتروال را انجام داده و روزانه 100 میلی گرم بر کیلوگرم وزن بدن کوئرستین مصرف کردند. در نهایت و بعد از آخرین جلسه تمرینی بافت هیپوکامپ برداشته شده و متغیرهای تحقیق با استفاده از روش برادفورد اندازهگیری شدند.
نتایج: نتایج اثرات معنیدار تمرین و عدم تاثیر معنیدار تمرین و مکمل را بر Bax نشان داده است. همچنین نتایج اثرات معنیدار تمرین و تمرین و مکمل و عدم تاثیر معنیدار مکمل را بر bcl-2 معنیدار است. اما اثر اصلی مکمل کروئستین بر bcl-2 نشان داده است.
نتیجهگیری: با توجه به نتایج استفاده از تمرین و تمرین و مکمل کروئستین برای افراد دیابتی زیر نظر متخصص توصیه میشود.
Background & purpose: Parkinson's disease is a progressive neurological disorder caused by the death of neurons in the substantia nigra, the degeneration of dopaminergic neurotransmission, and the presence of alpha-synuclein and protein components in the cell body of neurons. The purpose of this research was to explain the effect of three months of interval aerobic exercise and quercetin supplementation on the concentration Bax and Bcl-2 in the hippocampus of male Parkinsonian model rats.
Materials & Method: To conduct the present experimental research, 28 male Wistar rats were prepared and divided into 5 healthy control groups, 5 Parkinson's control groups, 6 Parkinson's training groups, 6 Parkinson's melatonin groups, and 6 Parkinson's training + Parkinson's supplement groups. were divided Then, the training groups performed the interval training program for 12 weeks and consumed 100 mg/kg of quercetin daily. Finally, after the last training session, the hippocampus tissue was removed and the research variables were measured using the Bradford method.
Results: The results showed the significant effects of training and the lack of significant effects of training and supplementation on Bax. Also, the results of the significant effects of exercise and exercise and supplementation and the lack of significant effect of supplementation on bcl-2 are significant. But the main effect of quercetin supplementation has been shown on bcl-2.
Conclusion: According to the results, the use of exercise and exercise and quercetin supplement is recommended for diabetics under the supervision of a specialist
فهرست منابع
1.Kakran M, Li L, Müller RH. Overcoming the challenge of poor drug solubility. Pharm. Eng. 2012 Jul;32(4):1-7.
2. Shrivastava P, Vaibhav K, Tabassum R, Khan A, Ishrat T, Khan MM, Ahmad A, Islam F, Safhi MM, Islam F. Anti-apoptotic and anti-inflammatory effect of Piperine on 6-OHDA induced Parkinson's rat model. The Journal of Nutritional Biochemistry. 2013 Apr 1;24(4):680-7.
3. Ahmadi M, Abbassi Daloii A, Salehi Kiasari S. Response of Liver Tissue Bax and Bcl-2 Gene Expression to Aerobic Training with L-Carnitine Supplementation in Rats Toxicated by Boldenone. Complementary Medicine Journal. 2020 Mar 10;9(4):3890-901.
4. Antonelli F, Strafella AP. Behavioral disorders in Parkinson's disease: the role of dopamine. Parkinsonism & related disorders. 2014 Jan 1;20:S10-2.
5. Du L, Li J, Chen C, Liu Y. Nanocarrier: A potential tool for future antioxidant therapy. Free radical research. 2014 Sep 1;48(9):1061-9.
6. Chander K, Vaibhav K, Ahmed ME, Javed H, Tabassum R, Khan A, Kumar M, Katyal A, Islam F, Siddiqui MS. Quercetin mitigates lead acetate-induced behavioral and histological alterations via suppression of oxidative stress, Hsp-70, Bak and upregulation of Bcl-2. Food and chemical toxicology. 2014 Jun 1;68:297-306.
7. Hou Y, Zeng Y, Li S, Qi L, Xu W, Wang H, Zhao X, Sun C. Effect of quercetin against dichlorvos induced nephrotoxicity in rats. Experimental and Toxicologic Pathology. 2014 Jul 1;66(4):211-8.
8. Wang DM, Li SQ, Wu WL, Zhu XY, Wang Y, Yuan HY. Effects of long-term treatment with quercetin on cognition and mitochondrial function in a mouse model of Alzheimer’s disease. Neurochemical research. 2014 Aug;39:1533-43.
9. Sriraksa N, Wattanathorn J, Muchimapura S, Tiamkao S, Brown K, Chaisiwamongkol K. Cognitive‐Enhancing Effect of Quercetin in a Rat Model of Parkinson′ s Disease Induced by 6‐Hydroxydopamine. Evidence‐Based Complementary and Alternative Medicine. 2012;2012(1):823206.
10. Tang Y, Li Y, Yu H, Gao C, Liu L, Xing M, Liu L, Yao P. Quercetin attenuates chronic ethanol hepatotoxicity: Implication of “free” iron uptake and release. Food and Chemical Toxicology. 2014 May 1;67:131-8.
11. Merzoug S, Toumi ML, Tahraoui A. Quercetin mitigates Adriamycin-induced anxiety-and depression-like behaviors, immune dysfunction, and brain oxidative stress in rats. Naunyn-Schmiedeberg's archives of pharmacology. 2014 Oct;387:921-33.
12. Mirdar Harijani S, Musavi N, Hamidian G. Effect of endurance swimming training during pregnancy on histology and apoptotic index of rats' liver. Iranian South Medical Journal. 2015 Apr 4;18(1):54-63.
13- Shirvani H, Aslani J, Fallah Mohammadi Z, Arabzadeh E. Short-term effect of low-, moderate-, and high-intensity exercise training on cerebral dopamine neurotrophic factor (CDNF) and oxidative stress biomarkers in brain male Wistar rats. Comparative Clinical Pathology. 2019 Apr 1;28:369-76.
14. Wu SY, Wang TF, Yu L, Jen CJ, Chuang JI, Wu FS, Wu CW, Kuo YM. Running exercise protects the substantia nigra dopaminergic neurons against inflammation-induced degeneration via the activation of BDNF signaling pathway. Brain, behavior, and immunity. 2011 Jan 1;25(1):135-46.
16. Thompson CB. Apoptosis in the pathogenesis and treatment of disease. Science. 1995 Mar 10;267(5203):1456-62.
17.Chen Z, Jiang H, Wan Y, Bi C, Yuan Y. H 2 O 2-induced secretion of tumor necrosis factor-α evokes apoptosis of cardiac myocytes through reactive oxygen species-dependent activation of p38 MAPK. Cytotechnology. 2012 Jan;64:65-73.
18- Haghparast A, Taslimi Z, Ramin M, Azizi P, Khodagholi F, Hassanpour-Ezatti M. Changes in phosphorylation of CREB, ERK, and c-fos induction in rat ventral tegmental area, hippocampus and prefrontal cortex after conditioned place preference induced by chemical stimulation of lateral hypothalamus. Behavioural Brain Research. 2011 Jun 20;220(1):112-8.
19- Habibi P, Alihemmati A, NourAzar A, Yousefi H, Mortazavi S, Ahmadiasl N. Expression of the Mir-133 and Bcl-2 could be affected by swimming training in the heart of ovariectomized rats. Iranian journal of basic medical sciences. 2016 Apr;19(4):381.
20- Ghanbari-Niaki A. Neuregulins response to exercise: a mini review. Annals of Applied Sport Science. 2016 Apr 10;4(1):3-7.
21- Seo H, Park CH, Choi S, Kim W, Jeon BD, Ryu S. Effects of voluntary exercise on apoptosis and cortisol after chronic restraint stress in mice. Journal of exercise nutrition & biochemistry. 2016 Sep;20(3):16.
22- Li F, Shi W, Zhao EY, Geng X, Li X, Peng C, Shen J, Wang S, Ding Y. Enhanced apoptosis from early physical exercise rehabilitation following ischemic stroke. Journal of neuroscience research. 2017 Apr;95(4):1017-24..
23- Tripathi BK, Srivastava AK. Diabetes mellitus: complications and therapeutics. Med Sci Monit. 2006 Jul 1;12(7):130-47.
24- Mooren FC, Bloming D, Lechtermann A, Lerch MM, Volker K. Lymphocyte apoptosis after exhaustive and moderate exercise. Journal of applied physiology. 2002 Jul 1;93(1):147-53.
25- Maulik N, Sasaki H, Addya S, Das DK. Regulation of cardiomyocyte apoptosis by redox-sensitive transcription factors. FEBS letters. 2000 Nov 17;485(1):7-12.
26- Marín-García J, Goldenthal MJ. Mitochondrial centrality in heart failure. Heart failure reviews. 2008 Jun;13:137-50.
27. Sun Y, Cui D, Zhang Z, Zhang T, Shi J, Jin H, Ge Z, Ji L, Ding S. Attenuated oxidative stress following acute exhaustive swimming exercise was accompanied with modified gene expression profiles of apoptosis in the skeletal muscle of mice. Oxidative medicine and cellular longevity. 2016;2016(1):8381242.
28. Aboutaleb N, Shamsaei N, Khaksari M, Erfani S, Rajabi H, Nikbakht F. Pre-ischemic exercise reduces apoptosis in hippocampal CA3 cells after cerebral ischemia by modulation of the Bax/Bcl-2 proteins ratio and prevention of caspase-3 activation. The Journal of Physiological Sciences. 2015 Sep;65:435-43.
29. Santana ET, Serra AJ, Silva JA, Bocalini DS, Barauna VG, Krieger JE, Tucci PJ. Aerobic exercise training induces an anti-apoptotic milieu in myocardial tissue. Motriz: Revista de Educação Física. 2014;20(2):233-8.
30. Jafari A, Pourrazi H, Nikookheslat S, Baradaran B. Effect of exercise training on Bcl-2 and bax gene expression in the rat heart. Gene, Cell and Tissue. 2015 Oct 31;2(4).
31. Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP. Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. American Journal of Physiology-Heart and Circulatory Physiology. 2011 Jan;300(1):H118-24.
32. Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Molecular medicine reports. 2015 Aug 1;12(2):2374-82.
33. Davoodi M, Moosavi H, Nikbakht M. The effect of eight weeks selected aerobic exercise on liver parenchyma and liver enzymes (AST, ALT) of fat liver patients. Univ Med Sci J.2015; 14(1):84-90.
34. Dauer W, Przedborski S. Parkinson's disease: mechanisms and models. Neuron. 2003 Sep 11;39(6):889-909.
35. Lin TK, Liou CW, Chen SD, Chuang YC, Tiao MM, Wang PW, Chen JB, Chuang JH. Mitochondrial dysfunction and biogenesis in the pathogenesis of Parkinson’s disease. Chang Gung Med J. 2009 Nov 1;32(6):589-99.
