اثر محافظتی نانواکسیدمنیزیم در آسیب ایسکمی / رپرفیوژن کلیه بر فاکتور‌های سرمی در موش صحرایی نر
اثر نانواکسیدمنیزیم در آسیب ایسکمی / رپرفیوژن کلیه بر فاکتور‌های سرمی
محورهای موضوعی : بیوشیمی خون و تشخیص نشانگر های زیستی
مرضیه مینایی 1 , اکرم عیدی 2 , پژمان مرتضوی 3 , احمد اصغری 4
1 - گروه زیست شناسی ، واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی ، تهران ، ایران
2 - گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
3 - گروه پاتولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران
4 - گروه علوم بالینی ، دانشکده علوم دامپزشکی تخصصی ، واحد علوم و تحقیقات ، دانشگاه آزاد اسلامی ، تهران ، ایران
کلید واژه: نانواکسیدمنیزیم, ایسکمی رپرفیوژن, کلیه, موش صحرایی,
چکیده مقاله :
زمینه و هدف: در سال‌های اخیر فناوری نانو اشکال جدیدی از مواد را تولید کرده است که مؤثرتر از پیشینیان خود هستند. منیزیم نقش کلیدي در بسیاري از فرآیندهاي بیولوژیکی دارد. این مطالعه با هدف بررسی اثرات نانواکسیدمنیزیم در ایسکمی رپرفیوژن (I/R) کلیوی بعنوان مـشكل شـايع جراحي هـاي عروق كليه يا پيوند كليه، در مدل موش صحرایی نر انجام گرفته است. مواد و روش ها: در این مطالعه القای آسیب I/R از طریق بستن پدیکل کلیوی چپ به مدت 20 دقیقه انجام شد. حیوانات با استفاده از نانو اکسیدمنیزیم (دوزهای 25/1، 5/2 و 5 میلی‌گرم بر کیلوگرم) به مدت 30 روز با استفاده از گاواژ تجویز گردید و سپس نمونه خونی جمع آوري شد و تغییرات آلبومین، توتال پروتئین، اوره و کراتینین سرم بررسی گردید و داده‌هاي حاصل مورد آنالیز آماري قرارگرفت (05/0>p). نتایج: یافته های تحقیق نشان داد که تیمار نانواکسیدمنیزیم به موش‌های صحرایی آسیب دیده‌ی ایسکمی رپرفیوژن کلیه به طور معنی داری سطح اوره و کراتینین سرم را کاهش و میزان آلبومین و توتال پروتیین سرم را افزایش می دهد. نتیجه‌گیری: تیمار نانواکسیدمنیزیم عملکرد کلیه را حفظ کرده و احتمالا توانسته بعنوان یک وازودیلاتور قوی، آسیب هاي وارده به کلیه متعاقب القا ایسکمی رپرفیوژن را کاهش دهد.از آسیب بافتی پس از آسیب I / R در موش جلوگیری می کند.
Background & Aim: In recent years, nanotechnology has produced new forms of materials that are more effective than their predecessors. Magnesium plays a key role in many biological processes. The aim of this study was to investigate the effects of nano magnesium oxide (Nano-MgO) on renal ischemia/reperfusion (I/R) as a common complication of renal vascular surgery or renal transplantation in a male rat model. Materials & Methods: In this study, Induction of I/R injury was done through clamping left renal pedicle for 20 min. Animals were administrated with Nano-MgO (1.25, 2.5 and 5 mg/kg) for 30 days by gavage and then blood samples were collected and changes in serum albumin, total protein, urea and creatinine were examined and the data were statistically analyzed (p <0.05). Results: Our findings showed that Nano-MgO administration to renal I/R injury rats significantly attenuates serum creatinine and urea levels. Also, administration of Nano-MgO increased serum albumin and total protein levels. Conclusion: The Nano-MgO administration preserves renal function and As a potent vasodilator, Nano-MgO may have been able to reduce kidney damage following ischemia-reperfusion induction.
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