بررسی اثرات سولفات مس برروی کیفیت پارامترهای اسپرم ، میزان قطعه قطعه شدن DNA و بافت بیضه موش های بالغ نژاد ویستار
محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی
راحیل جنتی فر
1
,
حمید پیروزمنش
2
,
لیلا ناصرپور
3
1 - گروه پژوهشی بیولوژی تولید مثل، واحد تحقیقات جهاددانشگاهی، قم،ایران
2 - گروه پژوهشی بیولوژی تولید مثل، واحد تحقیقات جهاددانشگاهی، قم،ایران
3 - گروه پژوهشی بیولوژی تولید مثل، واحد تحقیقات جهاددانشگاهی، قم،ایران
کلید واژه: سولفات مس, بافت بیضه, کیفیت اسپرم, قطعه قطعه شدن DNA,
چکیده مقاله :
سولفات مس یکی از مهم ترین آلاینده های زیست محیطی است که دارای توان تولید رادیکال‌های آزاد و استرس اکسیداتیو می‌باشد. هدف از این مطالعه بررسی اثرات سولفات مس برروی کیفیت پارامترهای اسپرم، میزان قطعه قطعه شدن DNA و بافت بیضه موش‌های بالغ نژاد ویستار می‌باشد. روش کار: در این مطالعه تجربی، 30 موش‌های نژاد ویستار بالغ با وزن 250-200 گـرم اسـتفاده شد. موش‌های بالغ تصادفی در 3 گروه کنترل، دریافت کننده سولفات مس با غلظت(mg/kg100) و غلظت(mg/kg200) به مدت زمان 56 روز مورد تیمار قرار گرفتند. در پایان دوره تیمار، وزن بیضه، ثبت و پارامترهای اسپرم بر اساس(WHO2010) بررسی شد.کیفیت کروماتین اسپرم، توسط رنگ آمیزی‌های هسته‌ای آکریدین اورانژ ارزیابی، سطح مالون دی آلدهید اندازه‌گیری و داده‌ها با روش آماریOne-Way ANOVA آنالیز شدند. یافته ها: کیفیت پارامترهای اسپرم در سولفات مس با غلظت mg/kg200 کاهش معنی‌داری داشت(05/0>p ). وزن بیضه در دوز mg/kg200 کاهش معنی‌داری داشت(05/0>p ). قطر لوله‌های منی ساز، سطح هورمون تستوسترون، تعداد سلول های جنسی و میزان تکه‌تکه شدن DNA اسپرم در دوز mg/kg200 کاهش یافت(05/0>p ). غلظت مالون دی آلدهید با دوز mg/kg 200 سولفا ت مس به طور معنی داری افزایش داشت(05/0>p ). نتیجه گیری: این مطالعه نشان می‌دهد که سولفات مس در غلظت بالا باعث ایجاد اثرات مخرب بر روی کیفیت اسپرم و بافت بیضه می‌شود.
Aim: Copper sulfate is one of the most important environmental pollutants that has the ability to produce free radicals and create oxidative stress. The aim of this study was to investigate the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult Wistar rats. Materials and Methods: In this experimental study, 30 adult Wistar rats weighing 250-200 grams were used. Random adult mice were treated in 3 control groups, copper sulfate receptor with concentration (100 mg / kg) and concentration (200 mg / kg) for 56 days. At the end of the treatment period, testicular weight, sperm count and parameters were assessed based on (WHO2010). The quality of sperm chromatin was assessed by acridine uranium nuclear pigments. Malondialdehyde level was measured. Data were analyzed using the One-Way ANOVA statistical method. Results: The quality of sperm parameters in copper sulfate with a concentration of 200 mg / kg decreased significantly (P <0.05). The testicular weight was significantly reduced at a dose of 200 mg / kg (P <0.05). The diameter of the seminiferous tubules, testosterone levels,, germ cell count, and sperm DNA fragmentation rate decreased at a dose of 200 mg / kg (P <0.05). The concentration of malondialdehyde at a dose of 200 mg / kg sulfate copper was significantly increased (P <0.05). Conclusion: This study shows that high concentrations of copper sulfate cause destructive effects on sperm quality, testicular tissue.
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