Optimization of extraction methods for total polyphenolic compounds obtained from rhizomes of Zingiber officinale
محورهای موضوعی : Pharmacognosy
Lida Eberle
1
(Department of Pharmaceutical Chemistry of I.I. Mechnikov, Odessa National University, Odessa, Ukraine)
Alena Kobernik
2
(Department of Pharmaceutical Chemistry of I.I. Mechnikov, Odessa National University, Odessa, Ukraine|Department of Organic and Pharmaceutical Technology, Odessa National Polytechnic University, Odessa, Ukraine)
Aleksandra Aleksandrova
3
(Department of Pharmaceutical Chemistry of I.I. Mechnikov, Odessa National University, Odessa, Ukraine|Department of Organic and Pharmaceutical Technology, Odessa National Polytechnic University, Odessa, Ukraine)
Iryna Kravchenko
4
(Department of Pharmaceutical Chemistry of I.I. Mechnikov, Odessa National University, Odessa, Ukraine|Department of Organic and Pharmaceutical Technology, Odessa National Polytechnic University, Odessa, Ukraine)
کلید واژه: <i>Zingiber officinale</i>, Flavonoids, Extraction methods, Polyphenolic compounds, phytochemical analysis,
چکیده مقاله :
In an alternative medicine, aqueous extracts and decoctions of a ginger root (Ginger –Zingiber officinale Roscoe L., Ginger family – Zingiber aceae) are used as antibacterial, diaphoretic, analgesic, anti-emetic, anti-inflammatory drugs. The aim of our study was to determine the optimal technological conditions of total polyphenolic compounds extraction under different extraction conditions from ginger roots. Ethanol extracts of the plant were obtained by maceration method, Soxleted apparatus and ultrasonic method. Polyphenol compounds and flavonoids were determined with the standard spectrophotometric methods using the Folin-Ciocalteu and differential spectrophotometry (complexation with AlCl3), respectively. It was found that the highest content of polyphenolic compounds and flavonoids from the raw material into the solvent system are determined after sonication followed by subsequent maceration during 10 (total polyphenolic compounds) and 7 days (flavonoids). Their concentration was 2.48 ± 0,029% and 1.3±0,08 %, respectively.
Antolovich, M., Prenzler, P., Robards, K., Ryan, D., 2000. Sample preparation in the determination of phenolic compounds in fruits. Analyst 125(21), 989-1009.
Aizam, K., Ibrahim, B., 2006. Extraction of essential oils from ginger rhizome using steam distillation method Faculty of Chemical & Natural Resources. Engineering University College of Engineering & Technology Malaysia 1(3), 15-25.
Bager, S., Ovesen, L., 2012. Assessment report on Roscoe, rhizoma. London: European Medicines Agency 11(5), 48-49.
Balanchandarn, S., Kentish, S., Mawson, R., 2006. The effect of both the preparation method and season on the super critical extraction of ginger. Sep. Purif. Technol. 48(2), 94-105.
Bartley, J.P., Jacobs A.L., 2000. Effects of drying on flavor compounds in Australian-grown ginger (Zingiber officinale). J. Sci. Food Agr. 80, 209-215.
Chen, H.H., Chung, C.C., Wang, H.Y., Huang, T.C., 2011. Application of Taguchi method to optimize extracted ginger oil in different drying conditions. International Conference on Food Engineering and Biotechnology, IPCBEE IACSIT Press, Singapore 9(3), 310-315.
Chen, H.G., Yu, Y.G., Zeng, O.X., 2002. Study on extraction of flavonoids and alkaloids from lotus leaf. Food Sci. 23(5), 69-71.
Ding, S.H., An, K.J., Zhao, C.P.,Li, Y.G., Guo, Y.H., Wang, Z.F., 2012. Effect of drying methods on volatiles of Chinese ginger (Zingiberofficinale). Food Bioprod. Process. 90(3), 515-524.
Feng, X.P., Cha, Z.Y., Wang, H.S., 2002. Study on the extractive technology of total flavones from foliage of Gingko. Chem. Ind. Corros. Control Sichuan 138(2), 23-26.
Ghasemzadeh, H., Jaafar, A., Rahmat, T., 2010. Identification and concentration of some flavonoid components in Malaysian young ginger (Zingiber officinale Roscoe) varieties by a high performance liquid chromatography method. Molecules 15(9), 31-43.
Hasan, H.A., Raauf, A.M., Razik, B.M., 2012. Chemical composition and antimicrobial activity of the crude extracts isolated from Zingiber Officinale by different solvents. Pharmaceut. Anal. Acta 3(9).
Hu J, Guo Z, Glasius M, Kristensen K, Xiao L, Xu X., 2011. Pressurized liquid extraction of ginger (Zingiber officinale Roscoe) with bioethanol: an efficient and sustainable approach. J. Chromatogr. A, 1218 (34):5765-5773.
Kim, I.G., Kang, S.C., Kim, K.C., Choung, E.S., Zee, O.P., 2008. Screening of estrogenic and antiestrogenic activities from medicinal plants. Environ Toxicol. Pharmacol. 25(3), 75-82.
Kumar, S.M., Sabina, E.P., Nisha, R.T., Ankita, K.I., Anil, D.A., 2013. Evaluation of Zingiber officinale and Curcuma long a rhizome as a crude drug from their ethanolic extract. Department of Applied Chemistry Samrat Ashok Technological Institute. Vidisha (M.P.), India 4(12), 74-76.
Lubberts, E., Berg, W., 2003. Cytokines in the pathogenesis of rheumatoid arthritis and collagen-induced arthritis. Adv. Exp. Med. Biol. 194-202.
Magalhães, L.M., Santos, F., Segundo, M. A., Reis, S. D., Lima, J. L., 2010. Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity. Talanta 8(3), 441-447.
Mandana, B.A., Russly, A.R., Farah, S.T., Liza, M.S., Jinap S.A., Zaidu, I.S., 2011. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Meta spicata L.) leaves. Food Bioprod. Process. 89(1), 67-72.
Mesomo, M.C., Scheer, A.P., Elisa, P.R., Ndiaye, P.M., Corazza, M.L., 2012. Ginger (Zingiber officinale R.) extracts obtained using supercritical CO2 and compressed propene: kinetics and antioxidant activity evaluation. J. Supercrit. Fluids 80, 102-109.
Masullo, M. Montoro, P. Mari, A. Pizza C., Piacente, S. 2015. Medicinal plants in the
treatment of women’s disorders: analytical strategies to assure quality, safety and efficacy. J Pharm. Biomed. Anal. 113, 189-211.
Mishra, R.K., Kumar, A.D., 2012. Pharmacological activity of Zingiber officinale. Int. J. Pharm. Chem. Biol. Sci. 1(3), 1073-1078.
Offei-Oknye, R., Patterson, J., Walker, L., 2015. Processing effects on phytochemical content and antioxidative potential of ginger Zingiber officale. Food Nut. Sci. 6, 445-451.
Padalia, R.C., Verma, R.S., Sah, A.N., Sundaresan, V.B., Sakia, D., 2011. Leaf and rhizome oil composition of Zingiber officinale Roscoe and their antibacterial and antioxidant activities. J. Trad. Med. 6(2), 73-82.
Pavlyuk, I., Stadnytska, N., Jasicka-Misiak, I., GórkaB., Wieczorek, P., Novikov, V., 2015. A study of the chemical composition and biological activity of extracts from wild carrot (Daucuscarota L.) seeds waste. RJPBCS 6(2), 603-611.
Pragasam, S.J., Kumar, S.M., Bhoumik, M.B., Sabina, E.P., Rasool, M.T., 2011. 6-Gingerol, an active ingredient of ginger suppresses monosodium ureatecrystal-induced inflammation: An in vivo and in vitro evaluation. Ann. Biol. Res. 2(3), 200-208.
Rahmani, A.H., Shabrmi, F.M., Aly, S.M., 2014. Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. Int. J. Physiol. Pathophysiol. Pharmacol. 6(2), 125-136.
Ravidran, N., Nirmal Babu, K., 2005. Ginger: the genus Zingiber. NY: CRC Press 553.
Sasidharan, I.C., Menon, A.N., 2015. Comparative chemical composition and antimicrobial activity fresh & dry ginger oils (Zingiber officinale). Int. J. Curr. Pharm. Res. 2(4), 40-43.
Singleton, V.L., Orthofer, R., Lamuela-Raventos, R.M., 1999. Analysis of total phenols and other oxidation substances by means of Folin-Ciocalteu reagent. Methods Enzymol. 299, 152-178.
Supardan, M.D., Fuadi, A.K., Alam, P.N., 2011. Solvent extraction of ginger oleoresin using ultrasound. Makarasains 15(2), 163-167.
Wattanathorn, J., Jittiwat, N., Tongun, S., Muchimapura, K., 2001. Zingiber officinale mitigates brain damage and improves memory impairment in focal cerebral ischemic rat. Evid. Based Complement. Alternat. Med. 5(2), 114-125.
Yang, Z., Yang, W., Peng, Q., 2009. Volatile phytochemical composition of rhizome of ginger after extraction by headspace solid-phase microextraction, petrol ether extraction and steam distillation extraction. Bangladesh J. Pharmacol. 4(2), 136-143.
Zahraa, Al., Elah, A., Naqqash, M., 2013. Evaluation of the activity of crude alkaloids extracts of Zingiber officinale Roscoe, Thymus vulgaris L. and Acacia arabica L. as coagulant agent in lab mice. Biomed. Biotechnol. 13(2), 11-16.
H. Zhou, L. Wei, and H. Lei, 1998. Analysis of essential oil from rhizoma Zingiberis by GC-MS, Zhongguo Zhong Yao Za Zhi, 23(4), 234–256.
